David Bartee
YOU?
Author Swipe
View article: Molecular Basis for RNA Cytidine Acetylation by NAT10
Molecular Basis for RNA Cytidine Acetylation by NAT10 Open
Human NAT10 acetylates the N4 position of cytidine in RNA, predominantly on rRNA and tRNA, to facilitate ribosome biogenesis and protein translation. NAT10 has been proposed as a therapeutic target in cancers as well as aging-associated pa…
View article: Investigating inhibitors of 1-deoxy- <scp>d</scp> -xylulose 5-phosphate synthase in a mouse model of UTI
Investigating inhibitors of 1-deoxy- <span>d</span> -xylulose 5-phosphate synthase in a mouse model of UTI Open
The rising rate of antimicrobial resistance continues to threaten global public health. Further hastening antimicrobial resistance is the lack of new antibiotics against new targets. The bacterial enzyme, 1-deoxy- d -xylulose 5-phosphate s…
View article: PI3K drives the de novo synthesis of coenzyme A from vitamin B5
PI3K drives the de novo synthesis of coenzyme A from vitamin B5 Open
In response to hormones and growth factors, the class I phosphoinositide-3-kinase (PI3K) signalling network functions as a major regulator of metabolism and growth, governing cellular nutrient uptake, energy generation, reducing cofactor p…
View article: Site-Specific Synthesis of <i>N</i> <sup>4</sup> -Acetylcytidine in RNA Reveals Physiological Duplex Stabilization
Site-Specific Synthesis of <i>N</i> <sup>4</sup> -Acetylcytidine in RNA Reveals Physiological Duplex Stabilization Open
N4-Acetylcytidine (ac4C) is a post-transcriptional modification of RNA that is conserved across all domains of life. All characterized sites of ac4C in eukaryotic RNA occur in the central nucleotide of a 5'-CCG-3' consensus sequence. Howev…
View article: Site-Specific Synthesis of N4-Acetylcytidine in RNA Reveals Physiological Duplex Stabilization
Site-Specific Synthesis of N4-Acetylcytidine in RNA Reveals Physiological Duplex Stabilization Open
N 4 -acetylcytidine (ac4C) is a post-transcriptional modification of RNA that is conserved across all domains of life. All characterized sites of ac4C in eukaryotic RNA occur in the central nucleotide of a 5’-CCG-3’ consensus sequence. How…
View article: Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues
Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues Open
Lysine lactoylation is a recently described protein post-translational modification (PTM). However, the biochemical pathways responsible for this acylation remain unclear. Two metabolite-dependent mechanisms have been proposed: enzymatic h…
View article: Direct anabolic three carbon metabolism via propionate to a six carbon metabolite occurs in human heart and in vivo across mouse tissues
Direct anabolic three carbon metabolism via propionate to a six carbon metabolite occurs in human heart and in vivo across mouse tissues Open
Anabolic metabolism of carbon in mammals is mediated via the one and two carbon carriers S-adenosyl methionine and acetyl-coenzyme A (acetyl-CoA). In contrast, anabolic metabolism using three carbon units via propionate is not thought to o…
View article: Toward Understanding the Chemistry and Biology of 1-Deoxy-<scp>d</scp>-xylulose 5-Phosphate (DXP) Synthase: A Unique Antimicrobial Target at the Heart of Bacterial Metabolism
Toward Understanding the Chemistry and Biology of 1-Deoxy-<span>d</span>-xylulose 5-Phosphate (DXP) Synthase: A Unique Antimicrobial Target at the Heart of Bacterial Metabolism Open
Antibiotics are the cornerstone of modern healthcare. The 20th century discovery of sulfonamides and β-lactam antibiotics altered human society immensely. Simple bacterial infections were no longer a leading cause of morbidity and mortalit…
View article: Growth medium-dependent antimicrobial activity of early stage MEP pathway inhibitors
Growth medium-dependent antimicrobial activity of early stage MEP pathway inhibitors Open
The in vivo microenvironment of bacterial pathogens is often characterized by nutrient limitation. Consequently, conventional rich in vitro culture conditions used widely to evaluate antibacterial agents are often poorly predictive of in v…
View article: A novel lipoate attachment enzyme is shared by Plasmodium and Chlamydia species
A novel lipoate attachment enzyme is shared by Plasmodium and Chlamydia species Open
Summary Lipoate is an essential cofactor for enzymes that are important for central metabolism and other processes. In malaria parasites, scavenged lipoate from the human host is required for survival. The Plasmodium falciparum mitochondri…
View article: Challenges and Hallmarks of Establishing Alkylacetylphosphonates as Probes of Bacterial 1-Deoxy-<scp>d</scp>-xylulose 5-Phosphate Synthase
Challenges and Hallmarks of Establishing Alkylacetylphosphonates as Probes of Bacterial 1-Deoxy-<span>d</span>-xylulose 5-Phosphate Synthase Open
1-Deoxy-d-xylulose 5-phosphate (DXP) synthase catalyzes the thiamin diphosphate (ThDP)-dependent formation of DXP from pyruvate and d-glyceraldehyde 3-phosphate. DXP is at a metabolic branch point in bacteria, feeding into the methylerythr…