David Baddeley
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View article: A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context
A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context Open
Fluorescence microscopy has been widely used to reveal the spatial distribution of specifically labeled molecules, but it is blind to cellular context. Quantitative phase contrast microscopy (QPC) provides such complementary information. H…
View article: Resolution in super-resolution microscopy – facts, artifacts, technological advancements and biological applications
Resolution in super-resolution microscopy – facts, artifacts, technological advancements and biological applications Open
Super-resolution microscopy (SRM) has undeniable potential for scientific discovery, yet still presents many challenges that hinder its widespread adoption, including technical trade-offs between resolution, speed and photodamage, as well …
View article: Electrophysiology and 3D‐imaging reveal properties of human intracardiac neurons and increased excitability with atrial fibrillation
Electrophysiology and 3D‐imaging reveal properties of human intracardiac neurons and increased excitability with atrial fibrillation Open
Altered autonomic input to the heart plays a major role in atrial fibrillation (AF). Autonomic neurons termed ganglionated plexi (GP) are clustered on the heart surface to provide the last point of neural control of cardiac function. To da…
View article: The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ
The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ Open
The endoplasmic reticulum’s (ER’s) structure is directly linked to the many functions of the ER, but its formation is not fully understood. We investigate how the ER–membrane curving protein reticulon 4 (Rtn4) localizes to and organizes in…
View article: Extracting nanoscale membrane morphology from single-molecule localizations
Extracting nanoscale membrane morphology from single-molecule localizations Open
Membrane surface reconstruction at the nanometer scale is required for understanding mechanisms of subcellular shape change. This historically has been the domain of electron microscopy, but extraction of surfaces from specific labels is a…
View article: Extracting nanoscale membrane morphology from single-molecule localizations
Extracting nanoscale membrane morphology from single-molecule localizations Open
Membrane surface reconstruction at the nanometer scale is required for understanding mechanisms of subcellular shape change. This historically has been the domain of electron microscopy, but extraction of surfaces from specific labels is a…
View article: The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure<i>in situ</i>
The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure<i>in situ</i> Open
The endoplasmic reticulum’s (ER) structure is directly linked to the many functions of the ER but its formation is not fully understood. We investigate how the ER-membrane curving protein reticulon 4 (Rtn4) localizes to and organizes in th…
View article: The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ
The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ Open
Super-resolution microscopy data described in the publication of the same title.
View article: Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging
Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging Open
Data associated with publication.
View article: Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging
Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging Open
Data associated with publication.
View article: Optimal Detection of Fusion Pore Dynamics Using Polarized Total Internal Reflection Fluorescence Microscopy
Optimal Detection of Fusion Pore Dynamics Using Polarized Total Internal Reflection Fluorescence Microscopy Open
The fusion pore is the initial narrow connection that forms between fusing membranes. During vesicular release of hormones or neurotransmitters, the nanometer-sized fusion pore may open-close repeatedly (flicker) before resealing or dilati…
View article: Nanoscale Organisation of Ryanodine Receptors and Junctophilin-2 in the Failing Human Heart
Nanoscale Organisation of Ryanodine Receptors and Junctophilin-2 in the Failing Human Heart Open
The disrupted organisation of the ryanodine receptors (RyR) and junctophilin (JPH) is thought to underpin the transverse tubule (t-tubule) remodelling in a failing heart. Here, we assessed the nanoscale organisation of these two key protei…
View article: python-microscopy/python-microscopy: Release 20.11.25
python-microscopy/python-microscopy: Release 20.11.25 Open
The python-microscopy environment - an open-source package for light microscopy
View article: PYMEVisualize: an open-source tool for exploring 3D super-resolution data
PYMEVisualize: an open-source tool for exploring 3D super-resolution data Open
Localization-based super-resolution microscopy techniques such as PALM, STORM, and PAINT are increasingly critical tools for biological discovery. These methods generate lists of single fluorophore positions that capture nanoscale structur…
View article: Fluorogenic probe for fast 3D whole-cell DNA-PAINT
Fluorogenic probe for fast 3D whole-cell DNA-PAINT Open
DNA-PAINT is an increasingly popular super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and very slow imaging speed, both of which can be attributed t…
View article: Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis
Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis Open
Actin dynamics generate forces to deform the membrane and overcome the cell’s high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that …
View article: Spontaneous Activity Patterns Are Altered in the Developing Visual Cortex of the Fmr1 Knockout Mouse
Spontaneous Activity Patterns Are Altered in the Developing Visual Cortex of the Fmr1 Knockout Mouse Open
Fragile X syndrome (FXS) is the most prevalent inherited cause of autism and is accompanied by behavioral and sensory deficits. Errors in the wiring of the brain during early development likely contribute to these deficits, but the underly…
View article: 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples
3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples Open
Assessment of the imaging quality in localisation-based super-resolution techniques relies on an accurate characterisation of the imaging setup and analysis procedures. Test samples can provide regular feedback on system performance and fa…
View article: 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples
3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples Open
Assessment of the imaging quality in localisation-based super-resolution techniques relies on an accurate characterisation of the imaging setup and analysis procedures. Test samples can provide regular feedback on system performance and fa…
View article: Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis
Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis Open
Actin is required for clathrin-mediated endocytosis (CME) in yeast. Experimental observations indicate that this actin assembly generates force to deform the membrane and overcome the cell’s high turgor pressure, but the precise molecular …