Daniel Eichinger
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View article: Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles
Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles Open
We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate. First in shake flasks, then in bioreactors,…
View article: A reverse phase HPLC method for the quantification of HIV gp145 glycoprotein levels in cell culture supernatants
A reverse phase HPLC method for the quantification of HIV gp145 glycoprotein levels in cell culture supernatants Open
A reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the quantitative determination of recombinant HIV-1 gp145 produced in CHO-K1 cells, as measured directly in culture supernatants. Samples were dilute…
View article: Development and application of a high-content virion display human GPCR array
Development and application of a high-content virion display human GPCR array Open
Human G protein-coupled receptors (GPCRs) respond to various ligands and stimuli. However, GPCRs rely on membrane for proper folding, making their biochemical properties difficult to study. By displaying GPCRs in viral envelopes, we fabric…
View article: A toolbox of immunoprecipitation-grade monoclonal antibodies against human transcription factors
A toolbox of immunoprecipitation-grade monoclonal antibodies against human transcription factors Open
A key component to overcoming the reproducibility crisis in biomedical research is the development of readily available, rigorously validated and renewable protein affinity reagents. As part of the NIH Protein Capture Reagents Program (PCR…
View article: Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP
Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP Open
Altogether, our characterizations of this new methodology show that FLIP is an appealing and reliable tool for any application of high-throughput IP.
View article: Regenerative biosensor chips based on switchable mutants of avidin—A systematic study
Regenerative biosensor chips based on switchable mutants of avidin—A systematic study Open
Biotinylated bait molecules can be immobilized on biotinylated sensor chips by formation of biotin–avidin–biotin bridges which are very stable when using wild-type (strept)avidin. Stable immobilization of biotinylated baits is important fo…
View article: Additional file 1: Figure S1. of Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP
Additional file 1: Figure S1. of Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP Open
Mass spectrometry of tryptic peptides of YFP fusion proteins recovered from upper and lower bands of HES-1 expressed the HuEV-A vector. Figure S2. Mass spectrometry of tryptic peptides of YFP fusion proteins recovered from upper and lower …