Explanipedia

Advancing inflammasome research: No-wash immunoassays for active IL-18 and gasdermin D-mediated pyroptosis detection 3596 Open

Martha A. O’Brien, Daniel Mamott, James J. Cali, Kim Haupt, Dan Lazar · 2025

Description Effective tools for monitoring inflammasome activation are crucial for understanding its role in immune function and advancing drug discovery efforts. We previously developed high throughput-amenable assays for caspase-1 activa…

A High-Quality Blue Whale Genome, Segmental Duplications, and Historical Demography Open

Yury V. Bukhman, Phillip A. Morin, Susanne Meyer, Li‐Fang Chu, Jeff K. Jacobsen , et al. · 2024

Biology History Sociology

The blue whale, Balaenoptera musculus, is the largest animal known to have ever existed, making it an important case study in longevity and resistance to cancer. To further this and other blue whale-related research, we report a reference-…

Chromosome level genome assembly of the Etruscan shrew Suncus etruscus Open

Yury V. Bukhman, Susanne Meyer, Li‐Fang Chu, Linelle Abueg, Jessica Antosiewicz‐Bourget , et al. · 2024

Biology

Suncus etruscus is one of the world’s smallest mammals, with an average body mass of about 2 grams. The Etruscan shrew’s small body is accompanied by a very high energy demand and numerous metabolic adaptations. Here we report a chromosome…

Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing Open

J. Vanessa Conrad, Susanne Meyer, Pranav S. Ramesh, Jaime A. Neira, Margaret Rusteika , et al. · 2023

Biology Medicine

Sus scrofa domesticus (pig) has served as a superb large mammalian model for biomedical studies because of its comparable physiology and organ size to humans. The derivation of transgene-free porcine induced pluripotent stem cells (PiPSCs)…

Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays Open

Kelly A. Teske, Cesear Corona, Jennifer Wilkinson, Daniel Mamott, David A. Good , et al. · 2023

Biology Chemistry

As a key regulator of the innate immune system, the NLRP3 inflammasome responds to a variety of environmental insults through activation of caspase-1 and release of the proinflammatory cytokines IL-1β and IL-18. Aberrant NLRP3 inflammasome…

Development of a specific, active human IL-18 assay for assessing inflammasome activation Open

Daniel Mamott, Sarah D. Mahan, Jim Cali, Dan Lazar, Martha A. O’Brien · 2023

Biology Chemistry

Interleukin-18 (IL-18) was first identified for its ability to induce interferon-γ expression. IL-18 is a proinflammatory cytokine and its release along with IL-1β is a primary output of inflammasome activation. We have developed convenien…

A high-quality blue whale genome, segmental duplications, and historical demography Open

Yury V. Bukhman, Phillip A. Morin, Susanne Meyer, Li‐Fang Chu, Jeff K. Jacobsen , et al. · 2022

Biology

BackgroundThe blue whale, Balaenoptera musculus , is the largest animal known to have ever existed. Body size is tightly coupled to cell metabolism and environmental adaptations. A high-quality genome assembly of this magnificent animal wi…

Engineered Perineural Vascular Plexus for Modeling Developmental Toxicity Open

Gaurav Kaushik, Kartik Gupta, Victoria Harms, Elizabeth Torr, Jonathan Evans , et al. · 2020

Biology

There is a vital need to develop in vitro models of the developing human brain to recapitulate the biological effects that toxic compounds have on the brain. To model perineural vascular plexus (PNVP) in vitro, which is a key stage in embr…

Additional file 2: Table S1. of Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm Open