Daniel Neiman
YOU?
Author Swipe
View article: Genetic/epigenetic DNA markers for linking suspects and tissues in complex crime scenes
Genetic/epigenetic DNA markers for linking suspects and tissues in complex crime scenes Open
Analysis of DNA found at a crime scene can provide crucial information on the identity of the individual who has left the DNA, as well as the tissue origin of the DNA. However, the current methods used for DNA profiling and for the identif…
View article: Circulating eosinophil cell-free DNA as a noninvasive biomarker for perforated appendicitis in pediatric patients-a proof-of-concept study
Circulating eosinophil cell-free DNA as a noninvasive biomarker for perforated appendicitis in pediatric patients-a proof-of-concept study Open
View article: DNA methylation-based assessment of cell composition in human pancreas and islets
DNA methylation-based assessment of cell composition in human pancreas and islets Open
Assessment of pancreas cell type composition is crucial to the understanding of the genesis of diabetes. Current approaches use immunodetection of protein markers, for example insulin as a marker of beta-cells. A major limitation of these …
View article: DNA methylation-based assessment of cell composition in human pancreas and islets
DNA methylation-based assessment of cell composition in human pancreas and islets Open
Assessment of pancreas cell type composition is crucial to the understanding of the genesis of diabetes. Current approaches use immunodetection of protein markers, for example insulin as a marker of beta-cells. A major limitation of these …
View article: DNA methylation-based assessment of cell composition in human pancreas and islets
DNA methylation-based assessment of cell composition in human pancreas and islets Open
Assessment of pancreas cell type composition is crucial to the understanding of the genesis of diabetes. Current approaches use immunodetection of protein markers, for example insulin as a marker of beta-cells. A major limitation of these …
View article: DNA Methylation–Based Assessment of Cell Composition in Human Pancreas and Islets
DNA Methylation–Based Assessment of Cell Composition in Human Pancreas and Islets Open
Assessment of pancreas cell type composition is crucial to the understanding of the genesis of diabetes. Current approaches use immunodetection of protein markers, for example, insulin as a marker of β-cells. A major limitation of these me…
View article: Protein biomarkers and alternatively methylated cell-free DNA detect early stage pancreatic cancer
Protein biomarkers and alternatively methylated cell-free DNA detect early stage pancreatic cancer Open
Objective Pancreatic ductal adenocarcinoma (PDAC) is commonly diagnosed at an advanced stage. Liquid biopsy approaches may facilitate detection of early stage PDAC when curative treatments can be employed. Design To assess circulating mark…
View article: Chronic graft-versus-host disease detected by tissue-specific cell-free DNA methylation biomarkers
Chronic graft-versus-host disease detected by tissue-specific cell-free DNA methylation biomarkers Open
This work was supported by grants from the Ernest and Bonnie Beutler Research Program of Excellence in Genomic Medicine, The Israel Science Foundation, the Waldholtz/Pakula family, the Robert M. and Marilyn Sternberg Family Charitable Foun…
View article: A method for cleaning of used disposable plastic parts from automated <scp>RNA</scp> extraction system
A method for cleaning of used disposable plastic parts from automated <span>RNA</span> extraction system Open
During the first wave of COVID‐19 worldwide pandemic, the number of testing in Israel was severely affected by the shortage of reagents and consumables required to operate the instrument performing the sample extraction before the reverse …
View article: Beta‐cell death and dysfunction drives hyperglycaemia in organ donors
Beta‐cell death and dysfunction drives hyperglycaemia in organ donors Open
Background Donor hyperglycaemia following brain death has been attributed to reversible insulin resistance. However, our islet and pancreas transplant data suggest that other mechanisms may be predominant. We aimed to determine the relatio…
View article: P.083 Liquid biopsies reveal brain cell death in central nervous system tumors
P.083 Liquid biopsies reveal brain cell death in central nervous system tumors Open
Background: Circulating cell-free DNA (cfDNA) is a novel type of biomarker with a broad utility in diagnostic medicine, based on the release of DNA fragments from dying cells to the circulation. We developed an approach for identifying the…
View article: A universal cell‐free <scp>DNA</scp> approach for response prediction to preoperative chemoradiation in rectal cancer
A universal cell‐free <span>DNA</span> approach for response prediction to preoperative chemoradiation in rectal cancer Open
The standard treatment approach for stage II/III rectal cancer is neoadjuvant chemoradiation therapy (nCRT) followed by surgery. In recent years, new treatment approaches have led to higher rates of complete tumor eradication combined with…
View article: Elevated brain-derived cell-free DNA among patients with first psychotic episode – a proof-of-concept study
Elevated brain-derived cell-free DNA among patients with first psychotic episode – a proof-of-concept study Open
Schizophrenia is a common, severe, and debilitating psychiatric disorder. Despite extensive research there is as yet no biological marker that can aid in its diagnosis and course prediction. This precludes early detection and intervention.…
View article: B cell-derived cfDNA after primary BNT162b2 mRNA vaccination anticipates memory B cells and SARS-CoV-2 neutralizing antibodies
B cell-derived cfDNA after primary BNT162b2 mRNA vaccination anticipates memory B cells and SARS-CoV-2 neutralizing antibodies Open
View article: Author response: Elevated brain-derived cell-free DNA among patients with first psychotic episode – a proof-of-concept study
Author response: Elevated brain-derived cell-free DNA among patients with first psychotic episode – a proof-of-concept study Open
View article: Liquid biopsy reveals collateral tissue damage in cancer
Liquid biopsy reveals collateral tissue damage in cancer Open
Cancer inflicts damage to surrounding normal tissues, which can culminate in fatal organ failure. Here, we demonstrate that cell death in organs affected by cancer can be detected by tissue-specific methylation patterns of circulating cell…
View article: Author Correction: ChIP-seq of plasma cell-free nucleosomes identifies gene expression programs of the cells of origin
Author Correction: ChIP-seq of plasma cell-free nucleosomes identifies gene expression programs of the cells of origin Open
View article: Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests
Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests Open
Adaptive sample pooling for SARS-CoV-2 testing based on positivity rates spares 76% of test reactions without compromising specificity or sensitivity.
