Daniel W. McVicar
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View article: A simple liquid 3D cell culture paradigm models oxidative mitochondrial metabolism of epithelial breast cancer cells with relevance for lung metastases
A simple liquid 3D cell culture paradigm models oxidative mitochondrial metabolism of epithelial breast cancer cells with relevance for lung metastases Open
Three-dimensional (3D) cell culture systems have emerged as powerful tools to model tumor biology ex vivo. However, the diverse array of 3D culture methods available presents challenges in selecting the most appropriate model for specific …
View article: Elevated tumor NOS2/COX2 promotes immunosuppressive phenotypes associated with poor survival in ER– breast cancer
Elevated tumor NOS2/COX2 promotes immunosuppressive phenotypes associated with poor survival in ER– breast cancer Open
Tumor immunosuppression affects survival and treatment efficacy. Tumor NOS2/COX2 coexpression strongly predicts poor outcome in estrogen receptor-negative (ER-) breast cancer by promoting metastasis, drug resistance, cancer stemness, and i…
View article: Rapid enrichment of progenitor exhausted neoantigen-specific CD8 T cells from peripheral blood
Rapid enrichment of progenitor exhausted neoantigen-specific CD8 T cells from peripheral blood Open
Neoantigen-reactive peripheral blood lymphocytes (NeoPBL) are tumor-specific T cells found at ultra-low frequencies in the blood. Unlike tumor-infiltrating lymphocytes (TIL), NeoPBL exist in a favorable less dysfunctional phenotypic state …
View article: Supplementary Table II from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Table II from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Supplementary Table II summarizes �8+ T cells in designated regions of the tumors.
View article: Supplementary Figure 6 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 6 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Modulation of EpCAM and CD44v6+.
View article: Supplementary Figure 3 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 3 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Immune and tumor quantification
View article: Supplementary Figure 4 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 4 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Supplementary Fig. 4. Supplementary Fig. 4. Spatial UMAP analysis of CD8+/-NOS2+/-COX2+/- phenotypes in Deceased vs Alive patient tumors. Single cell neighborhood profile summary.
View article: Supplementary Figure 1 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 1 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Tumor NOS2 and COX2 expression induced by IFNg and TNFa
View article: Supplementary Figure 4 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 4 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Supplementary Fig. 4. Supplementary Fig. 4. Spatial UMAP analysis of CD8+/-NOS2+/-COX2+/- phenotypes in Deceased vs Alive patient tumors. Single cell neighborhood profile summary.
View article: Supplementary Table II from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Table II from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Supplementary Table II summarizes �8+ T cells in designated regions of the tumors.
View article: Supplementary Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Spatial dot plot of CD8+/-NOS2+/-COX2+/- phenotypes in tumor from deceased patient.
View article: Supplementary Table I from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Table I from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Supplementary Table I summarizes pathological features of tumor immune microenvironment where NOS2+ inflamed regions are significantly higher in tumors from Deceased patients.
View article: Supplementary Figure 1 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 1 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Tumor NOS2 and COX2 expression induced by IFNg and TNFa
View article: Supplementary Figure 2 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 2 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Tumor analysis of NOS2 and COX2.
View article: Supplementary Table I from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Table I from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Supplementary Table I summarizes pathological features of tumor immune microenvironment where NOS2+ inflamed regions are significantly higher in tumors from Deceased patients.
View article: Supplementary Figure 2 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 2 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Tumor analysis of NOS2 and COX2.
View article: Supplementary Figure 6 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 6 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Modulation of EpCAM and CD44v6+.
View article: Supplementary Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Spatial dot plot of CD8+/-NOS2+/-COX2+/- phenotypes in tumor from deceased patient.
View article: Supplementary Figure 3 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 3 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Immune and tumor quantification
View article: HTLV-1 p13 Protein Hijacks Macrophage Polarization and Promotes T-Cell Recruitment
HTLV-1 p13 Protein Hijacks Macrophage Polarization and Promotes T-Cell Recruitment Open
The human T-cell leukemia type-1 (HTLV-1) retrovirus establishes chronic life-long infection in a fraction of infected individuals associated with severe pathological conditions. Although the mechanism driving disease development is not fu…
View article: Targeting Metabolic Programming in the Tumor Microenvironment
Targeting Metabolic Programming in the Tumor Microenvironment Open
In cancer, metabolic adaptations such as fatty acid oxidation are frequently associated with immune suppression, oxidative stress and tumor promotion. Using unbiased metabolomics and quantitative PCR, we found that itaconate, the product o…
View article: Nitric oxide inhibits ten-eleven translocation DNA demethylases to regulate 5mC and 5hmC across the genome
Nitric oxide inhibits ten-eleven translocation DNA demethylases to regulate 5mC and 5hmC across the genome Open
View article: Supplementary Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Spatial dot plot of CD8+/-NOS2+/-COX2+/- phenotypes in tumor from deceased patient.
View article: Supplementary Table II from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Table II from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Supplementary Table II summarizes �8+ T cells in designated regions of the tumors.
View article: Figure 6 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Figure 6 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
S-UMAP and regional annotations identify cellular neighborhoods of interest with respect to the tumor CSC markers EpCAM and CD44v6. A, Unsupervised analysis of S-UMAP highlighting distinct EpCAM and CD44v6 expression in specific mag…
View article: Figure 3 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Figure 3 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
S-UMAP analysis for tumor NOS2/COX2 and CD8 expression. Density heat maps show spatially distinct tumor NOS2, COX2, and CD8 clustering in tumors from (A) deceased and (B) alive patients. Spatial distribution plots based upon …
View article: Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Figure 5 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Spatial landscape of CD44v6 and EpCAM expression relative to tumor NOS2/COX2 expression and survival. A, Enriched regions showing spatially distinct CD44v6 and EpCAM expression. B, Quantification of CD44v6 and EpCAM as well a…
View article: Supplementary Figure 1 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 1 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Tumor NOS2 and COX2 expression induced by IFNg and TNFa
View article: Supplementary Figure 2 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Supplementary Figure 2 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Tumor analysis of NOS2 and COX2.
View article: Figure 4 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer
Figure 4 from Tumor NOS2 and COX2 Spatial Juxtaposition with CD8<sup>+</sup> T Cells Promote Metastatic and Cancer Stem Cell Niches that Lead to Poor Outcome in ER<sup>−</sup> Breast Cancer Open
Defined CD8 NOS2 and COX2 spatial landscape. Five basic regions showing (A) margin or stroma-restricted lymphoid aggregates (orange circles) in which a gap of 50 μm (double orange arrow) between CD3+ T-cell aggregates and…