Dan Cappabianca
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View article: Low-dose radiation by radiopharmaceutical therapy enhances GD2 <i>TRAC</i> -CAR T cell efficacy in localized neuroblastoma
Low-dose radiation by radiopharmaceutical therapy enhances GD2 <i>TRAC</i> -CAR T cell efficacy in localized neuroblastoma Open
Chimeric antigen receptor (CAR) T cells have limited efficacy against solid tumors including neuroblastoma. Here, we evaluated whether low-dose radiation delivered by radiopharmaceutical therapy (RPT), known to potentiate immune checkpoint…
View article: Longitudinal and continuous label-free monitoring of glioblastoma patient-derived tumor spheroid treatment response using quantitative oblique back illumination microscopy
Longitudinal and continuous label-free monitoring of glioblastoma patient-derived tumor spheroid treatment response using quantitative oblique back illumination microscopy Open
Glioblastoma (GBM) is an aggressive brain tumor with limited treatment options and poor patient survival, underscoring the need for novel therapeutic strategies and improved preclinical models. Patient-derived tumor spheroids (PDTSs) offer…
View article: Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPT
Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPT Open
View article: Low Dose Radiation by Radiopharmaceutical Therapy Enhances GD2<i>TRAC-</i>CAR T Cells Efficacy in Localized Neuroblastoma
Low Dose Radiation by Radiopharmaceutical Therapy Enhances GD2<i>TRAC-</i>CAR T Cells Efficacy in Localized Neuroblastoma Open
Background While chimeric antigen receptor (CAR) T cells have achieved significant success against hematological malignancies, efficacy against neuroblastoma has been limited. Virus-free CRISPR-edited GD2 TRAC- CAR T cells have been develo…
View article: Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells
Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells Open
Efficient engineering of T cells to express exogenous tumor-targeting receptors such as chimeric antigen receptors (CARs) or T-cell receptors (TCRs) is a key requirement of effective adoptive cell therapy for cancer. Genome editing technol…
View article: Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence
Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence Open
Manufacturing chimeric antigen receptor (CAR) T cell therapies is complex, with limited understanding of how medium composition impacts T cell phenotypes. CRISPR-Cas9 ribonucleoproteins can precisely insert a CAR sequence while disrupting …
View article: Metabolic priming of GD2<i>TRAC</i>-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence
Metabolic priming of GD2<i>TRAC</i>-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence Open
Manufacturing Chimeric Antigen Receptor (CAR) T cell therapies is complex, with limited understanding of how media composition impact T-cell phenotypes. CRISPR/Cas9 ribonucleoproteins can precisely insert a CAR sequence while disrupting th…
View article: <i>In vitro</i> encapsulation and expansion of T and CAR-T cells using 3D synthetic thermo-responsive matrices
<i>In vitro</i> encapsulation and expansion of T and CAR-T cells using 3D synthetic thermo-responsive matrices Open
This work employed a biocompatible and synthetic-based thermo-responsive material with tailored mechanical properties as a potential macro-scale scaffold to support ex vivo T and CAR-T cell encapsulation and culture.
View article: Comparative Study of the Effect of Radiation Delivered by Lutetium-177 or Actinium-225 on Anti-GD2 Chimeric Antigen Receptor T Cell Viability and Functions
Comparative Study of the Effect of Radiation Delivered by Lutetium-177 or Actinium-225 on Anti-GD2 Chimeric Antigen Receptor T Cell Viability and Functions Open
Background and purpose. Chimeric antigen receptor (CAR) T cells have been relatively ineffective against solid tumors. Low-dose radiation which can be delivered to multiple sites of metastases by targeted radionuclide therapy (TRT) can eli…
View article: 391 Development of a GMP-compatible, virus-free CRISPR CAR T cell manufacturing process
391 Development of a GMP-compatible, virus-free CRISPR CAR T cell manufacturing process Open
Background Chimeric antigen receptor (CAR) T-cells are engineered immune cells that can be taken from a patient and redirected to fight cancer. Recently, we developed a virus-free process to create anti-GD2 CAR T cells using CRISPR-Cas9 ri…
View article: Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression
Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression Open
Background Chimeric antigen receptor (CAR) T cells have demonstrated high clinical response rates against hematological malignancies (e.g., CD19+ cancers) but have shown limited activity in patients with solid tumors. Recent work showed th…