David Buhrke
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View article: Data for Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3
Data for Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3 Open
Data used in the Manuscript Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3
View article: Data for Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3
Data for Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3 Open
Data used in the Manuscript Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3
View article: Data for "The Molecular Mechanism of Light-induced Bond Formation and Breakage in the Cyanobacteriochrome TePixJ"
Data for "The Molecular Mechanism of Light-induced Bond Formation and Breakage in the Cyanobacteriochrome TePixJ" Open
Data used to generate the figures in the publication "The Molecular Mechanism of Light-induced Bond Formation and Breakage in the Cyanobacteriochrome TePixJ".
View article: Data for "The Molecular Mechanism of Light-induced Bond Formation and Breakage in the Cyanobacteriochrome TePixJ"
Data for "The Molecular Mechanism of Light-induced Bond Formation and Breakage in the Cyanobacteriochrome TePixJ" Open
Data used to generate the figures in the publication "The Molecular Mechanism of Light-induced Bond Formation and Breakage in the Cyanobacteriochrome TePixJ".
View article: Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3
Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3 Open
Cyanobacteriochromes are bi-stable photoreceptor proteins with desirable photochemical properties for biotechnological applications such as optogenetics or fluorescence microscopy. Here, we investigated Slr1393-g3, a cyanobacteriochrome th…
View article: The molecular mechanism of light-induced bond formation and breakage in the cyanobacteriochrome TePixJ
The molecular mechanism of light-induced bond formation and breakage in the cyanobacteriochrome TePixJ Open
The photoreaction of the cyanobacteriochrome TePixJ was studied with advanced IR-spectroscopic methods to gain insights into the molecular mechanism of its intricate photochemistry.
View article: Vibrational couplings between protein and cofactor in bacterial phytochrome Agp1 revealed by 2D-IR spectroscopy
Vibrational couplings between protein and cofactor in bacterial phytochrome Agp1 revealed by 2D-IR spectroscopy Open
Phytochromes are ubiquitous photoreceptor proteins that undergo a significant refolding of secondary structure in response to initial photoisomerization of the chromophoric group. This process is important for the signal transduction throu…
View article: Data for Vibrational Couplings between Protein and Co-factor in Bacterial Phytochrome Agp1revealed by 2D-IR Spectroscopy
Data for Vibrational Couplings between Protein and Co-factor in Bacterial Phytochrome Agp1revealed by 2D-IR Spectroscopy Open
2D-IR data for the bacteriophytochrome Agp1 in the Pr and Pfr states
View article: Data for Vibrational Couplings between Protein and Co-factor in Bacterial Phytochrome Agp1revealed by 2D-IR Spectroscopy
Data for Vibrational Couplings between Protein and Co-factor in Bacterial Phytochrome Agp1revealed by 2D-IR Spectroscopy Open
2D-IR data for the bacteriophytochrome Agp1 in the Pr and Pfr states
View article: Signal Propagation Within the MCL-1/BIM Protein Complex
Signal Propagation Within the MCL-1/BIM Protein Complex Open
The protein MCL-1 is a crucial factor in regulating apoptosis, the programmed cell death, and thus plays a major role in numerous cancer types. The allosteric protein MCL-1 is naturally moderated by the BH3-only peptide BIM, which binds at…
View article: A stop-flow sample delivery system for transient spectroscopy
A stop-flow sample delivery system for transient spectroscopy Open
A stop-flow sample delivery system for transient spectroscopy is presented, which is, in particular, suited for laser-based instruments (quantum-cascade lasers or amplified femtosecond lasers) with excitation pulse repetition rates in the …
View article: On the Role of the Conserved Histidine at the Chromophore Isomerization Site in Phytochromes
On the Role of the Conserved Histidine at the Chromophore Isomerization Site in Phytochromes Open
Phytochromes are sensory photoreceptors that use light to drive protein structural changes, which in turn trigger physiological reaction cascades. The process starts with a double-bond photoisomerization of the linear methine-bridged tetra…
View article: Data for "The Impact of Chromophore Choice on the Assembly Kinetics and Primary Photochemistry of a red/green Cyanobacteriochrome"
Data for "The Impact of Chromophore Choice on the Assembly Kinetics and Primary Photochemistry of a red/green Cyanobacteriochrome" Open
Data for the publication "The Impact of Chromophore Choice on the Assembly Kinetics and Primary Photochemistry of a red/green Cyanobacteriochrome" in PCCP
