David Alan Brown
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View article: S6P mutation in Delta and Omicron variant spike protein significantly enhances the efficacy of mRNA COVID-19 vaccines
S6P mutation in Delta and Omicron variant spike protein significantly enhances the efficacy of mRNA COVID-19 vaccines Open
Background The unrelenting emergence of SARS-CoV-2 variants has significantly challenged the efficacy of existing COVID-19 vaccines. Enhancing the stability and immunogenicity of the spike protein is critical for improving vaccine performa…
View article: Estimating highest capacity propulsion performance using backward-directed force during walking evaluation for individuals with acquired brain injury
Estimating highest capacity propulsion performance using backward-directed force during walking evaluation for individuals with acquired brain injury Open
There are over 5.3 million Americans who face acquired brain injury (ABI)-related disability as well as almost 800,000 who suffer from stroke each year. To improve mobility and quality of life, rehabilitation professionals often focus on w…
View article: 31. Modulation Of Mammalian Digit Regeneration With Viral Gene Therapy
31. Modulation Of Mammalian Digit Regeneration With Viral Gene Therapy Open
Purpose: A primary focus of regenerative medicine is to understand the cellular mechanisms governing tissue regeneration across various species. Mammals generally possess a restricted regenerative capacity in comparison to species like zeb…
View article: Single-cell RNA sequencing of 76,535 capillary PBMCs from 3 donors across 28 samples, freshly isolated or on ice for 24h with manual 4-layer annotation hierarchy
Single-cell RNA sequencing of 76,535 capillary PBMCs from 3 donors across 28 samples, freshly isolated or on ice for 24h with manual 4-layer annotation hierarchy Open
Sample Procurement Samples were collected directly from participants as part of ImYoo's "Single-cell immune profiling from self-collected capillary blood" study, approved by Advarra IRB (Protocol #Pro00057361). Sample Processing Whole capi…
View article: Single-cell RNA sequencing of 76,535 capillary PBMCs from 3 donors across 28 samples, freshly isolated or on ice for 24h with manual 4-layer annotation hierarchy
Single-cell RNA sequencing of 76,535 capillary PBMCs from 3 donors across 28 samples, freshly isolated or on ice for 24h with manual 4-layer annotation hierarchy Open
Sample Procurement Samples were collected directly from participants as part of ImYoo's "Single-cell immune profiling from self-collected capillary blood" study, approved by Advarra IRB (Protocol #Pro00057361). Sample Processing Whole capi…
View article: Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors
Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors Open
The blood-brain barrier (BBB) presents a major challenge for delivering large molecules to study and treat the central nervous system. This is due in part to the scarcity of targets known to mediate BBB crossing. To identify novel targets,…
View article: A Consensus Approach for Targeted Muscle Reinnervation in Amputees
A Consensus Approach for Targeted Muscle Reinnervation in Amputees Open
Amputations have been performed with few modifications since the dawn of surgery. Blood vessels are ligated, bones are shortened, and nerves are cut. In a percentage of people, this can result in severe neuropathic, residual limb, and phan…
View article: Primate-conserved Carbonic Anhydrase IV and murine-restricted Ly6c1 are new targets for crossing the blood-brain barrier
Primate-conserved Carbonic Anhydrase IV and murine-restricted Ly6c1 are new targets for crossing the blood-brain barrier Open
The blood-brain barrier (BBB) presents a major challenge to delivering large molecules to study and treat the central nervous system (CNS). This is due in part to the scarcity of effective targets for BBB crossing, the identification of wh…
View article: P3. Meeting Student Goals in Virtual Sub-Internships: A Multi-Institutional Experience
P3. Meeting Student Goals in Virtual Sub-Internships: A Multi-Institutional Experience Open
Background: The Coalition for Physician Accountability recommended halting all in-person away rotations for the 2020-2021 residency application cycle. In response, many institutions developed virtual alternatives to in-person rotations. In…
View article: Deep Parallel Characterization of AAV Tropism and AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing
Deep Parallel Characterization of AAV Tropism and AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing Open
Engineered variants of recombinant adeno-associated viruses (rAAVs) are being developed rapidly to meet the need for gene-therapy delivery vehicles with particular cell-type and tissue tropisms. While high-throughput AAV engineering and se…
View article: Single-Cell RNA Sequencing Reveals Cellular and Transcriptional Changes Associated With M1 Macrophage Polarization in Hidradenitis Suppurativa
Single-Cell RNA Sequencing Reveals Cellular and Transcriptional Changes Associated With M1 Macrophage Polarization in Hidradenitis Suppurativa Open
Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease characterized by recurrent abscesses, nodules, and sinus tracts in areas of high hair follicle and sweat gland density. These sinus tracts can present with purulent drain…
View article: Deep parallel characterization of AAV tropism and AAV-mediated transcriptional changes via single-cell RNA sequencing
Deep parallel characterization of AAV tropism and AAV-mediated transcriptional changes via single-cell RNA sequencing Open
Engineered variants of recombinant adeno-associated viruses (rAAVs) are being developed rapidly to meet the need for gene-therapy delivery vehicles with particular cell-type and tissue tropisms. While high-throughput AAV engineering and se…
View article: Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood
Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood Open
An individual’s immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a …
View article: The skin transcriptome in hidradenitis suppurativa uncovers an antimicrobial and sweat gland gene signature which has distinct overlap with wounded skin
The skin transcriptome in hidradenitis suppurativa uncovers an antimicrobial and sweat gland gene signature which has distinct overlap with wounded skin Open
Hidradenitis suppurativa (HS) is a debilitating chronic inflammatory skin disease resulting in non-healing wounds affecting body areas of high hair follicle and sweat gland density. The pathogenesis of HS is not well understood but appears…
View article: Computational Analysis of the Mature Unilateral Cleft Lip Nasal Deformity on Nasal Patency
Computational Analysis of the Mature Unilateral Cleft Lip Nasal Deformity on Nasal Patency Open
Background: Nasal airway obstruction (NAO) due to nasal anatomic deformities is known to be more common among cleft patients than the general population, yet information is lacking regarding severity and variability of cleft-associated nas…
View article: Automatic Detection and Tracking of Plumes from 67P/Churyumov–Gerasimenko in Rosetta/OSIRIS Image Sequences
Automatic Detection and Tracking of Plumes from 67P/Churyumov–Gerasimenko in Rosetta/OSIRIS Image Sequences Open
Solar system bodies such as comets and asteroids are known to eject material from their surface in the form of jets and plumes. Observations of these transient outbursts can offer insight into the inner workings and makeup of their origina…
View article: Deciphering the Language of Etruscan Dress: a Hierarchical Clustering Analysis Re-Tailoring Tarquinian Tomb Painting
Deciphering the Language of Etruscan Dress: a Hierarchical Clustering Analysis Re-Tailoring Tarquinian Tomb Painting Open
Tarquinian tomb paintings are of an unrivalled artistic and cultural importance. The primary aim of this thesis is to identify and investigate their pictorial forms, language, and structures of painted dress, so as to re-tailor understandi…
View article: Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation
Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation Open
Liver synthetic and metabolic function can only be optimised by the growth of cells within a supportive liver matrix. This can be achieved by the utilisation of decellularised human liver tissue. Here we demonstrate complete decellularizat…