D. Fioretto
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View article: Brillouin Microscopy of Breast tumor Spheroids On‐a‐Chip: Mechanical and Transcriptional Responses to Microfluidic‐Induced Rapid Deformations
Brillouin Microscopy of Breast tumor Spheroids On‐a‐Chip: Mechanical and Transcriptional Responses to Microfluidic‐Induced Rapid Deformations Open
Pioneering a novel integrated photonic platform, Brillouin–Raman Microspectroscopy on‐a‐Chip is presented, which enables real‐time, high resolution analysis of mechano‐chemical dynamics in complex 3D biological systems under controlled def…
View article: Beyond Water Content: Unraveling Stiffness in Hydrated Materials by a Correlative Brillouin–Raman Approach
Beyond Water Content: Unraveling Stiffness in Hydrated Materials by a Correlative Brillouin–Raman Approach Open
Brillouin microscopy is revolutionizing bioimaging by enabling noninvasive, label-free mapping of the microscale mechanical properties of biological samples. However, the lack of a robust physical model to disentangle the significant influ…
View article: Brillouin spectroscopy for accurate assessment of morphological and mechanical characteristics in micro-structured samples
Brillouin spectroscopy for accurate assessment of morphological and mechanical characteristics in micro-structured samples Open
Brillouin spectroscopy has recently attracted attention as a powerful tool for the characterization of the mechanical properties of heterogeneous materials, particularly in the biological and biomedical domains. This study investigates the…
View article: Brillouin scattering from biomedical samples: the challenge of heterogeneity
Brillouin scattering from biomedical samples: the challenge of heterogeneity Open
Brillouin light scattering (BLS), a non-destructive and non-contact technique, offers a powerful tool for probing the micromechanical properties of biological tissues. However, the inherent heterogeneity of biological tissues can pose sign…
View article: Multimodal spectroscopic assessment of mechanical and chemical properties of ABS objects in cultural heritage preservation
Multimodal spectroscopic assessment of mechanical and chemical properties of ABS objects in cultural heritage preservation Open
The 1960s saw the emergence of plastic as an indispensable component in various fields, including art and design. Acrylonitrile-butadiene-styrene (ABS) is widely used by artists and designers for a range of applications including sculpture…
View article: Size and environment: The effect of phonon localization on micro-Brillouin imaging
Size and environment: The effect of phonon localization on micro-Brillouin imaging Open
Specifically designed samples have been analyzed to test the ability of Brillouin spectroscopy to provide reliable mechanical characterization of micro and nano-objects. The selected samples are polymeric films, whose transversal sizes fro…
View article: Brillouin–Raman micro-spectroscopy and machine learning techniques to classify osteoarthritic lesions in the human articular cartilage
Brillouin–Raman micro-spectroscopy and machine learning techniques to classify osteoarthritic lesions in the human articular cartilage Open
In this study, Brillouin and Raman micro-Spectroscopy (BRamS) and Machine Learning were used to set-up a new diagnostic tool for Osteoarthritis (OA), potentially extendible to other musculoskeletal diseases. OA is a degenerative pathology,…
View article: Microscale mechanochemical characterization of drying oil films by in situ correlative Brillouin and Raman spectroscopy
Microscale mechanochemical characterization of drying oil films by in situ correlative Brillouin and Raman spectroscopy Open
Correlative Brillouin and Raman microspectroscopy (BRaMS) is applied for the in situ monitoring of the chemical and physical changes of linseed oil during polymerization. The viscoelastic properties of the drying oil throughout the phase t…
View article: Disentanglement of Multiple Scattering Contribution in Brillouin Microscopy
Disentanglement of Multiple Scattering Contribution in Brillouin Microscopy Open
We provide a new analytic expression and an innovative experimental method to isolate the effect of multiple scattering (MS) in Brillouin investigation of highly turbid media. On the one hand, an analytic model is given to describe the spe…
View article: Hydration Dynamics of Model Peptides with Different Hydrophobic Character
Hydration Dynamics of Model Peptides with Different Hydrophobic Character Open
The multi-scale dynamics of aqueous solutions of the hydrophilic peptide N-acetyl-glycine-methylamide (NAGMA) have been investigated through extended frequency-range depolarized light scattering (EDLS), which enables the broad-band detecti…
View article: Brillouin–Raman microspectroscopy for the morpho-mechanical imaging of human lamellar bone
Brillouin–Raman microspectroscopy for the morpho-mechanical imaging of human lamellar bone Open
Bone has a sophisticated architecture characterized by a hierarchical organization, starting at the sub-micrometre level. Thus, the analysis of the mechanical and structural properties of bone at this scale is essential to understand the r…
View article: Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues Open
Human bone is a specialized tissue with unique material properties, providing mechanical support and resistance to the skeleton and simultaneously assuring capability of adaptation and remodelling. Knowing the properties of such a structur…
View article: Brillouin imaging in turbid samples: the removal of multiple scattering contribution
Brillouin imaging in turbid samples: the removal of multiple scattering contribution Open
We provide a new analytic expression and an innovative experimental method to isolate the effect of multiple scattering (MS) in Brillouin investigation of highly turbid media. On the one hand, an analytic model is given to describe the spe…
View article: Brillouin microscopy for the evaluation of hair micromechanics and effect of bleaching
Brillouin microscopy for the evaluation of hair micromechanics and effect of bleaching Open
Brillouin microscopy is a new form of optical elastography and an emerging technique in mechanobiology and biomedical physics. It was applied here to map the viscoelastic properties of human hair and to determine the effect of bleaching on…
View article: Covalent Immobilization of Proteases on Polylactic Acid for Proteins Hydrolysis and Waste Biomass Protein Content Valorization
Covalent Immobilization of Proteases on Polylactic Acid for Proteins Hydrolysis and Waste Biomass Protein Content Valorization Open
The recovery of the protein component and its transformation into protein hydrolysates, generally carried out chemically, gives great added value to waste biomasses. The production of protein hydrolysates through enzymatic catalysis would …
View article: Data.rar
Data.rar Open
This folder contains all the raw datasets obtained by using Brillouin and Raman microscopy applied to bone imaging.
