Daniel J. Owens
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View article: ¹³C labelled glucose-fructose show greater exogenous and whole-body CHO oxidation and lower O₂ cost of running at 120 vs 60 & 90 g·h⁻¹ in elite male marathoners
¹³C labelled glucose-fructose show greater exogenous and whole-body CHO oxidation and lower O₂ cost of running at 120 vs 60 & 90 g·h⁻¹ in elite male marathoners Open
We evaluated the effects of carbohydrate (CHO) ingestion at rates of 60 (maltodextrin:fructose ratio 1:0), 90 (2:1), and 120 (1:1) g·h⁻¹ on whole body substrate metabolism, exogenous CHO oxidation (via U- 13 C enriched glucose-fructose dri…
View article: Dynamic proteome profiling uncovers age-related impairments in proteostasis and the protective effects of resistance exercise in human skeletal muscle
Dynamic proteome profiling uncovers age-related impairments in proteostasis and the protective effects of resistance exercise in human skeletal muscle Open
A loss of proteostasis is a primary hallmark of ageing that has emerged from mechanistic studies in model organisms, but little is currently known about changes to proteostasis in the muscle of older humans. We used stable isotope labellin…
View article: Repeated disuse atrophy imprints a molecular memory in skeletal muscle: transcriptional resilience in young adults and susceptibility in aged muscle
Repeated disuse atrophy imprints a molecular memory in skeletal muscle: transcriptional resilience in young adults and susceptibility in aged muscle Open
Disuse-induced muscle atrophy is common after illness, injury, or falls and becomes increasingly frequent with ageing. Whether skeletal muscle retains a “memory” of repeated disuse remains unknown. We investigated repeated lower-limb immob…
View article: Acute nutritional ketosis during early recovery from aerobic exercise does not affect skeletal muscle transcriptomic response in humans
Acute nutritional ketosis during early recovery from aerobic exercise does not affect skeletal muscle transcriptomic response in humans Open
Purpose Nutritional ketosis is purported to enhance skeletal muscle recovery and adaptation to exercise, yet precise adaptive mechanisms are unknown. We investigated the post-exercise molecular response to ketone monoesters (KME) in skelet…
View article: Current Practice in the Measurement and Management of Vitamin D Status in Elite Sport and Parasport
Current Practice in the Measurement and Management of Vitamin D Status in Elite Sport and Parasport Open
The field of vitamin D research in sport has stagnated, with a notable lack of new evidence challenging existing paradigms. Despite well‐documented consequences of vitamin D deficiency in athletes, gaps remain in understanding the feasibil…
View article: Post‐Match Recovery Responses in Italian Serie A Youth Soccer Players: Effects of Manipulating Training Load 48 h After Match Play
Post‐Match Recovery Responses in Italian Serie A Youth Soccer Players: Effects of Manipulating Training Load 48 h After Match Play Open
This study described the recovery responses following match play and examined the effects of manipulating training load 48 h post‐match in Italian Serie A youth soccer players. Forty‐eight players were assessed using the countermovement ju…
View article: Muscle soreness but not neuromuscular fatigue responses following downhill running differ according to the number of exercise bouts
Muscle soreness but not neuromuscular fatigue responses following downhill running differ according to the number of exercise bouts Open
Repeated sessions of eccentric‐biased exercise promote strength gains through neuromuscular adaptation. However, it remains unclear whether increasing the number of these sessions can mitigate the extent of neuromuscular fatigue and exerci…
View article: Comparative transcriptomics of broad‐spectrum and synthetic cannabidiol treated <scp>C</scp>2<scp>C</scp>12 skeletal myotubes
Comparative transcriptomics of broad‐spectrum and synthetic cannabidiol treated <span>C</span>2<span>C</span>12 skeletal myotubes Open
Cannabidiol (CBD) is widely used in sports for recovery, pain management, and sleep improvement, yet its effects on muscle are not well understood. This study aimed to determine the transcriptional response of murine skeletal muscle myotub…
View article: Does a broad‐spectrum cannabidiol supplement improve performance in a 10‐min cycle ergometer performance‐test?
