Daniel J. Slade
YOU?
Author Swipe
View article: Fusobacterium nucleatum infection modulates the transcriptome and epigenome of HCT116 colorectal cancer cells in an oxygen-dependent manner
Fusobacterium nucleatum infection modulates the transcriptome and epigenome of HCT116 colorectal cancer cells in an oxygen-dependent manner Open
Fusobacterium nucleatum , a gram-negative oral bacterium, has been consistently validated as a strong contributor to the progression of several types of cancer, including colorectal (CRC) and pancreatic cancer. While previous in vitro stud…
View article: Spatial transcriptomic analysis reveals local effects of intratumoral fusobacterial infection on DNA damage and immune signaling in rectal cancer
Spatial transcriptomic analysis reveals local effects of intratumoral fusobacterial infection on DNA damage and immune signaling in rectal cancer Open
Mucinous colorectal cancer (CRC) is a common histological subtype of colorectal adenocarcinoma, associated with a poor response to chemoradiotherapy. The commensal facultative anaerobes fusobacteria, have been associated with poor prognosi…
View article: Type 4 pili mediated natural competence in<i>Fusobacterium nucleatum</i>
Type 4 pili mediated natural competence in<i>Fusobacterium nucleatum</i> Open
Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characteriza…
View article: Patients with Increased Levels of Fusobacterium Tumoral Abundance are Associated with Better Outcomes in Mucinous Colorectal Cancer.
Patients with Increased Levels of Fusobacterium Tumoral Abundance are Associated with Better Outcomes in Mucinous Colorectal Cancer. Open
There is currently an urgent need to identify factors predictive of immunogenicity in colorectal cancer (CRC). Mucinous CRC is a distinct histological subtype of CRC, associated with a poor response to chemotherapy. Recent evidence suggest…
View article: Enhanced Fusobacterium nucleatum Genetics Using Host DNA Methyltransferases To Bypass Restriction-Modification Systems
Enhanced Fusobacterium nucleatum Genetics Using Host DNA Methyltransferases To Bypass Restriction-Modification Systems Open
Fusobacterium nucleatum is an oral opportunistic pathogen associated with diseases that include cancer and preterm birth. Our understanding of how this bacterium modulates human disease has been hindered by a lack of genetic systems.
View article: <i>Fusobacterium nucleatum</i> induces proliferation and migration in pancreatic cancer cells through host autocrine and paracrine signaling
<i>Fusobacterium nucleatum</i> induces proliferation and migration in pancreatic cancer cells through host autocrine and paracrine signaling Open
The tumor microbiome is increasingly implicated in cancer progression and resistance to chemotherapy. In pancreatic ductal adenocarcinoma (PDAC), high intratumoral loads of Fusobacterium nucleatum correlate with shorter survival in patient…
View article: DNA methyltransferase enhanced <i>Fusobacterium nucleatum</i> genetics
DNA methyltransferase enhanced <i>Fusobacterium nucleatum</i> genetics Open
Bacterial restriction-modification (R-M) systems are a first line immune defense against foreign DNA from viruses and other bacteria. While R-M systems are critical in maintaining genome integrity, R-M nucleases unfortunately present signi…
View article: The <i>Shigella</i> Spp. Type III Effector Protein OspB Is a Cysteine Protease
The <i>Shigella</i> Spp. Type III Effector Protein OspB Is a Cysteine Protease Open
Shigella spp. are important human pathogens and among the leading causes of diarrheal mortality worldwide, especially in children. Virulence depends on the Shigella type III secretion system (T3SS).