View article: Data for "The Impact of Chromophore Choice on the Assembly Kinetics and Primary Photochemistry of a red/green Cyanobacteriochrome"
Data for "The Impact of Chromophore Choice on the Assembly Kinetics and Primary Photochemistry of a red/green Cyanobacteriochrome" Open
Data for the publication "The Impact of Chromophore Choice on the Assembly Kinetics and Primary Photochemistry of a red/green Cyanobacteriochrome" in PCCP
View article: Sequence of Events during Peptide Unbinding from RNase S: A Complete Experimental Description
Sequence of Events during Peptide Unbinding from RNase S: A Complete Experimental Description Open
The photo-triggered unbinding of the intrinsically disordered S-peptide from the RNase S complex is studied with the help of transient IR spectroscopy, covering a wide range of time scales from 100 ps to 10 ms. To that end, an azobenzene m…
View article: The Speed of Allosteric Signaling Within a Single-Domain Protein
The Speed of Allosteric Signaling Within a Single-Domain Protein Open
While much is known about different allosteric regulation mechanisms, the nature of the "allosteric signal", and the timescale on which it propagates, remains elusive. The PDZ3 domain from postsynaptic density-95 protein is a small protein…
View article: The impact of chromophore choice on the assembly kinetics and primary photochemistry of a red/green cyanobacteriochrome
The impact of chromophore choice on the assembly kinetics and primary photochemistry of a red/green cyanobacteriochrome Open
The assembly reaction and primary photochemistry of a cyanobacteriochrome photoreceptor with two different chromophores were investigated with transient absorption spectroscopy.
View article: Needles in a haystack: H-bonding in an optogenetic protein observed with isotope labeling and 2D-IR spectroscopy
Needles in a haystack: H-bonding in an optogenetic protein observed with isotope labeling and 2D-IR spectroscopy Open
Two vibrational modes in a cyanobacterial protein were isolated with isotope labeling and studied with 2D-IR spectroscopy.
View article: Light- and temperature-dependent dynamics of chromophore and protein structural changes in bathy phytochrome Agp2
Light- and temperature-dependent dynamics of chromophore and protein structural changes in bathy phytochrome Agp2 Open
Time-resolved vibrational spectroscopies reveal that phototransformation of the Pfr dark state of bacterial phytochrome Agp2 follows a branched mechanism with a productive and non-productive pathway.
View article: Nanosecond protein dynamics in a red/green cyanobacteriochrome revealed by transient IR spectroscopy
Nanosecond protein dynamics in a red/green cyanobacteriochrome revealed by transient IR spectroscopy Open
Over the last decades, photoreceptive proteins were extensively studied with biophysical methods to gain a fundamental understanding of their working mechanisms and further guide the development of optogenetic tools. Time-resolved infrared…
View article: Data for "Nanosecond Protein Dynamics in a Red/Green Cyanobacteriochrome Revealed by Transient IR Spectroscopy "
Data for "Nanosecond Protein Dynamics in a Red/Green Cyanobacteriochrome Revealed by Transient IR Spectroscopy " Open
Raw data behind manuscript figures.
View article: Data for "Nanosecond Protein Dynamics in a Red/Green Cyanobacteriochrome Revealed by Transient IR Spectroscopy "
Data for "Nanosecond Protein Dynamics in a Red/Green Cyanobacteriochrome Revealed by Transient IR Spectroscopy " Open
Raw data behind manuscript figures.
View article: Real-time observation of ligand-induced allosteric transitions in a PDZ domain
Real-time observation of ligand-induced allosteric transitions in a PDZ domain Open
Significance Allostery is a process in which a signal sensed upon ligand-binding at a distal site is transduced to the effector site, allowing for regulation of the activity of the latter. The propagation of an allosteric signal is a noneq…
View article: Data deposition
Data deposition Open
Data deposition files
View article: Data deposition
Data deposition Open
Data deposition files
View article: Distinct chromophore–protein environments enable asymmetric activation of a bacteriophytochrome-activated diguanylate cyclase
Distinct chromophore–protein environments enable asymmetric activation of a bacteriophytochrome-activated diguanylate cyclase Open
Sensing of red and far-red light by bacteriophytochromes involves intricate interactions between their bilin chromophore and the protein environment. The light-triggered rearrangements of the cofactor configuration and eventually the prote…
View article: Spectroscopic investigation of phytochromes and NIR fluorescent variants
Spectroscopic investigation of phytochromes and NIR fluorescent variants Open
In this work, vibrational spectroscopic methods (resonance Raman [RR] and infrared absorption [IR]) were used for the investigation of phytochromes. Phytochromes are bistable photoreceptor proteins that bind bilins (BV, PCB, PΦB) as chromo…