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View article: Pleomorphism in Wild‐Type and Engineered PP7 Virus‐Like Particles
Pleomorphism in Wild‐Type and Engineered PP7 Virus‐Like Particles Open
Virus‐like particles (VLPs) find applications across many different fields, aided by their stability, capacity for large‐scale production, and presumed structural homogeneity. These attributes are a result of their highly efficient self‐as…
View article: Understanding the infection mechanism of Φ8 using infection method in <i>Pseudomonas syringae</i> pv<i>. Phaseolicola</i> strain: <i>In-situ</i> CryoET analysis
Understanding the infection mechanism of Φ8 using infection method in <i>Pseudomonas syringae</i> pv<i>. Phaseolicola</i> strain: <i>In-situ</i> CryoET analysis Open
The Cystoviridae family strain Φ8 is an ideal model for studying the virus-host interactions and viral assembly mechanisms. In this study, we explored Φ8 infection kinetics in P. syringae pv. phaseolicola host cells using a combination of …
View article: Overcoming air-water interface-induced artifacts in Cryo-EM with protein nanocrates
Overcoming air-water interface-induced artifacts in Cryo-EM with protein nanocrates Open
Contact with the air-water interface can bias the orientation of macromolecules during cryo-EM sample preparation, leading to uneven sample distribution, preferred orientation, and damage to the molecules of interest. To prevent this, we d…
View article: A multiscale imaging approach to studying the harpoon-like invasion organelle from microsporidian pathogens
A multiscale imaging approach to studying the harpoon-like invasion organelle from microsporidian pathogens Open
Microsporidia are tiny, single-celled parasites similar to fungi that infect a wide range of animal species, from worms and honey bees to humans. In humans, these opportunistic pathogens can cause life-threatening infections in immunocompr…
View article: Overcoming air-water interface-induced artifacts in Cryo-EM with protein nanocrates
Overcoming air-water interface-induced artifacts in Cryo-EM with protein nanocrates Open
Contact with the air-water interface can bias the orientation of macromolecules during cryo-EM sample preparation, leading to uneven sample distribution, preferred orientation, and damage to the molecules of interest. To prevent this, we d…
View article: Mystique, a broad host range Acinetobacter phage, reveals the impact of culturing conditions on phage isolation and infectivity
Mystique, a broad host range Acinetobacter phage, reveals the impact of culturing conditions on phage isolation and infectivity Open
With the global rise of antimicrobial resistance, phage therapy is increasingly re-gaining traction as a strategy to treat bacterial infections. For phage therapy to be successful however, we first need to isolate appropriate candidate pha…
View article: Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites
Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites Open
Microsporidia are divergent fungal pathogens that employ a unique harpoon-like apparatus called the polar tube (PT) to invade host cells. The long PT is fired out of the microsporidian spore over the course of just a few hundred millisecon…
View article: Identification and cryoEM Structure Determination of Escherichia Phage YDC107 Tail Found in a Bacteria-Contaminated Buffer
Identification and cryoEM Structure Determination of Escherichia Phage YDC107 Tail Found in a Bacteria-Contaminated Buffer Open
Cryo-electron microscopy data analysis can yield multiple structures from a single heterogeneous dataset. Here, we show a workflow we used for the identification of a contaminant from a cryoEM grid without prior knowledge of protein sequen…
View article: Multi-species cryoEM calibration and workflow verification standard
Multi-species cryoEM calibration and workflow verification standard Open
Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with c…
View article: Multi-species cryoEM calibration and workflow verification standard
Multi-species cryoEM calibration and workflow verification standard Open
Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with c…
View article: Cryo-ET reveals the<i>in situ</i>architecture of the polar tube invasion apparatus from microsporidian parasites
Cryo-ET reveals the<i>in situ</i>architecture of the polar tube invasion apparatus from microsporidian parasites Open
Microsporidia are divergent fungal pathogens that employ a harpoon-like apparatus called the polar tube (PT) to invade host cells. The PT architecture and its association with neighboring organelles remain poorly understood. Here, we use c…
View article: Environmental differences impact<i>Acinetobacter baumannii</i>phage isolation and infectivity
Environmental differences impact<i>Acinetobacter baumannii</i>phage isolation and infectivity Open
With the global rise of antimicrobial resistance, phage therapy is increasingly re-gaining traction as a strategy to treat bacterial infections. For phage therapy to be successful however, we first need to isolate appropriate candidate pha…
View article: Recent advances in infectious disease research using cryo-electron tomography
Recent advances in infectious disease research using cryo-electron tomography Open
With the increasing spread of infectious diseases worldwide, there is an urgent need for novel strategies to combat them. Cryogenic sample electron microscopy (cryo-EM) techniques, particularly electron tomography (cryo-ET), have revolutio…
View article: Exploring the Landscape of the PP7 Virus-like Particle for Peptide Display
Exploring the Landscape of the PP7 Virus-like Particle for Peptide Display Open
Self-assembling virus-like particles (VLPs) can tolerate a wide degree of genetic and chemical manipulation to their capsid protein to display a foreign molecule polyvalently. We previously reported the successful incorporation of foreign …
View article: Modern Tools for <i>In-situ</i> Tomography
Modern Tools for <i>In-situ</i> Tomography Open
CryoEM in-situ investigations face challenges in every step of the process from grid preparation and lamellae milling to data acquisition and processing.Here, we describe a set of tools and approaches that we use at the National Center for…
View article: In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method
In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method Open
Cryo-focused ion beam (FIB) milling of vitrified specimens is emerging as a powerful method for in situ specimen preparation. It allows for the preservation of native and near-native conditions in cells, and can reveal the molecular struct…
View article: National Center for In-situ Tomographic Ultramicroscopy at New York Structural Biology Center
National Center for In-situ Tomographic Ultramicroscopy at New York Structural Biology Center Open
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View article: Waffle Method: A general and flexible approach for improving throughput in FIB-milling
Waffle Method: A general and flexible approach for improving throughput in FIB-milling Open
Cryo-FIB/SEM combined with cryo-ET has emerged from within the field of cryo-EM as the method for obtaining the highest resolution structural information of complex biological samples in-situ in native and non-native environments. However,…
View article: Time-resolved CryoEM Using Spotiton
Time-resolved CryoEM Using Spotiton Open
An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the 'Save PDF' action button.
View article: Time-resolved cryoEM using Spotiton
Time-resolved cryoEM using Spotiton Open
We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surfa…
View article: Benchmarking cryo-EM Single Particle Analysis Workflow
Benchmarking cryo-EM Single Particle Analysis Workflow Open
Cryo electron microscopy facilities running multiple instruments and serving users with varying skill levels need a robust and reliable method for benchmarking both the hardware and software components of their single particle analysis wor…
View article: Benchmarking cryo-EM single particle analysis workflow
Benchmarking cryo-EM single particle analysis workflow Open
Cryo electron microscopy facilities running multiple instruments and serving users with varying skill levels need a robust and reliable method for benchmarking both the hardware and software components of their single particle analysis wor…