View article: Viscoelastic properties of biopolymer hydrogels determined by Brillouin spectroscopy: A probe of tissue micromechanics
Viscoelastic properties of biopolymer hydrogels determined by Brillouin spectroscopy: A probe of tissue micromechanics Open
Gelatin hydrogels cover a wide range of microenvironments that are relevant for bioapplications of Brillouin elastography.
View article: Mechano-chemistry of human femoral diaphysis revealed by correlative Brillouin–Raman microspectroscopy
Mechano-chemistry of human femoral diaphysis revealed by correlative Brillouin–Raman microspectroscopy Open
Brillouin–Raman microspectroscopy is presented as an innovative label-free all-optical investigation approachable to characterize the chemical composition and the mechanical properties of human tissues at micrometric resolution. Brillouin …
View article: Transition across a sharp interface: Data from Raman and Brillouin imaging spectroscopy
Transition across a sharp interface: Data from Raman and Brillouin imaging spectroscopy Open
Brillouin and Raman imaging are powerful techniques for the investigation of complex materials and they are widely used in material science and biophysics [1], [2], [3], [4], [5], [6], [7]. When dealing with microstructures, the results in…
View article: Correlative Brillouin and Raman spectroscopy data acquired on single cells
Correlative Brillouin and Raman spectroscopy data acquired on single cells Open
The distribution of chemical species and the mechanical modulation inside a single cell or tissue are of fundamental importance to characterize their physiological activity or their pathological conditions [1-4]. Here we analyse these prop…
View article: Visualization1_Dataset Water_Glass.JPG
Visualization1_Dataset Water_Glass.JPG Open
Brillouin and Raman spectra collected when moving laterally across a water-glass interface at 0.3 micrometer steps using a microscope objective with 60X objective.
View article: Visualization2_Dataset PET_Glycerol.JPG
Visualization2_Dataset PET_Glycerol.JPG Open
Brillouin and Raman spectra collected when moving laterally across a PET-Glycerol interface at 0.3 micrometer steps using a microscope objective with 60X objective.
View article: Brillouin-derived viscoelastic parameters of hydrogel tissue models
Brillouin-derived viscoelastic parameters of hydrogel tissue models Open
Many problems in mechanobiology urgently require characterisation of the micromechanical properties of the fibrous proteins of cells and tissues. Brillouin light scattering has been proposed as a new optical elastography technique to meet …
View article: Brillouin Light Scattering Microspectroscopy for Biomedical Research and Applications: introduction to feature issue
Brillouin Light Scattering Microspectroscopy for Biomedical Research and Applications: introduction to feature issue Open
There has been a marked revival of interest in brillouin light scattering spectroscopy/microscopy over the last decade in regards to applications related to all optically studying the mechanical problems associated with systems of biologic…
View article: Brillouin micro-spectroscopy of subchondral, trabecular bone and articular cartilage of the human femoral head
Brillouin micro-spectroscopy of subchondral, trabecular bone and articular cartilage of the human femoral head Open
Brillouin micro-spectroscopy is applied for investigating the mechanical properties of bone and cartilage tissues of a human femoral head. Distinctive mechanical properties of the cartilage surface, subchondral and trabecular bone are repo…
View article: Morpho-mechanics of human collagen superstructures revealed by all-optical correlative micro-spectroscopies
Morpho-mechanics of human collagen superstructures revealed by all-optical correlative micro-spectroscopies Open
In every biological tissue, morphological and topological properties strongly affect its mechanical features and behaviour, so that ultrastructure, composition and mechanical parameters are intimately connected. Overall, it is their correc…
View article: Brillouin-Raman mapping of natural fibers with spectral moment analysis
Brillouin-Raman mapping of natural fibers with spectral moment analysis Open
Brillouin spectroscopy has emerged as a novel analytical tool for biophotonic research and applications. It operates on a microscopic scale and in the GHz spectral range, providing a new spatial and frequency window for the analysis of the…