Danielle Middleton
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View article: Culture‐Independent Species‐Level Taxonomic and Functional Characterisation of <i>Bacteroides</i> , the Core Bacterial Genus Within Reptile Guts
Culture‐Independent Species‐Level Taxonomic and Functional Characterisation of <i>Bacteroides</i> , the Core Bacterial Genus Within Reptile Guts Open
The genus Bacteroides is a widespread and abundant bacterial taxon associated with gut microbiotas. Species within Bacteroides fill many niches, including as mutualists, commensals and pathogens for their hosts. Within many reptiles, Bacte…
View article: Benchmarking 16S rRNA Gene‐Based Approaches to Bacterial Taxonomy Assignment Based on Amplicon Sequencing With Illumina and Oxford Nanopore
Benchmarking 16S rRNA Gene‐Based Approaches to Bacterial Taxonomy Assignment Based on Amplicon Sequencing With Illumina and Oxford Nanopore Open
Research investigating the microbial community of an ecosystem or animal can involve a range of methodologies, including sequencing technology, bioinformatic software and taxonomy database. Researchers may utilise short‐read sequencing on …
View article: Limited gut bacterial response of tuatara (<i>Sphenodon punctatus</i>) to dietary manipulation and captivity
Limited gut bacterial response of tuatara (<i>Sphenodon punctatus</i>) to dietary manipulation and captivity Open
The bacteria of a host’s digestive tract play crucial roles in digestion and pathogen resistance. Hosts living in captivity often have more human interaction and antibiotic use, in addition to differences in diet and environment, compared …
View article: Common commercially available parasiticides do not cause fatal changes in the microbiome of the dung beetle <i>Onthophagus binodis</i> –a pilot study
Common commercially available parasiticides do not cause fatal changes in the microbiome of the dung beetle <i>Onthophagus binodis</i> –a pilot study Open
Dung beetles perform vital ecosystem functions, but their survival is threatened by parasiticide use in veterinary practices. Currently, it's unclear if parasiticides directly harm dung beetles or cause damage via secondary effects on cruc…
View article: Gut microbiome of the sole surviving member of reptile order Rhynchocephalia reveals biogeographic variation, influence of host body condition and a substantial core microbiota in tuatara across New Zealand
Gut microbiome of the sole surviving member of reptile order Rhynchocephalia reveals biogeographic variation, influence of host body condition and a substantial core microbiota in tuatara across New Zealand Open
Tuatara are the sole extant species in the reptile order Rhynchocephalia. They are ecologically and evolutionarily unique, having been isolated geographically for ~84 million years and evolutionarily from their closest living relatives for…
View article: Additional file 2 of Gut microbiota predicts body fat change following a low-energy diet: a PREVIEW intervention study
Additional file 2 of Gut microbiota predicts body fat change following a low-energy diet: a PREVIEW intervention study Open
Additional file 2. Two supporting tables (Table S1-S2) of lists of input predictors for body fat change in random forest regression ranked by feature importance. A table caption for each table is given within the file.
View article: The airway microbiota in children newly diagnosed with bronchiectasis largely retains its diversity
The airway microbiota in children newly diagnosed with bronchiectasis largely retains its diversity Open
Young children diagnosed with bronchiectasis have a lower airway microbiota that is highly diverse and very similar to healthy controls. Haemophilus, Moraxella, Neisseria and Streptococcus are the most abundant operational taxonomic units …
View article: An Examination of Nonprofit Lobbying Expenditures
An Examination of Nonprofit Lobbying Expenditures Open
Nonprofit organizations are characterized by both their programmatic and advocacy activity. Nonprofits are called on not only to provide essential services for many citizens, but also to advocate on the behalf of the issues impacting the p…
View article: Integrity of the Human Faecal Microbiota following Long-Term Sample Storage
Integrity of the Human Faecal Microbiota following Long-Term Sample Storage Open
In studies of the human microbiome, faecal samples are frequently used as a non-invasive proxy for the study of the intestinal microbiota. To obtain reliable insights, the need for bacterial DNA of high quality and integrity following appr…