Dagmara Szkolnicka
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View article: Standard Protocols for Characterising Primary and In Vitro‐Generated Human Hepatocytes
Standard Protocols for Characterising Primary and In Vitro‐Generated Human Hepatocytes Open
Hepatocyte‐like cells (HLCs) derived from pluripotent stem cells (PSCs) or direct reprogramming are an unlimited source of human hepatocytes for biomedical applications. HLCs are used to model human diseases, develop precise drugs and esta…
View article: Hepatitis E virus RNA‐dependent RNA polymerase is involved in RNA replication and infectious particle production
Hepatitis E virus RNA‐dependent RNA polymerase is involved in RNA replication and infectious particle production Open
Background and Aims Hepatitis E virus (HEV) is one of the most common causes of acute hepatitis worldwide. Its positive‐strand RNA genome encodes three open reading frames (ORF). ORF1 is translated into a large protein composed of multiple…
View article: Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells
Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells Open
PLCs and Li-iPSCs obtained from small pieces of human needle liver biopsies constitute a novel unlimited source for the production of HLCs. Despite the preservation of a liver specific gene expression footprint in Li-iPSCs, the generation …
View article: Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein
Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein Open
<p>Hepatitis E virus (HEV) is one of the most common causes of acute hepatitis and jaundice in the world. Current understanding of the molecular virology and pathogenesis of hepatitis E is incomplete, especially due to the limited av…
View article: Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein
Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein Open
Hepatitis E virus (HEV) is one of the most common causes of acute hepatitis and jaundice in the world. Current understanding of the molecular virology and pathogenesis of hepatitis E is incomplete, especially due to the limited availabilit…
View article: Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein
Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein Open
Hepatitis E virus (HEV) infection is an important cause of acute hepatitis and may lead to chronic infection in immunocompromised patients. Knowledge of the viral life cycle is incomplete due to the limited availability of functional tools…
View article: Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion
Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion Open
Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here, we sho…
View article: Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells
Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells Open
Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease in developed countries. An in vitro NAFLD model would permit mechanistic studies and enable high-throughput therapeutic screening. While hepatic cancer-der…
View article: Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells
Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells Open
View article: Supplementary material from "Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells"
Supplementary material from "Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells" Open
Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease in developed countries. An in vitro NAFLD model would permit mechanistic studies and enable high-throughput therapeutic screening. While hepatic can…
View article: "Palmitoylation Mediates Membrane Association of Hepatitis E Virus ORF3 Protein and is Required for Infectious Particle Secretion"
"Palmitoylation Mediates Membrane Association of Hepatitis E Virus ORF3 Protein and is Required for Infectious Particle Secretion" Open
Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here…
View article: "Palmitoylation Mediates Membrane Association of Hepatitis E Virus ORF3 Protein and is Required for Infectious Particle Secretion"
"Palmitoylation Mediates Membrane Association of Hepatitis E Virus ORF3 Protein and is Required for Infectious Particle Secretion" Open
Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here…
View article: MicroRNA regulation of drug metabolism in stem cell - derived hepatocytes
MicroRNA regulation of drug metabolism in stem cell - derived hepatocytes Open
The liver is a multi-functional and highly regenerative organ. While resilient, the liver
\nis susceptible to organ damage and failure. In both the acute and chronic settings liver
\ndisease has dire consequences for health. A common cause…
View article: Reducing Hepatocyte Injury and Necrosis in Response to Paracetamol Using Noncoding RNAs
Reducing Hepatocyte Injury and Necrosis in Response to Paracetamol Using Noncoding RNAs Open
The liver performs multiple functions within the human body. It is composed of numerous cell types, which play important roles in organ physiology. Our study centers on the major metabolic cell type of the liver, the hepatocyte, and its su…
View article: Concise Review: Advances in Generating Hepatocytes from Pluripotent Stem Cells for Translational Medicine
Concise Review: Advances in Generating Hepatocytes from Pluripotent Stem Cells for Translational Medicine Open
The liver is one of the major organs in the human body. Severe or prolonged exposure of the liver to different factors may cause life-threatening disease, which necessitates donor organ transplantation. While orthotopic liver transplantati…
View article: Fluid shear stress modulation of hepatocyte-like cell function
Fluid shear stress modulation of hepatocyte-like cell function Open
Freshly isolated human adult hepatocytes are considered to be the gold standard tool for in vitro studies. However, primary hepatocyte scarcity, cell cycle arrest and the rapid loss of cell phenotype limit their widespread deployment. Huma…
View article: Corrigendum to “Gene networks and transcription factor motifs defining the differentiation of human stem cells into hepatocyte-like cells” [J Hepatol 2015;63:934–942]
Corrigendum to “Gene networks and transcription factor motifs defining the differentiation of human stem cells into hepatocyte-like cells” [J Hepatol 2015;63:934–942] Open
Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cellsJournal of HepatologyVol. 63Issue 4PreviewPrimary human hepatocytes represent a well-established tool in pharmacology and to…
View article: Recombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes
Recombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes Open
View article: Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015)
Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015) Open
One major obstacle to the routine deployment of stem cell-derived cells is their instability in culture. On page 1820 David C. Hay and co-workers describe the use of a synthetic polymer surface. The image shows stem cell-derived hepatocyte…
View article: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance
Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance Open
In theory, pluripotent stem cells can give rise to all somatic cell types found in the human body. The ability to generate renewable sources of human cells has enormous potential to improve human health and wealth. One major obstacle to th…
View article: Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells
Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells Open