Daisuke Doi
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View article: Transplantation of Human iPS Cell-derived Cerebral Cortical Neurons Promotes Fine Motor Recovery in a Female Mouse Model of Ischemic Stroke
Transplantation of Human iPS Cell-derived Cerebral Cortical Neurons Promotes Fine Motor Recovery in a Female Mouse Model of Ischemic Stroke Open
Background Stroke is a leading global health concern, with cerebral infarction accounting for 62% of cases. Despite advances in acute-phase treatments, functional impairments such as motor deficits remain prevalent. This study investigates…
View article: Control of immune response in an iPSC-based allogeneic cell therapy clinical trial for Parkinson’s disease
Control of immune response in an iPSC-based allogeneic cell therapy clinical trial for Parkinson’s disease Open
Because the central nervous system (CNS) is an immune-privileged organ, it requires different immunosuppression strategies for cell therapies using induced pluripotent stem cells (iPSCs) compared with ones for organ transplantations. We re…
View article: Effect of Continuous Intake of Onion Powder on Oxidative Stress Biomarkers and Skeletal Muscle Maintenance in Elderly Bedridden Individuals:A Pilot Study
Effect of Continuous Intake of Onion Powder on Oxidative Stress Biomarkers and Skeletal Muscle Maintenance in Elderly Bedridden Individuals:A Pilot Study Open
The number of bedridden elderly is substantially increasing in our aging society. Bedridden patients experience muscle atrophy due to prolonged inactivity. We are concerned about whether the antioxidative flavonoid quercetin can prevent di…
View article: Progranulin enhances the engraftment of transplanted human iPS cell-derived cerebral neurons
Progranulin enhances the engraftment of transplanted human iPS cell-derived cerebral neurons Open
Cerebral organoids (COs) in cell replacement therapy offer a viable approach to reconstructing neural circuits for individuals suffering from stroke or traumatic brain injuries. Successful transplantation relies on effective engraftment an…
View article: Cryopreservation of Induced Pluripotent Stem Cell-Derived Dopaminergic Neurospheres for Clinical Application
Cryopreservation of Induced Pluripotent Stem Cell-Derived Dopaminergic Neurospheres for Clinical Application Open
Background: Pluripotent stem cell (PSC)-derived dopaminergic (DA) neurons are an expected source of cell therapy for Parkinson’s disease. The transplantation of cell aggregates or neurospheres, instead of a single cell suspension has sever…
View article: Cell transplantation therapy using induced pluripotent stem cell-derived dopaminergic progenitors for Parkinson's disease
Cell transplantation therapy using induced pluripotent stem cell-derived dopaminergic progenitors for Parkinson's disease Open
パーキンソン病に対する細胞移植治療は、胎児細胞移植の臨床試験で症例によっては有効であるという結果をもとに、ES細胞やiPS細胞などの多能性幹細胞を細胞供給源として研究が進められてきた。最近、世界で多能性幹細胞を使用した細胞移植治療の臨床試験が開始されており、筆者らは2018年からiPS細胞を使用した医師主導治験を開始した。本稿ではパーキンソン病に対する細胞移植治療の歴史、多能性幹細胞を用いた細胞移植治療の開発経緯と、臨床試験での適応、移植方法、リスクについて概説する。
View article: Clinically compatible differentiation protocol for human pluripotent stem cell-derived dopaminergic progenitor cells
Clinically compatible differentiation protocol for human pluripotent stem cell-derived dopaminergic progenitor cells Open
Cell replacement therapy with human pluripotent stem cells has the potential to be a new therapy for Parkinson’s disease (PD). This protocol induces human induced pluripotent stem cells (iPSCs) to dopaminergic progenitor cells (DAPs) as cl…
View article: MHC matching improves engraftment of iPSC-derived neurons in non-human primates
MHC matching improves engraftment of iPSC-derived neurons in non-human primates Open
The banking of human leukocyte antigen (HLA)-homozygous-induced pluripotent stem cells (iPSCs) is considered a future clinical strategy for HLA-matched cell transplantation to reduce immunological graft rejection. Here we show the efficacy…
View article: Serum CA 125 Level after Neoadjuvant Chemotherapy is Predictive of Prognosis and Debulking Surgery Outcomes in Advanced Epithelial Ovarian Cancer
Serum CA 125 Level after Neoadjuvant Chemotherapy is Predictive of Prognosis and Debulking Surgery Outcomes in Advanced Epithelial Ovarian Cancer Open
Recently, neoadjuvant chemotherapy followed by interval debulking surgery (NACT-IDS) has been recommended for selected patients with International Federation of Gynecology and Obstetrics (FIGO) stage III or IV disease and bulky tumors. The…
View article: Estradiol Facilitates Functional Integration of iPSC-Derived Dopaminergic Neurons into Striatal Neuronal Circuits via Activation of Integrin α5β1
Estradiol Facilitates Functional Integration of iPSC-Derived Dopaminergic Neurons into Striatal Neuronal Circuits via Activation of Integrin α5β1 Open
For cell transplantation therapy for Parkinson's disease (PD) to be realized, the grafted neurons should be integrated into the host neuronal circuit to restore the lost neuronal function. Here, using wheat-germ agglutinin-based transsynap…
View article: iPS Cell-derived Cell Therapy for Parkinson’s Disease
iPS Cell-derived Cell Therapy for Parkinson’s Disease Open
パーキンソン病に対して，ヒトiPS細胞由来ドパミン神経前駆細胞を用いた細胞移植治療の臨床試験を計画している．iPS細胞由来の移植片の安全性と有効性を検討する目的で，少数例の患者を対象に，頭部MRIおよびPET検査，スコアによる神経症状の評価を行う予定である．臨床試験では，専用の細胞調製施設で作製された医療用iPS細胞ストックを用いる．種々の低分子化合物やサイトカインを加えた培地でiPS細胞を分化誘導し，セルソーティングの手法によりドパミン神経前駆細胞を濃縮し，さらに浮遊培養…
View article: Effects of dietary soy protein on skeletal muscle volume and strength in humans with various physical activities
Effects of dietary soy protein on skeletal muscle volume and strength in humans with various physical activities Open
The supplementation of soy protein would be one of the effective foods which prevent the skeletal muscle atrophy caused by immobilization or unloading.
View article: Challenges towards Stem Cell Therapy for Parkinson's Disease
Challenges towards Stem Cell Therapy for Parkinson's Disease Open
パーキンソン病に対して胎児中脳腹側細胞移植の効果が報告されているが, 移植細胞の量的・質的問題があり標準治療にはなっていない. ES/iPS細胞を用いた細胞移植ではこれらの課題を解決できる可能性がある. さらに, iPS細胞を用いた場合には自家移植が可能である. われわれはヒトES細胞由来のドパミン神経がサルのパーキンソン病モデルに生着・機能することを示した. また分化誘導法の改良とソーティングにより効率よく大量のドパミン神経前駆細胞を作製し, ヒトiPS細胞由来のドパミン…