Does a broad‐spectrum cannabidiol supplement improve performance in a 10‐min cycle ergometer performance‐test? Open
Cannabidiol (CBD) is a non‐intoxicating phytocannabinoid which has been proposed to possess anti‐inflammatory and analgesic properties. Given the potential for perceptions of pain to limit exercise performance, the aim of the present study…
View article: An exercise physiologist's guide to metabolomics
An exercise physiologist's guide to metabolomics Open
The field of exercise physiology has undergone significant technological advancements since the pioneering works of exercise physiologists in the early to mid‐20th century. Historically, the ability to detect metabolites in biofluids from …
View article: Fluorescent characterization of differentiated myotubes using flow cytometry
Fluorescent characterization of differentiated myotubes using flow cytometry Open
Flow cytometry is routinely used in the assessment of skeletal muscle progenitor cell (myoblast) populations. However, a full gating strategy, inclusive of difficult to interpret forward and side scatter data, which documents cytometric an…
View article: Reliability and sensitivity to change of post-match physical performance measures in elite youth soccer players
Reliability and sensitivity to change of post-match physical performance measures in elite youth soccer players Open
Introduction To effectively monitor post-match changes in physical performance, valid, reliable and practical measures which are sensitive to change are required. This study aimed to quantify test-retest reliability and sensitivity to chan…
View article: Environmental heat stress offsets adaptation associated with carbohydrate periodization in trained male triathletes
Environmental heat stress offsets adaptation associated with carbohydrate periodization in trained male triathletes Open
Purpose Carbohydrate (CHO) intake periodization via the sleep low train low (SL‐TL) diet–exercise model increases fat oxidation during exercise and may enhance endurance‐training adaptation and performance. Conversely, training under envir…
View article: Acute heat stress amplifies exercise‐induced metabolomic perturbations and reveals variation in circulating amino acids in endurance‐trained males
Acute heat stress amplifies exercise‐induced metabolomic perturbations and reveals variation in circulating amino acids in endurance‐trained males Open
Using untargeted metabolomics, we aimed to characterise the systemic impact of environmental heat stress during exercise. Twenty‐three trained male triathletes ( = 64.8 ± 9.2 ml kg min −1 ) completed a 30‐min exercise test in hot (35°C) an…
View article: Commentaries on Viewpoint: Hoping for the best, prepared for the worst: can we perform remote data collection in sport sciences?
Commentaries on Viewpoint: Hoping for the best, prepared for the worst: can we perform remote data collection in sport sciences? Open
View article: PCM1 labeling reveals myonuclear and nuclear dynamics in skeletal muscle across species
PCM1 labeling reveals myonuclear and nuclear dynamics in skeletal muscle across species Open
Myonuclei transcriptionally regulate muscle fibers during homeostasis and adaptation to exercise. Their subcellular location and quantity are important when characterizing phenotypes of myopathies, the effect of treatments, and understandi…
View article: The time course of different neuromuscular adaptations to short-term downhill running training and their specific relationships with strength gains
The time course of different neuromuscular adaptations to short-term downhill running training and their specific relationships with strength gains Open
View article: Three weeks of a home-based “sleep low-train low” intervention improves functional threshold power in trained cyclists: A feasibility study
Three weeks of a home-based “sleep low-train low” intervention improves functional threshold power in trained cyclists: A feasibility study Open
Background “Sleep Low-Train Low” is a training-nutrition strategy intended to purposefully reduce muscle glycogen availability around specific exercise sessions, potentially amplifying the training stimulus via augmented cell signalling. T…
View article: “Fuel for the Damage Induced”: Untargeted Metabolomics in Elite Rugby Union Match Play
“Fuel for the Damage Induced”: Untargeted Metabolomics in Elite Rugby Union Match Play Open
The metabolic perturbations caused by competitive rugby are not well characterized. Our aim is to utilize untargeted metabolomics to develop appropriate interventions, based on the metabolic fluctuations that occur in response to this coll…
View article: Four Weeks of Probiotic Supplementation Alters the Metabolic Perturbations Induced by Marathon Running: Insight from Metabolomics
Four Weeks of Probiotic Supplementation Alters the Metabolic Perturbations Induced by Marathon Running: Insight from Metabolomics Open
