Daniel T. Infield
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View article: Non-canonical Sodium Channel Isoforms Underlie Chamber Specific Cardiac Excitability
Non-canonical Sodium Channel Isoforms Underlie Chamber Specific Cardiac Excitability Open
Voltage-gated sodium (Na V ) channels drive cardiac excitability. While Na V 1.5 is the primary cardiac isoform, the composition and functional contributions of non-Na V 1.5 isoforms in the heart remain unclear. Here, we developed a chemic…
View article: Genetic Code Expansion for Mechanistic Studies in Ion Channels: An (Un)natural Union of Chemistry and Biology
Genetic Code Expansion for Mechanistic Studies in Ion Channels: An (Un)natural Union of Chemistry and Biology Open
Ion channels play central roles in biology and human health by catalyzing the transmembrane flow of electrical charge. These proteins are ideal targets for genetic code expansion (GCE) methods because it is feasible to measure ion channel …
View article: Expression and purification of fluorinated proteins from mammalian suspension culture
Expression and purification of fluorinated proteins from mammalian suspension culture Open
View article: Real-time observation of functional specialization among phosphorylation sites in CFTR
Real-time observation of functional specialization among phosphorylation sites in CFTR Open
Phosphoregulation is ubiquitous in biology. Defining the functional roles of individual phosphorylation sites within a multivalent system remains particularly challenging. We have therefore applied a chemical biology approach to light-cont…
View article: Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells
Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells Open
The aromatic side-chains of phenylalanine, tyrosine, and tryptophan interact with their environments via both hydrophobic and electrostatic interactions. Determining the extent to which these contribute to protein function and stability is…
View article: Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells
Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells Open
The aromatic side-chains of phenylalanine, tyrosine, and tryptophan interact with their environments via both hydrophobic and electrostatic interactions. Determining the extent to which these contribute to protein function and stability is…
View article: Authors’ Response: Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells
Authors’ Response: Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells Open
View article: Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells
Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells Open
The aromatic side-chains of phenylalanine, tyrosine, and tryptophan interact with their environments via both hydrophobic and electrostatic interactions. Determining the extent to which these contribute to protein function and stability is…
View article: Role of a conserved ion-binding site tyrosine in ion selectivity of the Na+/K+ pump
Role of a conserved ion-binding site tyrosine in ion selectivity of the Na+/K+ pump Open
The essential transmembrane Na+ and K+ gradients in animal cells are established by the Na+/K+ pump, a P-type ATPase that exports three Na+ and imports two K+ per ATP hydrolyzed. The mechanism by which the Na+/K+ pump distinguishes between…
View article: Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells
Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells Open
The aromatic side-chains of phenylalanine, tyrosine, and tryptophan interact with their environments via both hydrophobic and electrostatic interactions. Determining the extent to which these contribute to protein function and stability is…
View article: The molecular evolution of function in the CFTR chloride channel
The molecular evolution of function in the CFTR chloride channel Open
The ATP-binding cassette (ABC) transporter superfamily includes many proteins of clinical relevance, with genes expressed in all domains of life. Although most members use the energy of ATP binding and hydrolysis to accomplish the active i…
View article: Cation-π Interactions and their Functional Roles in Membrane Proteins
Cation-π Interactions and their Functional Roles in Membrane Proteins Open
View article: What biologists want from their chloride reporters – a conversation between chemists and biologists
What biologists want from their chloride reporters – a conversation between chemists and biologists Open
Impaired chloride transport affects diverse processes ranging from neuron excitability to water secretion, which underlie epilepsy and cystic fibrosis, respectively. The ability to image chloride fluxes with fluorescent probes has been ess…
View article: An Ancient CFTR Ortholog Informs Molecular Evolution in ABC Transporters
An Ancient CFTR Ortholog Informs Molecular Evolution in ABC Transporters Open
View article: Engineered transfer RNAs for suppression of premature termination codons
Engineered transfer RNAs for suppression of premature termination codons Open
