Darren Greetham
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View article: Thermo-mechanical pre-treatments modulate the structure, protein and starch digestibility, and thermal and pasting properties of sorghum flour
Thermo-mechanical pre-treatments modulate the structure, protein and starch digestibility, and thermal and pasting properties of sorghum flour Open
This study examined the effects of five thermal-mechanical pre-treatments, including popping, roasting, parboiling, pressure cooking, and extrusion, on the structure, digestibility, and thermal and pasting properties of sorghum flour. The …
View article: Clostridioides difficile canonical L,D-transpeptidases catalyze a novel type of peptidoglycan cross-links and are not required for beta-lactam resistance
Clostridioides difficile canonical L,D-transpeptidases catalyze a novel type of peptidoglycan cross-links and are not required for beta-lactam resistance Open
Clostridioides difficile is the leading cause of antibiotic-associated diarrhea worldwide with significant morbidity and mortality. This organism is naturally resistant to several beta-lactam antibiotics that inhibit the polymerization of …
View article: Unusual 1-3 peptidoglycan cross-links in<i>Acetobacteraceae</i>are made by L,D-transpeptidases with a catalytic domain distantly related to YkuD domains
Unusual 1-3 peptidoglycan cross-links in<i>Acetobacteraceae</i>are made by L,D-transpeptidases with a catalytic domain distantly related to YkuD domains Open
Peptidoglycan is an essential component of the bacterial cell envelope that contains glycan chains substituted by short peptide stems. Peptide stems are polymerized by D,D-transpeptidases, which make bonds between the amino acid in positio…
View article: The characterisation of Wickerhamomyces anomalus M15, a highly tolerant yeast for bioethanol production using seaweed derived medium
The characterisation of Wickerhamomyces anomalus M15, a highly tolerant yeast for bioethanol production using seaweed derived medium Open
Advanced generation biofuels have potential for replacing fossil fuels as society moves forward into a net-zero carbon future. Marine biomass is a promising source of fermentable sugars for fermentative bioethanol production; however the m…
View article: Exploring the Bioethanol Production Potential of Miscanthus Cultivars
Exploring the Bioethanol Production Potential of Miscanthus Cultivars Open
Miscanthus is a fast-growing perennial grass that attracts significant attention for its potential application as a feedstock for bioethanol production. This report explores the difference in the lignocellulosic composition of various Misc…
View article: Impact of mixed feedstock of wheat straw, willow and <i>Miscanthus</i> on enzymatic hydrolysis and inhibitor production after microwave hydrothermal pre-treatment in Europe
Impact of mixed feedstock of wheat straw, willow and <i>Miscanthus</i> on enzymatic hydrolysis and inhibitor production after microwave hydrothermal pre-treatment in Europe Open
Lignocellulosic feedstocks for biorefinery are likely to be seasonal and the supply of feedstock to cellulosic biorefineries remains a challenge. One way to overcome this is by utilizing a mixed feedstock which facilitates the maintenance …
View article: Viability of Municipal Solid Waste as a source for Bioenergy products production
Viability of Municipal Solid Waste as a source for Bioenergy products production Open
Energy is an important requirement for population growth, technological progress and urbanisation. Worldwide energy demand has been projected to increase 5-fold by 2100. Fulfilment of these energy requirements cannot be solely reliant on f…
View article: A Role for COX20 in Tolerance to Oxidative Stress and Programmed Cell Death in Saccharomyces cerevisiae
A Role for COX20 in Tolerance to Oxidative Stress and Programmed Cell Death in Saccharomyces cerevisiae Open
Industrial production of bioethanol from lignocellulosic materials (LCM′s) is reliant on a microorganism being tolerant to the stresses inherent to fermentation. Previous work has highlighted the importance of a cytochrome oxidase chaperon…
View article: The Development of a Sorghum Bran-Based Biorefining Process to Convert Sorghum Bran into Value Added Products
The Development of a Sorghum Bran-Based Biorefining Process to Convert Sorghum Bran into Value Added Products Open
Sorghum bran, a starch rich food processing waste, was investigated for the production of glucoamylase in submerged fungal fermentation using Aspergillus awamori. The fermentation parameters, such as cultivation time, substrate concentrati…
View article: Exploring the tolerance of marine yeast to inhibitory compounds for improving bioethanol production
Exploring the tolerance of marine yeast to inhibitory compounds for improving bioethanol production Open
Inhibitor tolerance is one of the key challenges in lignocellulosic bioethanol production.