View article: Mucosal microbiomes and <i>Fusobacterium</i> genomics in Vietnamese colorectal cancer patients
Mucosal microbiomes and <i>Fusobacterium</i> genomics in Vietnamese colorectal cancer patients Open
Perturbations in the gut microbiome have been linked to the promotion and prognosis of colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite the increasing health bu…
View article: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models
Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models Open
Colon cancer is a leading cause of cancer morbidity and mortality, and it is hypothesized that dysbiosis in the gut microbiota contributes to colon tumorigenesis. Fusobacterium nucleatum , a member of the oropharyngeal microbiome, is enric…
View article: CRISPR-Cas systems in <i>Fusobacterium</i>: An untapped genetic frontier
CRISPR-Cas systems in <i>Fusobacterium</i>: An untapped genetic frontier Open
Fusobacterium nucleatum has recently received significant attention for its strong connection with the acceleration and gravity of multiple cancers (e.g., colorectal, pancreatic, esophageal). However, our understanding of the molecular mec…
View article: The <i>Shigella</i> type III effector protein OspB is a cysteine protease
The <i>Shigella</i> type III effector protein OspB is a cysteine protease Open
The type III secretion system is required for virulence of many pathogenic bacteria. Bacterial effector proteins delivered into target host cells by this system modulate host signaling pathways and processes in a manner that promotes infec…
View article: CEACAM1 Activation by CbpF-Expressing E. coli
CEACAM1 Activation by CbpF-Expressing E. coli Open
Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the …
View article: Fusobacterium nucleatum CbpF Mediates Inhibition of T Cell Function Through CEACAM1 Activation
Fusobacterium nucleatum CbpF Mediates Inhibition of T Cell Function Through CEACAM1 Activation Open
F. nucleatum is an anaerobic bacterium that is associated with several tumor entities and promotes tumorigenesis. Recent evidence suggests that F. nucleatum binds the inhibitory receptor carcinoembryonic antigen cell adhesion molecule 1 (C…
View article: Genome Sequences for Two Acinetobacter baumannii Strains Obtained Using the Unicycler Hybrid Assembly Pipeline
Genome Sequences for Two Acinetobacter baumannii Strains Obtained Using the Unicycler Hybrid Assembly Pipeline Open
Here, we report a complete genome sequence for Acinetobacter baumannii strain ATCC 17961, with plasmid sequences, and a high-quality (>98% complete) build for A. baumannii strain AB09-003. These genome sequences were generated by combining…
View article: <i>Fusobacterium nucleatum</i> host-cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration
<i>Fusobacterium nucleatum</i> host-cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration Open
The progression of inflammation-associated colon cancer is driven by resident bacteria.
View article: <i>Fusobacterium nucleatum</i>host cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration
<i>Fusobacterium nucleatum</i>host cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration Open
Fusobacterium nucleatum is implicated in the acceleration of colorectal cancer (CRC), yet the mechanisms by which this bacterium modulates the tumor microenvironment remain understudied. Here we show that binding and cellular invasion of C…
View article: Utilizing Whole <i>Fusobacterium</i> Genomes To Identify, Correct, and Characterize Potential Virulence Protein Families
Utilizing Whole <i>Fusobacterium</i> Genomes To Identify, Correct, and Characterize Potential Virulence Protein Families Open
Fusobacterium spp. are emerging pathogens that contribute to mammalian and human diseases, including colorectal cancer. Despite a validated connection with disease, few proteins have been characterized that define a direct molecular mechan…
View article: Comparison of type 5d autotransporter phospholipases demonstrates a correlation between high activity and intracellular pathogenic lifestyle
Comparison of type 5d autotransporter phospholipases demonstrates a correlation between high activity and intracellular pathogenic lifestyle Open
Autotransporters, or type 5 secretion systems, are widespread surface proteins of Gram-negative bacteria often associated with virulence functions. Autotransporters consist of an outer membrane β-barrel domain and an exported passenger. In…
View article: Calcium Regulates the Nuclear Localization of Protein Arginine Deiminase 2
Calcium Regulates the Nuclear Localization of Protein Arginine Deiminase 2 Open
Protein arginine deiminases (PADs) are calcium-dependent enzymes that mediate the post-translational conversion of arginine into citrulline. Dysregulated PAD activity is associated with numerous autoimmune disorders and cancers. In breast …
View article: Cyclic di-nucleotides – what is their role in biofilm formation and pathogenicity of Fusobacterium nucleatum?
Cyclic di-nucleotides – what is their role in biofilm formation and pathogenicity of Fusobacterium nucleatum? Open
Introduction Cyclic di-nucleotides (CDNs) act as important second messengers in bacteria, regulating multiple cellular functions, including biofilm formation. Fusobacterium nucleatum is a key player in disease-associated biofilms in period…
View article: Complete Genome Sequence of Fusobacterium necrophorum subsp. <i>necrophorum</i> ATCC 25286
Complete Genome Sequence of Fusobacterium necrophorum subsp. <i>necrophorum</i> ATCC 25286 Open
Fusobacterium necrophorum is a pathogenic Gram-negative, anaerobic bacterium. In this study, we present the first complete genome sequence of Fusobacterium necrophorum subsp.
View article: Reevaluating the <i>Fusobacterium</i> Virulence Factor Landscape
Reevaluating the <i>Fusobacterium</i> Virulence Factor Landscape Open
Fusobacteríum are Gram-negative, anaerobic, opportunistic pathogens involved in multiple diseases, including the oral pathogen Fusobacterium nucleatum being linked to the progression and severity of colorectal cancer. The global identifica…