David B. Lewis
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View article: Patients with Genetic Immune Diseases in the Hematologist’s Waiting Room
Patients with Genetic Immune Diseases in the Hematologist’s Waiting Room Open
Uncomplicated autoimmune cytopenias in children can present emergently but usually respond to first- or second-line treatment (steroids, immunoglobulin replacement therapy, and +/- rituximab) without long-term sequelae. In contrast, refrac…
View article: Evaluating the Role of Genetic Testing for Inborn Errors of Immunity in Pediatric Patients with Very Early-Onset and Early-Onset Inflammatory Bowel Disease
Evaluating the Role of Genetic Testing for Inborn Errors of Immunity in Pediatric Patients with Very Early-Onset and Early-Onset Inflammatory Bowel Disease Open
Introduction The incidence of inflammatory bowel disease (IBD) has significantly increased in developing countries over the last decade with a rising prevalence among the pediatric population. Very early-onset IBD (VEOIBD), defined as IBD …
View article: P1091 Trends in length of stay and steroid use in patients admitted with Acute Severe Ulcerative Colitis: Impact of intensified or accelerated infliximab dosing
P1091 Trends in length of stay and steroid use in patients admitted with Acute Severe Ulcerative Colitis: Impact of intensified or accelerated infliximab dosing Open
Background The role of intensified (>5mg/kg), accelerated (>1 dose within first 14 days) infliximab (IFX) as rescue therapy in patients with acute severe ulcerative colitis (ASUC) remains controversial. This project evaluated the ass…
View article: Case report: Refractory Evans syndrome in two patients with spondyloenchondrodysplasia with immune dysregulation treated successfully with JAK1/JAK2 inhibition
Case report: Refractory Evans syndrome in two patients with spondyloenchondrodysplasia with immune dysregulation treated successfully with JAK1/JAK2 inhibition Open
Biallelic mutations in the ACP5 gene cause spondyloenchondrodysplasia with immune dysregulation (SPENCDI). SPENCDI is characterized by the phenotypic triad of skeletal dysplasia, innate and adaptive immune dysfunction, and variable neurolo…
View article: γδ T cell antigen receptor polyspecificity enables T cell responses to a broad range of immune challenges
γδ T cell antigen receptor polyspecificity enables T cell responses to a broad range of immune challenges Open
γδ T cells are essential for immune defense and modulating physiological processes. While they have the potential to recognize large numbers of antigens through somatic gene rearrangement, the antigens which trigger most γδ T cell response…
View article: Single-cell transcriptomics reveal different maturation stages and sublineage commitment of human thymic invariant natural killer T cells
Single-cell transcriptomics reveal different maturation stages and sublineage commitment of human thymic invariant natural killer T cells Open
Invariant natural killer T cells are a rare, heterogeneous T-cell subset with cytotoxic and immunomodulatory properties. During thymic development, murine invariant natural killer T cells go through different maturation stages differentiat…
View article: Single-cell Transcriptomics Reveal Different Maturation Stages and Sublineages Commitment of Human Thymic Invariant Natural Killer T cells
Single-cell Transcriptomics Reveal Different Maturation Stages and Sublineages Commitment of Human Thymic Invariant Natural Killer T cells Open
Summary Invariant natural killer T (iNKT) cells are a subset of heterogenous T-cells with potent cytotoxic and immunomodulatory properties. During thymic development, murine iNKT cells go through different maturation stages and differentia…
View article: Sequential Stem Cell–Kidney Transplantation in Schimke Immuno-osseous Dysplasia
Sequential Stem Cell–Kidney Transplantation in Schimke Immuno-osseous Dysplasia Open
Lifelong immunosuppression is required for allograft survival after kidney transplantation but may not ultimately prevent allograft loss resulting from chronic rejection. We developed an approach that attempts to abrogate immune rejection …
View article: Increased double-negative αβ+ T-cells reveal adult-onset autoimmune lymphoproliferative syndrome in a patient with IgG4-related disease
