Daniel Murante
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View article: Drivers of diversification in fungal pathogen populations
Drivers of diversification in fungal pathogen populations Open
To manage and treat chronic fungal diseases effectively, we require an improved understanding of their complexity. There is an increasing appreciation that chronic infection populations are often heterogeneous due to diversification and dr…
View article: Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung
Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung Open
The genetic disease cystic fibrosis (CF) frequently leads to chronic lung infections by bacteria and fungi. We identified three individuals with CF with persistent lung infections dominated by Clavispora ( Candida ) lusitaniae . Whole-geno…
View article: In host evolution of <i>Exophiala dermatitidis</i> in cystic fibrosis lung micro-environment
In host evolution of <i>Exophiala dermatitidis</i> in cystic fibrosis lung micro-environment Open
Individuals with cystic fibrosis (CF) are susceptible to chronic lung infections that lead to inflammation and irreversible lung damage. While most respiratory infections that occur in CF are caused by bacteria, some are dominated by fungi…
View article: Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung
Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung Open
The genetic disease cystic fibrosis (CF) frequently leads to chronic lung infections by bacteria and fungi. We identified three individuals with CF with persistent lung infections dominated by Clavispora ( Candida ) lusitaniae . Whole geno…
View article: In host evolution of <i>Exophiala dermatitidis</i> in cystic fibrosis lung micro-environment
In host evolution of <i>Exophiala dermatitidis</i> in cystic fibrosis lung micro-environment Open
Individuals with cystic fibrosis (CF) are susceptible to chronic lung infections that lead to inflammation and irreversible lung damage. While most respiratory infections that occur in CF are caused by bacteria, some are dominated by fungi…
View article: Loss-of-Function <i>ROX1</i> Mutations Suppress the Fluconazole Susceptibility of <i>upc2A</i> Δ Mutation in Candida glabrata, Implicating Additional Positive Regulators of Ergosterol Biosynthesis
Loss-of-Function <i>ROX1</i> Mutations Suppress the Fluconazole Susceptibility of <i>upc2A</i> Δ Mutation in Candida glabrata, Implicating Additional Positive Regulators of Ergosterol Biosynthesis Open
Candida glabrata is one of the most important human fungal pathogens and has reduced susceptibility to azole-class inhibitors of ergosterol biosynthesis. Although ergosterol is the target of two of the three classes of antifungal drugs, re…
View article: Loss-of-function <i>ROX1</i> mutations suppress the fluconazole susceptibility of <i>upc2A</i>Δ mutation in <i>Candida glabrata</i>, implicating additional positive regulators of ergosterol biosynthesis
Loss-of-function <i>ROX1</i> mutations suppress the fluconazole susceptibility of <i>upc2A</i>Δ mutation in <i>Candida glabrata</i>, implicating additional positive regulators of ergosterol biosynthesis Open
Two of the major classes of antifungal drugs in clinical use target ergosterol biosynthesis. Despite its importance, our understanding of the transcriptional regulation of ergosterol biosynthesis genes in pathogenic fungi is essentially li…
View article: New Mitochondrial Targets in Fungal Pathogens
New Mitochondrial Targets in Fungal Pathogens Open
In eukaryotic cells, mitochondria are responsible for the synthesis of ATP using power generated by the electron transport chain (ETC). While much of what is known about mitochondria has been gained from a study of a small number of model …
View article: Systematic Complex Haploinsufficiency-Based Genetic Analysis of<i>Candida albicans</i>Transcription Factors: Tools and Applications to Virulence-Associated Phenotypes
Systematic Complex Haploinsufficiency-Based Genetic Analysis of<i>Candida albicans</i>Transcription Factors: Tools and Applications to Virulence-Associated Phenotypes Open
Genetic interaction analysis is a powerful approach to the study of complex biological processes that are dependent on multiple genes. Because of the largely diploid nature of the human fungal pathogen Candida albicans, genetic interaction…
View article: The biofilm TF network contains feedforward loops that regulate <i>BCR1</i> expression.
The biofilm TF network contains feedforward loops that regulate <i>BCR1</i> expression. Open
(A) A summary of the phenotypic genetic interaction network for the biofilm TFs. (B) Three network modules that show both phenotypic interactions and downstream target expression changes consistent feedforward loops. The numbers in parenth…
View article: The <i>ndt80</i>Δ/<i>NDT80</i> and <i>bcr1</i>Δ/<i>BCR1</i> show altered biofilm architecture.
The <i>ndt80</i>Δ/<i>NDT80</i> and <i>bcr1</i>Δ/<i>BCR1</i> show altered biofilm architecture. Open
Confocal microscopy images of wild type, ndt80Δ/NDT80, bcr1Δ/BCR1, tec1Δ/TEC1, and ndt80Δ/NDT80 tec1Δ/TEC1 strains grown in YETS for 48 hr. Cells were stained with concanavalin A. See Materials and methods for full details. The top image i…
View article: Heterozygous deletion mutants of six core biofilm transcription factors show simple and complex hapoinsufficiency for biofilm density.
Heterozygous deletion mutants of six core biofilm transcription factors show simple and complex hapoinsufficiency for biofilm density. Open
(A) The density of the biofilms formed by the indicated wild type (SN250), heterozygous and homozygous deletion strains was determined by measuring the OD600 after incubation for 48 hr as described in Materials and Methods (YETS medium). T…
View article: Epistasis analysis of the biofilm density for the biofilm TF network.
Epistasis analysis of the biofilm density for the biofilm TF network. Open
(A) The nomarlized biofilm density (NBD) for each double heterozygote mutant was determined after 48 hr biofilm formation as described in Materials and Methods. The predicted NBD for each double heterozygous mutant was calculated by multip…
View article: The altered architecture of <i>ndt80</i>Δ/<i>NDT80</i> is strongly dependent on Rob1 and Tec1.
The altered architecture of <i>ndt80</i>Δ/<i>NDT80</i> is strongly dependent on Rob1 and Tec1. Open
(A) Complementation of ndt80Δ/NDT80 with an allele under endogenous promoter restores wild type levels of normalized biofilm density (NBD) NBD=OD600 mutant/OD600 wild type. The blue bar indicates statistically significant increase relative…
View article: Genetic analysis of the Candida albicans biofilm transcription factor network using simple and complex haploinsufficiency
Genetic analysis of the Candida albicans biofilm transcription factor network using simple and complex haploinsufficiency Open
Biofilm formation by Candida albicans is a key aspect of its pathobiology and is regulated by an integrated network of transcription factors (Bcr1, Brg1, Efg1, Ndt80, Rob1, and Tec1). To understand the details of how the transcription fact…