Daniel B. Mihaylov
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View article: GABA<sub>A</sub> receptor gating imaged on the millisecond timescale
GABA<sub>A</sub> receptor gating imaged on the millisecond timescale Open
Type-A γ-aminobutyric receptors (GABA A Rs) are fast pentameric ligand gated ion channels (pLGICs) 1–5 . Within a millisecond, saturating agonist concentrations trigger activity bursts consisting of high-frequency fluctuations between cond…
View article: Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV
Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV Open
Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report sub-3 Å resolution structures using the 100 keV Tundra cryo-TEM, equipped with the Falcon …
View article: Sub-3 Å Resolution Protein Structure Determination by Single-Particle Cryo-EM at 100 keV
Sub-3 Å Resolution Protein Structure Determination by Single-Particle Cryo-EM at 100 keV Open
View article: One-step nanoscale expansion microscopy reveals individual protein shapes
One-step nanoscale expansion microscopy reveals individual protein shapes Open
View article: Structure determination by cryoEM at 100 keV
Structure determination by cryoEM at 100 keV Open
Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV—i…
View article: Visualizing proteins by expansion microscopy
Visualizing proteins by expansion microscopy Open
Fluorescence imaging is one of the most versatile and widely-used tools in biology 1 . Although techniques to overcome the diffraction barrier were introduced more than two decades ago, and the nominal attainable resolution kept improving …
View article: Architecture of cell–cell junctions in situ reveals a mechanism for bacterial biofilm inhibition
Architecture of cell–cell junctions in situ reveals a mechanism for bacterial biofilm inhibition Open
Significance Pseudomonas aeruginosa bacteria form antibiotic-tolerant biofilms that pose significant challenges in clinical settings. Overcoming these challenges requires fundamental insights into how biofilms are formed, combined with inn…
View article: Architecture of cell-cell junctions in situ reveals a mechanism for bacterial biofilm inhibition
Architecture of cell-cell junctions in situ reveals a mechanism for bacterial biofilm inhibition Open
Many bacteria, including the major human pathogen Pseudomonas aeruginosa , are naturally found in multicellular, antibiotic-tolerant biofilm communities, where cells are embedded in an extracellular matrix of polymeric molecules. Cell-cell…