Daniel R. Zollinger
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View article: Analytical validation of a novel comprehensive genomic profiling informed circulating tumor DNA monitoring assay for solid tumors
Analytical validation of a novel comprehensive genomic profiling informed circulating tumor DNA monitoring assay for solid tumors Open
Emerging technologies focused on the detection and quantification of circulating tumor DNA (ctDNA) in blood show extensive potential for managing patient treatment decisions, informing risk of recurrence, and predicting response to therapy…
View article: Organ-specific immunity: A tissue analysis framework for investigating local immune responses to SARS-CoV-2
Organ-specific immunity: A tissue analysis framework for investigating local immune responses to SARS-CoV-2 Open
Local immune activation at mucosal surfaces, mediated by mucosal lymphoid tissues, is vital for effective immune responses against pathogens. While pathogens like severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can spread to m…
View article: Molecular residual disease detection in resected, muscle-invasive urothelial cancer with a tissue-based comprehensive genomic profiling–informed personalized monitoring assay
Molecular residual disease detection in resected, muscle-invasive urothelial cancer with a tissue-based comprehensive genomic profiling–informed personalized monitoring assay Open
Introduction Circulating tumor DNA (ctDNA) detection postoperatively may identify patients with urothelial cancer at a high risk of relapse. Pragmatic tools building off clinical tumor next-generation sequencing (NGS) platforms could have …
View article: Comprehensive Genomic Profiling (CGP)-Informed Personalized Molecular Residual Disease (MRD) Detection: An Exploratory Analysis from the PREDATOR Study of Metastatic Colorectal Cancer (mCRC) Patients Undergoing Surgical Resection
Comprehensive Genomic Profiling (CGP)-Informed Personalized Molecular Residual Disease (MRD) Detection: An Exploratory Analysis from the PREDATOR Study of Metastatic Colorectal Cancer (mCRC) Patients Undergoing Surgical Resection Open
A majority of patients with metastatic colorectal cancer (mCRC) experience recurrence post curative-intent surgery. The addition of adjuvant chemotherapy has shown to provide limited survival benefits when applied to all patients. Therefor…
View article: Spatially resolved whole transcriptome profiling in human and mouse tissue using Digital Spatial Profiling
Spatially resolved whole transcriptome profiling in human and mouse tissue using Digital Spatial Profiling Open
Emerging spatial profiling technology has enabled high-plex molecular profiling in biological tissues, preserving the spatial and morphological context of gene expression. Here, we describe expanding the chemistry for the Digital Spatial P…
View article: Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy
Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy Open
View article: Single-nucleus and spatial transcriptome profiling of pancreatic cancer identifies multicellular dynamics associated with neoadjuvant treatment
Single-nucleus and spatial transcriptome profiling of pancreatic cancer identifies multicellular dynamics associated with neoadjuvant treatment Open
View article: Spatially resolved whole transcriptome profiling in human and mouse tissue using Digital Spatial Profiling
Spatially resolved whole transcriptome profiling in human and mouse tissue using Digital Spatial Profiling Open
Emerging spatial profiling technology has enabled high-plex molecular profiling in biological tissues, preserving the spatial and morphological context of gene expression. Here we describe expanding the chemistry for the Digital Spatial Pr…
View article: Spatially organized multicellular immune hubs in human colorectal cancer
Spatially organized multicellular immune hubs in human colorectal cancer Open
View article: COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets
COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets Open
View article: A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2
A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 Open
The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and acute respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue…
View article: Multicellular immune hubs and their organization in MMRd and MMRp colorectal cancer
Multicellular immune hubs and their organization in MMRd and MMRp colorectal cancer Open
Immune responses to cancer are highly variable, with mismatch repair-deficient (MMRd) tumors exhibiting more anti-tumor immunity than mismatch repair-proficient (MMRp) tumors. To understand the rules governing these varied responses, we tr…
