Christoph Grunau
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View article: Not just mutations: Inbreeding depression persists without genetic variation
Not just mutations: Inbreeding depression persists without genetic variation Open
Inbreeding depression (ID), the decline in fitness upon inbreeding, is thought to result from a decrease in genetic heterozygosity enhancing phenotypic effects of recessive deleterious mutations. However, emerging evidence suggests that mu…
View article: Benchmarking of Chromatin Immunoprecipitation (ChIP-Seq) methods in the Pacific oyster Magallana gigas
Benchmarking of Chromatin Immunoprecipitation (ChIP-Seq) methods in the Pacific oyster Magallana gigas Open
View article: Benchmarking of Chromatin Immunoprecipitation (ChIP-Seq) methods in the Pacific oyster<i>Magallana gigas</i>
Benchmarking of Chromatin Immunoprecipitation (ChIP-Seq) methods in the Pacific oyster<i>Magallana gigas</i> Open
Chromatin Immunoprecipitation sequencing (ChIP-seq) is the method of choice to generate chromatin landscapes across genomes. The scarcity of literature on ChIPseq and absence of a canonical “gold standard” method in mollusks and especially…
View article: Heterochromatin protein 1 (HP1) of<i>Schistosoma mansoni</i>: non-canonical chromatin landscape and fitness effects
Heterochromatin protein 1 (HP1) of<i>Schistosoma mansoni</i>: non-canonical chromatin landscape and fitness effects Open
Heterochromatin protein 1 (HP1) is widespread in several organisms playing a role in control of gene expression by heterochromatin formation and maintenance of silent chromatin. Schistosoma mansoni is a human parasite that is responsible f…
View article: Chromatin structure around long non-coding RNA (lncRNA) genes in<i>Schistosoma mansoni</i>gonads
Chromatin structure around long non-coding RNA (lncRNA) genes in<i>Schistosoma mansoni</i>gonads Open
In this study, we employed a total of eight distinct modifications of histone proteins (H3K23ac, H3K27me3, H3K36me3, H3K4me3, H3K9ac, H3K9me3, H4K12ac, and H4K20me1) to discern the various chromatin colors encompassing lncRNA genes in both…
View article: Supporting information for "Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni"
Supporting information for "Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni" Open
Files “CG_Ro_1_High Sensitivity DNA Assay_DE13805677_2019-06-20_09-10-48.pdf” and “CG_Ro_2_High Sensitivity DNA Assay_DE13805677_2019-06-20_10-12-12.pdf” uncropped files used in figure 2 File “gel qpcr test input sds_01.tif” : uncropped fi…
View article: Heterochromatin protein 1 (HP1) of Schistosoma mansoni : non-canonical chromatin landscape and oviposition effects
Heterochromatin protein 1 (HP1) of Schistosoma mansoni : non-canonical chromatin landscape and oviposition effects Open
International audience
View article: Mécanismes épigénétiques des interactions hôte-agent pathogène
Mécanismes épigénétiques des interactions hôte-agent pathogène Open
International audience
View article: Enhanced Efficiency of Rna-guided Cas12a Versus Cas9 Transgene Knock-in and Activity at a Schistosoma Mansoni Genome Safe Harbor
Enhanced Efficiency of Rna-guided Cas12a Versus Cas9 Transgene Knock-in and Activity at a Schistosoma Mansoni Genome Safe Harbor Open
Recently, we reported programmed Cas9 mediated insertion of a reporter gene into a gene safe harbor site, GSH1, of Schistosoma mansoni via homology-directed repair (HDR) using overlapping guide RNAs. Here, we report efficient and precise C…
View article: ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A<i>SCHISTOSOMA MANSONI</i>GENOME SAFE HARBOR
ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A<i>SCHISTOSOMA MANSONI</i>GENOME SAFE HARBOR Open
Recently, we reported programmed Cas9 mediated insertion of a reporter gene into a gene safe harbor site, GSH1, of Schistosoma mansoni via homology-directed repair (HDR) using overlapping guide RNAs. Here, we report efficient and precise C…
View article: Epigenetic variations are more substantial than genetic variations in rapid adaptation of oyster to Pacific oyster mortality syndrome
Epigenetic variations are more substantial than genetic variations in rapid adaptation of oyster to Pacific oyster mortality syndrome Open
Disease emergence is accelerating with global changes. Understanding by which mechanisms host populations can rapidly adapt will be crucial for management practices. Pacific oyster mortality syndrome (POMS) imposes a substantial and recurr…
View article: Epigenetic variation causes heritable variation in complex traits in the mollusk<i>Biomphalaria glabrata</i>, vector of the human parasite<i>Schistosoma mansoni</i>
Epigenetic variation causes heritable variation in complex traits in the mollusk<i>Biomphalaria glabrata</i>, vector of the human parasite<i>Schistosoma mansoni</i> Open
DNA methylation variation may play a role in phenotypic variation as it can be directly affected by the environment and be inherited. DNA methylation variations were introduced into the parasite vector snail Biomphalaria glabrata with low …
View article: Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni
Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni Open
View article: An epigenetic molluscicide
An epigenetic molluscicide Open
Biomphalaria glabrata is a fresh-water mollusk that serves as obligatory intermediate host to Schistosoma mansoni , agent of the neglected tropical disease schistosomiasis that affects roughly 250 Mio people. One of the ways to control the…
View article: Epigenetic then genetic variations underpin rapid adaptation of oyster populations (<i>Crassostrea gigas</i>) to Pacific Oyster Mortality Syndrome (POMS)
Epigenetic then genetic variations underpin rapid adaptation of oyster populations (<i>Crassostrea gigas</i>) to Pacific Oyster Mortality Syndrome (POMS) Open
Disease emergence is accelerating in response to human activity-induced global changes. Understanding the mechanisms by which host populations can rapidly adapt to this threat will be crucial for developing future management practices. Pac…