View article: Optimized Conditions for Bioleaching using Yeasts from Municipal Solid Wastes to produce Safe Compost or Fertiliser
Optimized Conditions for Bioleaching using Yeasts from Municipal Solid Wastes to produce Safe Compost or Fertiliser Open
Bioleaching can be considering a simple and effective technology for removing metals from waste which is rich in heavy metals. Bioleaching of these metals by microorganisms offers an economical and eco-friendly approach. In this study Sacc…
View article: Identification of Ethanologenic Yeast Strains from Wild Habitats
Identification of Ethanologenic Yeast Strains from Wild Habitats Open
Bioethanol generated from household, agricultural or forestry waste is currently being pursued as the second generation biofuel with wheat straw being highlighted as a promising lignocellulosic residue feedstock in the UK. Natural fermenta…
View article: Optimization of Parameters for Sugar Releasing from Municipal Solid Waste (MSW)
Optimization of Parameters for Sugar Releasing from Municipal Solid Waste (MSW) Open
Treatment of Municipal Solid Waste (MSW) becomes an increasing problem worldwide; however, MSW contains high lignocellulosic content that can be further processed for the generation of electricity, transportation fuel and other commerciall…
View article: Effect of Operating Conditions on Molasses Fermentation for Bioethanol Production
Effect of Operating Conditions on Molasses Fermentation for Bioethanol Production Open
The aim of the presented study was to evaluate the potential of molasses as bioethanol feedstock by studying the effect of different operating conditions on fermentation yield including initial sugar concentration (25-150 g/L), pH (4.5-9.5…
View article: The Application of Fungi for Bioleaching of Municipal Solid Wastes for the Production of Environmental Acceptable Compost Production
The Application of Fungi for Bioleaching of Municipal Solid Wastes for the Production of Environmental Acceptable Compost Production Open
Analysis of the chemical components of Municipal solid waste (MSW) indicated that the presence of high concentrations of toxic heavy metals. The aim of this study was to select the best leaching condition, in order to generate a compost pr…
View article: A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology
A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology Open
Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. The…
View article: Evaluation of different lignocellulosic biomass pretreatments by phenotypic microarray-based metabolic analysis of fermenting yeast
Evaluation of different lignocellulosic biomass pretreatments by phenotypic microarray-based metabolic analysis of fermenting yeast Open
Advanced generation biofuel production from lignocellulosic material (LCM) was investigated. A range of different thermo-chemical pre-treatments were evaluated with different LCM. The pre-treatments included; alkaline (5% NaOH at 50°C), ac…
View article: Sodium ion interaction with psyllium husk (Plantago sp.)
Sodium ion interaction with psyllium husk (Plantago sp.) Open
The nature of and factors effecting sodium interaction with psyllium were investigated in vitro. The feasibility of use of psyllium husk to reduce the bioavailable fraction of ingested sodium is demonstrated.
View article: Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)
Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF) Open
This paper explores the possibility of using an industrially processed municipal solid waste (MSW) for cellulase enzyme production via solid state fermentation (SSF) by Trichoderma reesei and Aspergillus niger. Both fungi grew well on the …
View article: Expression of Mitochondrial Cytochrome C Oxidase Chaperone Gene (COX20) Improves Tolerance to Weak Acid and Oxidative Stress during Yeast Fermentation
Expression of Mitochondrial Cytochrome C Oxidase Chaperone Gene (COX20) Improves Tolerance to Weak Acid and Oxidative Stress during Yeast Fermentation Open
This is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.
View article: Screening of Non- Saccharomyces cerevisiae Strains for Tolerance to Formic Acid in Bioethanol Fermentation
Screening of Non- Saccharomyces cerevisiae Strains for Tolerance to Formic Acid in Bioethanol Fermentation Open
Formic acid is one of the major inhibitory compounds present in hydrolysates derived from lignocellulosic materials, the presence of which can significantly hamper the efficiency of converting available sugars into bioethanol. This study i…
View article: Acetylcholine Protects against Candida albicans Infection by Inhibiting Biofilm Formation and Promoting Hemocyte Function in a Galleria mellonella Infection Model
Acetylcholine Protects against Candida albicans Infection by Inhibiting Biofilm Formation and Promoting Hemocyte Function in a Galleria mellonella Infection Model Open
Both neuronal acetylcholine and nonneuronal acetylcholine have been demonstrated to modulate inflammatory responses. Studies investigating the role of acetylcholine in the pathogenesis of bacterial infections have revealed contradictory fi…