Daniel Reep
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View article: iGABASnFR2: Improved genetically encoded protein sensors of GABA
iGABASnFR2: Improved genetically encoded protein sensors of GABA Open
Monitoring GABAergic inhibition in the nervous system has been enabled by development of an intensiometric molecular sensor that directly detects GABA. However, the first generation iGABASnFR exhibits low signal-to-noise and suboptimal kin…
View article: iGABASnFR2: Improved genetically encoded protein sensors of GABA
iGABASnFR2: Improved genetically encoded protein sensors of GABA Open
Monitoring GABAergic inhibition in the nervous system has been enabled by development of an intensiometric molecular sensor that directly detects GABA. However, the first generation iGABASnFR exhibits low signal-to-noise and suboptimal kin…
View article: Glutamate indicators with increased sensitivity and tailored deactivation rates
Glutamate indicators with increased sensitivity and tailored deactivation rates Open
Identifying the input-output operations of neurons requires measurements of synaptic transmission simultaneously at many of a neuron’s thousands of inputs in the intact brain. To facilitate this goal, we engineered and screened 3365 varian…
View article: Far-red fluorescent genetically encoded calcium ion indicators
Far-red fluorescent genetically encoded calcium ion indicators Open
View article: iGABASnFR2: Improved genetically encoded protein sensors of GABA
iGABASnFR2: Improved genetically encoded protein sensors of GABA Open
Monitoring GABAergic inhibition in the nervous system has been enabled by development of an intensiometric molecular sensor that directly detects GABA. However, the first generation iGABASnFR exhibits low signal-to-noise and suboptimal kin…
View article: Glutamate indicators with increased sensitivity and tailored deactivation rates
Glutamate indicators with increased sensitivity and tailored deactivation rates Open
Identifying the input-output operations of neurons requires measurements of synaptic transmission simultaneously at many of a neuron’s thousands of inputs in the intact brain. To facilitate this goal, we engineered and screened 3365 varian…
View article: Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission
Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission Open
The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit low in vivo signal-to-noise ratios, saturating activation kinetics and ex…
View article: Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator
Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator Open
View article: Fast and sensitive GCaMP calcium indicators for imaging neural populations
Fast and sensitive GCaMP calcium indicators for imaging neural populations Open
Calcium imaging with protein-based indicators 1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited b…
View article: Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission
Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission Open
The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit saturating activation kinetics and are excluded from post-synaptic densit…
View article: Fast and sensitive GCaMP calcium indicators for imaging neural populations
Fast and sensitive GCaMP calcium indicators for imaging neural populations Open
Calcium imaging with protein-based indicators is widely used to follow neural activity in intact nervous systems. The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide. These sensors report n…
View article: Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator
Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator Open
The ability to optically image cellular transmembrane voltage at millisecond-timescale resolution can offer unprecedented insight into the function of living brains in behaving animals. The chemigenetic voltage indicator Voltron is bright …
View article: Fast and sensitive GCaMP calcium indicators for imaging neural populations
Fast and sensitive GCaMP calcium indicators for imaging neural populations Open
Calcium imaging with protein-based indicators is widely used to follow neural activity in intact nervous systems. The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide. These sensors report n…
View article: A general approach to engineer positive-going eFRET voltage indicators
A general approach to engineer positive-going eFRET voltage indicators Open
Imaging membrane voltage from genetically defined cells offers the unique ability to report spatial and temporal dynamics of electrical signaling at cellular and circuit levels. Here, we present a general approach to engineer electrochromi…