View article: Datasets for "Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni"
Datasets for "Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni" Open
To identify sites that could serve as potential genomic safe harbours (GSHs) for transgene integration, we conducted a genome-wide bioinformatic search based on established, widely accepted criteria, along with newly introduced criteria (b…
View article: Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures
Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures Open
Extended data This project contains the following extended data: - Fig S1. Dose response titrations of compounds 15, 16, 33, 35 and 36 against schistosomula. - Fig S2. Compound 33 treatment induces the rel…
View article: Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures
Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures Open
Extended data This project contains the following extended data: - Fig S1. Dose response titrations of compounds 15, 16, 33, 35 and 36 against schistosomula. - Fig S2. Compound 33 treatment induces the rel…
View article: Datasets for "Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni"
Datasets for "Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni" Open
To identify sites that could serve as potential genomic safe harbours (GSHs) for transgene integration, we conducted a genome-wide bioinformatic search based on established, widely accepted criteria, along with newly introduced criteria (b…
View article: Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures
Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures Open
Extended data This project contains the following extended data: - Fig S1. Dose response titrations of compounds 15, 16, 33, 35 and 36 against schistosomula. - Fig S2. Compound 33 treatment induces the rel…
View article: Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures
Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures Open
Extended data This project contains the following extended data: - Fig S1. Dose response titrations of compounds 15, 16, 33, 35 and 36 against schistosomula. - Fig S2. Compound 33 treatment induces the rel…
View article: Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures
Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary Figures Open
Extended data This project contains the following extended data: - Fig S1. Dose response titrations of compounds 15, 16, 33, 35 and 36 against schistosomula. - Fig S2. Compound 33 treatment induces the rel…
View article: Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary tables and movies
Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary tables and movies Open
Extended data This project contains the following extended data: Table S1. Chemical structures of the 39 compounds included in this study. Table S2. Z´ values for both phenotype and motility of the Robowo…
View article: Schistosoma mansoni ATAC-seq results for IGV (female and male worms with and without LSD1 inhibitor)
Schistosoma mansoni ATAC-seq results for IGV (female and male worms with and without LSD1 inhibitor) Open
In this study, the anti-schistosomal activity of 39 Homo sapiens Lysine Specific Demethylase 1 (HsLSD1) inhibitors was investigated on parasitic life cycle stages associated with both definitive and intermediate host infection. Am…
View article: Schistosoma mansoni ATAC-seq results for IGV (female and male worms with and without LSD1 inhibitor)
Schistosoma mansoni ATAC-seq results for IGV (female and male worms with and without LSD1 inhibitor) Open
In this study, the anti-schistosomal activity of 39 Homo sapiens Lysine Specific Demethylase 1 (HsLSD1) inhibitors was investigated on parasitic life cycle stages associated with both definitive and intermediate host infection. Am…
View article: Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke,<i>Schistosoma mansoni</i>
Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke,<i>Schistosoma mansoni</i> Open
Summary The identification and characterization of genomic safe harbor sites (GSH) aims to facilitate consistent transgene activity without disruption to the host cell genome. We combined genome annotation and chromatin structure analysis …
View article: Déterminants et poids relatif de la variation génétique et épigénétique dans la résistance de l'huître Crassostrea gigas au pacific oyster mortality Syndrome
Déterminants et poids relatif de la variation génétique et épigénétique dans la résistance de l'huître Crassostrea gigas au pacific oyster mortality Syndrome Open
Together with the increase of human population, there is a mathematical increase for food supply. Since 2008, mass mortality events of Pacific oysters (Crassostrea gigas) juveniles caused by the Pacific Oyster Mortality Syndrome (POMS) hav…
View article: Supporting information for "Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni"
Supporting information for "Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni" Open
Files “CG_Ro_1_High Sensitivity DNA Assay_DE13805677_2019-06-20_09-10-48.pdf” and “CG_Ro_2_High Sensitivity DNA Assay_DE13805677_2019-06-20_10-12-12.pdf” uncropped files used in figure 2 File “gel qpcr test input sds_01.tif” : uncropped f…
View article: Supporting information for "Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni"
Supporting information for "Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni" Open
Files “CG_Ro_1_High Sensitivity DNA Assay_DE13805677_2019-06-20_09-10-48.pdf” and “CG_Ro_2_High Sensitivity DNA Assay_DE13805677_2019-06-20_10-12-12.pdf” cropped in figure 2 File “gel qpcr test input sds_01.tif” cropped in figure 8. Produc…
View article: Hit-and-Run Epigenetic Editing for Vectors of Snail-Borne Parasitic Diseases
Hit-and-Run Epigenetic Editing for Vectors of Snail-Borne Parasitic Diseases Open
Snail-borne parasitic diseases represent an important challenge to human and animal health. Control strategies that target the intermediate snail host has proved very effective. Epigenetic mechanisms are involved in developmental processes…