Increased double-negative αβ+ T-cells reveal adult-onset autoimmune lymphoproliferative syndrome in a patient with IgG4-related disease Open
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View article: Single-Cell Analysis of the Neonatal Immune System Across the Gestational Age Continuum
Single-Cell Analysis of the Neonatal Immune System Across the Gestational Age Continuum Open
Although most causes of death and morbidity in premature infants are related to immune maladaptation, the premature immune system remains poorly understood. We provide a comprehensive single-cell depiction of the neonatal immune system at …
View article: Αβ T-Cell/CD19 B-Cell Depleted Haploidentical Stem Cell Transplantation: A New Platform for Curing Rare and Monogenic Disorders
Αβ T-Cell/CD19 B-Cell Depleted Haploidentical Stem Cell Transplantation: A New Platform for Curing Rare and Monogenic Disorders Open
Allogeneic hematopoietic stem cell transplantation (HSCT) from an HLA-matched donor, either related or unrelated, has been extensively used to treat patients with genetic disorders. However, only 25% of patients eligible to receive allogen…
View article: Durable multitransgene expression in vivo using systemic, nonviral DNA delivery
Durable multitransgene expression in vivo using systemic, nonviral DNA delivery Open
HEDGES, a tunable nonviral DNA-based gene therapy platform, durably expresses human therapeutic proteins in immunocompetent mice.
View article: Multiomics modeling of the immunome, transcriptome, microbiome, proteome and metabolome adaptations during human pregnancy
Multiomics modeling of the immunome, transcriptome, microbiome, proteome and metabolome adaptations during human pregnancy Open
Motivation Multiple biological clocks govern a healthy pregnancy. These biological mechanisms produce immunologic, metabolomic, proteomic, genomic and microbiomic adaptations during the course of pregnancy. Modeling the chronology of these…
View article: An immune clock of human pregnancy
An immune clock of human pregnancy Open
High-dimensional analysis reveals the chronology of immunological adaptations during the course of human pregnancies.
View article: Novel nonsense gain-of-function NFKB2 mutations associated with a combined immunodeficiency phenotype
Novel nonsense gain-of-function NFKB2 mutations associated with a combined immunodeficiency phenotype Open
Key Points NFKB2 GOF mutations are associated with CID without endocrine or ectodermal manifestations. As most autosomal-dominant primary immunodeficiencies, NFKB2 GOF changes have incomplete penetrance and variable expressivity.
View article: A Case Report of Hypoglycemia and Hypogammaglobulinemia: DAVID Syndrome in a Patient With a Novel <i>NFKB2</i> Mutation
A Case Report of Hypoglycemia and Hypogammaglobulinemia: DAVID Syndrome in a Patient With a Novel <i>NFKB2</i> Mutation Open
Context: Deficient anterior pituitary with variable immune deficiency (DAVID) syndrome is a rare disorder in which children present with symptomatic adrenocorticotropic hormone (ACTH) deficiency preceded by hypogammaglobulinemia from B-cel…
View article: Expression of DNA integrated into flagellar region.
Expression of DNA integrated into flagellar region. Open
(A) The relative expression levels of fliD, fliS, fliT, and fliY compared to the mean transcription of the two house-keeping genes arcA and rpoD, where each of the housekeeping genes was assayed in triplicate (H). Experiments were carried …
View article: Location and expression of the <i>E. coli</i> flagellar regions.
Location and expression of the <i>E. coli</i> flagellar regions. Open
The E. coli K12 MG1655 genome showing positions of macrodomains (RIGHT, TER, LEFT, and ORI) and RNA polymerase (RNA-POL) binding (green peaks; ChIP-seq data from cells at mid-exponential growth phase [10]). E. coli flagellar genes are amon…
View article: Mapping the Fetomaternal Peripheral Immune System at Term Pregnancy
Mapping the Fetomaternal Peripheral Immune System at Term Pregnancy Open
Preterm labor and infections are the leading causes of neonatal deaths worldwide. During pregnancy, immunological cross talk between the mother and her fetus is critical for the maintenance of pregnancy and the delivery of an immunocompete…