Premature termination codons (PTCs) are responsible for 10–15% of all inherited disease. PTC suppression during translation offers a promising approach to treat a variety of genetic disorders, yet small molecules that promote PTC read-thro…
View article: Main-chain mutagenesis reveals intrahelical coupling in an ion channel voltage-sensor
Main-chain mutagenesis reveals intrahelical coupling in an ion channel voltage-sensor Open
View article: Engineered transfer RNAs for suppression of premature termination codons
Engineered transfer RNAs for suppression of premature termination codons Open
Premature termination codons (PTCs) are responsible for 10-15% of all inherited disease. PTC suppression during translation offers a promising approach to treat a variety of genetic disorders, yet small molecules that promote PTC read-thro…
View article: Replacing voltage sensor arginines with citrulline provides mechanistic insight into charge versus shape
Replacing voltage sensor arginines with citrulline provides mechanistic insight into charge versus shape Open
Voltage-dependent activation of voltage-gated cation channels results from the outward movement of arginine-bearing helices within proteinaceous voltage sensors. The voltage-sensing residues in potassium channels have been extensively char…
View article: Rapid evolution of a voltage-gated sodium channel gene in a lineage of electric fish leads to a persistent sodium current
Rapid evolution of a voltage-gated sodium channel gene in a lineage of electric fish leads to a persistent sodium current Open
Most weakly electric fish navigate and communicate by sensing electric signals generated by their muscle-derived electric organs. Adults of one lineage (Apteronotidae), which discharge their electric organs in excess of 1 kHz, instead have…
View article: Orthogonality of Pyrrolysine tRNA in the Xenopus oocyte
Orthogonality of Pyrrolysine tRNA in the Xenopus oocyte Open
Chemical aminoacylation of orthogonal tRNA allows for the genetic encoding of a wide range of synthetic amino acids without the need to evolve specific aminoacyl-tRNA synthetases. This method, when paired with protein expression in the Xen…
View article: Engineered Transfer RNA Suppression of CFTR Nonsense Mutations
Engineered Transfer RNA Suppression of CFTR Nonsense Mutations Open
View article: Contribution of the Hydroxyl Group and Phenol Ring of Tyrosine 780 of the Alpha-Subunit to NA+ Binding by the Na/K Pump
Contribution of the Hydroxyl Group and Phenol Ring of Tyrosine 780 of the Alpha-Subunit to NA+ Binding by the Na/K Pump Open
View article: Site-Specific Encoding of Citrulline, a Neutral Analog of Arginine, to Study the Atomic Basis for Voltage-Dependent Gating In Ion Channels
Site-Specific Encoding of Citrulline, a Neutral Analog of Arginine, to Study the Atomic Basis for Voltage-Dependent Gating In Ion Channels Open
View article: The Role of Backbone Hydrogen Bonds in the Voltage Sensor of K+ Channels
The Role of Backbone Hydrogen Bonds in the Voltage Sensor of K+ Channels Open
View article: Orthogonality of a Synthetic Pyrrolysine tRNA in the Xenopus Oocyte and its use to Encode Unnatural Amino Acids into Ion Channels
Orthogonality of a Synthetic Pyrrolysine tRNA in the Xenopus Oocyte and its use to Encode Unnatural Amino Acids into Ion Channels Open
View article: Insights into Sodium Channel Gating Enabled by Transplantation of an Aryl Sulfonamide Drug Binding Site in Combination with Genetically-Encoded Cross Linking
Insights into Sodium Channel Gating Enabled by Transplantation of an Aryl Sulfonamide Drug Binding Site in Combination with Genetically-Encoded Cross Linking Open
View article: Insulin signaling via the PI3-kinase/Akt pathway regulates airway glucose uptake and barrier function in a CFTR-dependent manner
Insulin signaling via the PI3-kinase/Akt pathway regulates airway glucose uptake and barrier function in a CFTR-dependent manner Open
Cystic fibrosis-related diabetes is the most common comorbidity associated with cystic fibrosis (CF) and correlates with increased rates of lung function decline. Because glucose is a nutrient present in the airways of patients with bacter…
View article: Divergence in Domain IV of an Electric Fish Na V Channel Tunes its Fast Inactivation to Support Rapid Firing Rates by Electro-Motorneurons
Divergence in Domain IV of an Electric Fish Na V Channel Tunes its Fast Inactivation to Support Rapid Firing Rates by Electro-Motorneurons Open
View article: Functional Effects of Substitutions Modifying the Hydroxyl or Phenol Ring of Tyrosine 780 at Ion Binding Site-III of the Na, K-Atpase
Functional Effects of Substitutions Modifying the Hydroxyl or Phenol Ring of Tyrosine 780 at Ion Binding Site-III of the Na, K-Atpase Open
View article: Cellular encoding of Cy dyes for single-molecule imaging
Cellular encoding of Cy dyes for single-molecule imaging Open
A general method is described for the site-specific genetic encoding of cyanine dyes as non-canonical amino acids (Cy-ncAAs) into proteins. The approach relies on an improved technique for nonsense suppression with in vitro misacylated ort…