View article: ChIP-seq of plasma cell-free nucleosomes identifies gene expression programs of the cells of origin
ChIP-seq of plasma cell-free nucleosomes identifies gene expression programs of the cells of origin Open
View article: Multiplexing DNA methylation markers to detect circulating cell-free DNA derived from human pancreatic β cells
Multiplexing DNA methylation markers to detect circulating cell-free DNA derived from human pancreatic β cells Open
It has been proposed that unmethylated insulin promoter fragments in plasma derive exclusively from β cells, reflect their recent demise, and can be used to assess β cell damage in type 1 diabetes. Herein we describe an ultrasensitive assa…
View article: Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection
Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection Open
View article: Circulating Unmethylated Insulin DNA As a Biomarker of Human Beta Cell Death: A Multi-laboratory Assay Comparison
Circulating Unmethylated Insulin DNA As a Biomarker of Human Beta Cell Death: A Multi-laboratory Assay Comparison Open
Context There is an unmet need for biomarkers of pancreatic beta-cell death to improve early diagnosis of type 1 diabetes, enroll subjects into clinical trials, and assess treatment response. To address this need, several groups developed …
View article: ChIP-seq of plasma cell-free nucleosomes identifies cell-of-origin gene expression programs
ChIP-seq of plasma cell-free nucleosomes identifies cell-of-origin gene expression programs Open
Genomic DNA is packed by histone proteins that carry a multitude of post-translational modifications that reflect cellular transcriptional state. Cell-free DNA (cfDNA) is derived from fragmented chromatin in dying cells, and as such it ret…
View article: Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease
Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease Open
View article: Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease
Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease Open
Methylation patterns of circulating cell-free DNA (cfDNA) contain rich information about recent cell death events in the body. Here, we present an approach for unbiased determination of the tissue origins of cfDNA, using a reference methyl…
View article: Specific detection of cell-free DNA derived from intestinal epithelial cells using methylation patterns
Specific detection of cell-free DNA derived from intestinal epithelial cells using methylation patterns Open
Epithelial cells of the intestine undergo rapid turnover and are thought to be cleared via stool. Disruption of tissue architecture, as occurs in colorectal cancer (CRC), results in the release of material from dying intestinal epithelial …
View article: Monitoring liver damage using hepatocyte-specific methylation markers in cell-free circulating DNA
Monitoring liver damage using hepatocyte-specific methylation markers in cell-free circulating DNA Open
Liver damage is typically inferred from serum measurements of cytoplasmic liver enzymes. DNA molecules released from dying hepatocytes are an alternative biomarker, unexplored so far, potentially allowing for quantitative assessment of liv…
View article: Non-invasive detection of human cardiomyocyte death using methylation patterns of circulating DNA
Non-invasive detection of human cardiomyocyte death using methylation patterns of circulating DNA Open
Detection of cardiomyocyte death is crucial for the diagnosis and treatment of heart disease. Here we use comparative methylome analysis to identify genomic loci that are unmethylated specifically in cardiomyocytes, and develop these as bi…
View article: Beta Cell Death by Cell-free DNA and Outcome After Clinical Islet Transplantation
Beta Cell Death by Cell-free DNA and Outcome After Clinical Islet Transplantation Open
cfDNA-based estimation of beta cell death 24 hours after islet allotransplantation correlates with clinical outcome and could predict early engraftment.
View article: Islet cells share promoter hypomethylation independently of expression, but exhibit cell-type–specific methylation in enhancers
Islet cells share promoter hypomethylation independently of expression, but exhibit cell-type–specific methylation in enhancers Open
Significance We have studied the dynamics of DNA methylation in pancreatic α- and β-cells and reached surprising insights into the establishment of islet cell identity. Different islet cell types share lack of methylation in cell-type–spec…