Daniel Žucha
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View article: Integrated multi-omics profiling uncovers miRNA-guided regulatory networks after spinal cord injury in rats
Integrated multi-omics profiling uncovers miRNA-guided regulatory networks after spinal cord injury in rats Open
Spinal cord injury (SCI) is a debilitating condition with no effective treatment. The injury triggers a complex cascade of molecular and cellular events that drive both damage and repair processes. To explore these mechanisms, we performed…
View article: Integrated omics reveals disease-associated radial glia-like cells with epigenetically dysregulated interferon response in multiple sclerosis
Integrated omics reveals disease-associated radial glia-like cells with epigenetically dysregulated interferon response in multiple sclerosis Open
Progressive multiple sclerosis (PMS) involves a persistent, maladaptive inflammatory process with numerous cellular drivers. We generated induced neural stem cells (iNSCs) from patient fibroblasts through a direct reprogramming protocol th…
View article: Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia
Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia Open
The role of nonneuronal cells in the resolution of cerebral ischemia remains to be fully understood. To decode key molecular and cellular processes that occur after ischemia, we performed spatial and single-cell transcriptomic profiling of…
View article: Characterization of regeneration initiating cells during Xenopus laevis tail regeneration
Characterization of regeneration initiating cells during Xenopus laevis tail regeneration Open
View article: NEUROD1 reinforces endocrine cell fate acquisition in pancreatic development
NEUROD1 reinforces endocrine cell fate acquisition in pancreatic development Open
View article: Characterization of regeneration initiating cells during<i>Xenopus laevis</i>tail regeneration
Characterization of regeneration initiating cells during<i>Xenopus laevis</i>tail regeneration Open
Embryos are regeneration and wound healing masters. They not only rapidly close their wounds, remodel injured tissue without a scar, but also regenerate body parts. Many animal models with variable regenerative capabilities have already be…
View article: Complement C3a treatment accelerates recovery after stroke via modulation of astrocyte reactivity and cortical connectivity
Complement C3a treatment accelerates recovery after stroke via modulation of astrocyte reactivity and cortical connectivity Open
Despite advances in acute care, ischemic stroke remains a major cause of long-term disability. Approaches targeting both neuronal and glial responses are needed to enhance recovery and improve long-term outcome. The complement C3a receptor…
View article: Spatiotemporal transcriptomic map of ischemic brain injury
Spatiotemporal transcriptomic map of ischemic brain injury Open
The role of non-neuronal cells in the resolution of cerebral ischemia remains to be fully understood. To decode key cellular processes that occur after ischemia, we performed spatial and single-cell transcriptomic profiling of mouse brain …
View article: ISL1 controls pancreatic alpha cell fate and beta cell maturation
ISL1 controls pancreatic alpha cell fate and beta cell maturation Open
Background Glucose homeostasis is dependent on functional pancreatic α and ß cells. The mechanisms underlying the generation and maturation of these endocrine cells remain unclear. Results We unravel the molecular mode of action of ISL1 in…
View article: Recent advances in deciphering oligodendrocyte heterogeneity with single-cell transcriptomics
Recent advances in deciphering oligodendrocyte heterogeneity with single-cell transcriptomics Open
Oligodendrocytes (OL) have been for decades considered a passive, homogenous population of cells that provide support to neurons, and show a limited response to pathological stimuli. This view has been dramatically changed by the introduct…
View article: Compromised Astrocyte Swelling/Volume Regulation in the Hippocampus of the Triple Transgenic Mouse Model of Alzheimer’s Disease
Compromised Astrocyte Swelling/Volume Regulation in the Hippocampus of the Triple Transgenic Mouse Model of Alzheimer’s Disease Open
In this study, we aimed to disclose the impact of amyloid-β toxicity and tau pathology on astrocyte swelling, their volume recovery and extracellular space (ECS) diffusion parameters, namely volume fraction (α) and tortuosity (λ), in a tri…
View article: Tutorial: Guidelines for Single-Cell RT-qPCR
Tutorial: Guidelines for Single-Cell RT-qPCR Open
Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility i…
View article: Transient astrocyte‐like <scp>NG2</scp> glia subpopulation emerges solely following permanent brain ischemia
Transient astrocyte‐like <span>NG2</span> glia subpopulation emerges solely following permanent brain ischemia Open
A bstract NG2 glia display wide proliferation and differentiation potential under physiological and pathological conditions. Here, we examined these two features following different types of brain disorders such as focal cerebral ischemia …
View article: Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain
Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain Open
View article: Performance Comparison of Reverse Transcriptases for Single-Cell Studies
Performance Comparison of Reverse Transcriptases for Single-Cell Studies Open
BACKGROUND Recent advances allowing quantification of RNA from single cells are revolutionizing biology and medicine. Currently, almost all single-cell transcriptomic protocols rely on reverse transcription (RT). However, RT is recognized …
View article: Decoding the transcriptional response to ischemic stroke in young and aged mouse brain
Decoding the transcriptional response to ischemic stroke in young and aged mouse brain Open
Ischemic stroke is one of the leading causes of mortality and major healthcare and economic burden. It is a well-recognized disease of aging, yet it is unclear how the age-dependent vulnerability occurs and what are the underlying mechanis…
View article: Performance comparison of reverse transcriptases for single-cell studies
Performance comparison of reverse transcriptases for single-cell studies Open
Background Recent technical advances allowing quantification of RNA from single cells are revolutionizing biology and medicine. Currently, almost all single-cell transcriptomic protocols rely on conversion of RNA to cDNA by reverse transcr…