View article: Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma
Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma Open
View article: Single-nucleus and spatial transcriptomics of archival pancreatic cancer reveals multi-compartment reprogramming after neoadjuvant treatment
Single-nucleus and spatial transcriptomics of archival pancreatic cancer reveals multi-compartment reprogramming after neoadjuvant treatment Open
Pancreatic ductal adenocarcinoma (PDAC) remains a treatment-refractory disease. Characterizing PDAC by mRNA profiling remains particularly challenging. Previously identified bulk expression subtypes were influenced by contaminating stroma …
View article: Opposing immune and genetic forces shape oncogenic programs in synovial sarcoma
Opposing immune and genetic forces shape oncogenic programs in synovial sarcoma Open
Synovial sarcoma is an aggressive mesenchymal neoplasm, driven by the SS18-SSX fusion, and characterized by immunogenic antigens expression and exceptionally low T cell infiltration levels. To study the cancer-immune interplay in this dise…
View article: High multiplex, digital spatial profiling of proteins and RNA in fixed tissue using genomic detection methods
High multiplex, digital spatial profiling of proteins and RNA in fixed tissue using genomic detection methods Open
We have developed Digital Spatial Profiling (DSP), a non-destructive method for high-plex spatial profiling of proteins and RNA, using oligonucleotide detection technologies with unlimited multiplexing capability. The key breakthroughs und…
View article: Glial βII Spectrin Contributes to Paranode Formation and Maintenance
Glial βII Spectrin Contributes to Paranode Formation and Maintenance Open
Action potential conduction along myelinated axons depends on high densities of voltage-gated Na+ channels at the nodes of Ranvier. Flanking each node, paranodal junctions (paranodes) are formed between axons and Schwann cells i…
View article: Clemastine rescues myelination defects and promotes functional recovery in hypoxic brain injury
Clemastine rescues myelination defects and promotes functional recovery in hypoxic brain injury Open
Hypoxia can injure brain white matter tracts, comprised of axons and myelinating oligodendrocytes, leading to cerebral palsy in neonates and delayed post-hypoxic leukoencephalopathy (DPHL) in adults. In these conditions, white matter injur…
View article: An αII Spectrin-Based Cytoskeleton Protects Large-Diameter Myelinated Axons from Degeneration
An αII Spectrin-Based Cytoskeleton Protects Large-Diameter Myelinated Axons from Degeneration Open
Axons must withstand mechanical forces, including tension, torsion, and compression. Spectrins and actin form a periodic cytoskeleton proposed to protect axons against these forces. However, because spectrins also participate in assembly o…
View article: The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier
The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier Open
A high density of Na+ channels at nodes of Ranvier is necessary for rapid and efficient action potential propagation in myelinated axons. Na+ channel clustering is thought to depend on two axonal cell adhesion molecules that mediate intera…
View article: Author response: The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier
Author response: The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier Open
Full text Figures and data Side by side Abstract Introduction Results Discussion Materials and methods References Decision letter Author response Article and author information Metrics Abstract A high density of Na+ channels at nodes of Ra…
View article: The Polarity Protein Pals1 Regulates Radial Sorting of Axons
The Polarity Protein Pals1 Regulates Radial Sorting of Axons Open
Myelin is essential for rapid and efficient action potential propagation in vertebrates. However, the molecular mechanisms regulating myelination remain incompletely characterized. For example, even before myelination begins in the PNS, Sc…
View article: BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells
BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells Open
In myelinated axons, K + channels are clustered in distinct membrane domains to regulate action potentials (APs). At nodes of Ranvier, Kv7 channels are expressed with Na + channels, whereas Kv1 channels flank nodes at juxtaparanodes. Regul…
View article: Daam2-PIP5K Is a Regulatory Pathway for Wnt Signaling and Therapeutic Target for Remyelination in the CNS
Daam2-PIP5K Is a Regulatory Pathway for Wnt Signaling and Therapeutic Target for Remyelination in the CNS Open