Few data are available that describe how probiotics influence systemic metabolism during endurance exercise. Metabolomic profiling of endurance athletes will elucidate mechanisms by which probiotics may confer benefits to the athlete. In t…
View article: Carbohydrate improves exercise capacity but does not affect subcellular lipid droplet morphology, AMPK and p53 signalling in human skeletal muscle
Carbohydrate improves exercise capacity but does not affect subcellular lipid droplet morphology, AMPK and p53 signalling in human skeletal muscle Open
Key points Muscle glycogen and intramuscular triglycerides (IMTG, stored in lipid droplets) are important energy substrates during prolonged exercise. Exercise‐induced changes in lipid droplet (LD) morphology (i.e. LD size and number) have…
View article: Lamin-Related Congenital Muscular Dystrophy Alters Mechanical Signaling and Skeletal Muscle Growth
Lamin-Related Congenital Muscular Dystrophy Alters Mechanical Signaling and Skeletal Muscle Growth Open
Laminopathies are a clinically heterogeneous group of disorders caused by mutations in the LMNA gene, which encodes the nuclear envelope proteins lamins A and C. The most frequent diseases associated with LMNA mutations are characterized b…
View article: Graded reductions in pre‐exercise glycogen concentration do not augment exercise‐induced nuclear AMPK and PGC‐1α protein content in human muscle
Graded reductions in pre‐exercise glycogen concentration do not augment exercise‐induced nuclear AMPK and PGC‐1α protein content in human muscle Open
New Findings What is the central question of this study? What is the absolute level of pre‐exercise glycogen concentration required to augment the exercise‐induced signalling response regulating mitochondrial biogenesis? What is the main f…
View article: Achieving energy balance with a high‐fat meal does not enhance skeletal muscle adaptation and impairs glycaemic response in a sleep‐low training model
Achieving energy balance with a high‐fat meal does not enhance skeletal muscle adaptation and impairs glycaemic response in a sleep‐low training model Open
New Findings What is the central question of this study? Does achieving energy balance mainly with ingested fat in a ‘sleep‐low’ model of training with low muscle glycogen affect the early training adaptive response during recovery? What i…
View article: Lamin-related congenital muscular dystrophy alters mechanical signaling and skeletal muscle growth
Lamin-related congenital muscular dystrophy alters mechanical signaling and skeletal muscle growth Open
Background Laminopathies are a clinically heterogeneous group of disorders caused by mutations in the LMNA gene, which encodes the nuclear envelope proteins lamins A and C. The most frequent diseases associated with LMNA mutations are char…
View article: Lamin Mutations Cause Increased YAP Nuclear Entry in Muscle Stem Cells
Lamin Mutations Cause Increased YAP Nuclear Entry in Muscle Stem Cells Open
Mutations in the LMNA gene, encoding the nuclear envelope A-type lamins, are responsible for muscular dystrophies, the most severe form being the LMNA-related congenital muscular dystrophy (L-CMD), with severe defects in myonucleus integri…
View article: UBR5 is a novel E3 ubiquitin ligase involved in skeletal muscle hypertrophy and recovery from atrophy
UBR5 is a novel E3 ubiquitin ligase involved in skeletal muscle hypertrophy and recovery from atrophy Open
Key points We have recently identified that a HECT domain E3 ubiquitin ligase, named UBR5, is altered epigenetically (via DNA methylation) after human skeletal muscle hypertrophy, where its gene expression is positively correlated with inc…
View article: Exercise‐induced muscle damage: What is it, what causes it and what are the nutritional solutions?
Exercise‐induced muscle damage: What is it, what causes it and what are the nutritional solutions? Open
Exercise‐induced muscle damage (EIMD) is characterized by symptoms that present both immediately and for up to 14 days after the initial exercise bout. The main consequence of EIMD for the athlete is the loss of skeletal muscle function an…
View article: microRNA-184 and Its lncRNA Sponge UCA1 are Induced in Wounded Keratinocytes in a Store-Operated Calcium Entry-Dependent Manner
microRNA-184 and Its lncRNA Sponge UCA1 are Induced in Wounded Keratinocytes in a Store-Operated Calcium Entry-Dependent Manner Open
Emerging evidence implicates microRNAs (miRNA) in the regulation of keratinocyte migration. However, the putative roles of microRNA-184 (miR-184) in keratinocyte migration have not been examined. Here, we show that miR-184 expression was e…
View article: Vitamin D and the Athlete: Current Perspectives and New Challenges
Vitamin D and the Athlete: Current Perspectives and New Challenges Open