Explanipedia

Parapoxvirus causes a deleterious disease in red squirrels associated with UK population declines Open

Daniel M. Tompkins, Anthony W. Sainsbury, P. F. Nettleton, D. Buxton, John Gurnell · 2002

Biology Medicine

The disease implications of novel pathogens need to be considered when investigating the ecological impact of species translocations on native fauna. Traditional explanations based on competition or predation may often not be the whole sto…

Experimental Paratuberculosis in Calves following Inoculation with a Rabbit Isolate of Mycobacterium avium subsp. paratuberculosis Open

Philippa M. Beard, Karen Stevenson, A. Pirie, Karen Rudge, D. Buxton , et al. · 2001

Biology Medicine

The role of wildlife species in the epidemiology of paratuberculosis has been the subject of increased research efforts following the discovery of natural paratuberculosis in free-living rabbits from farms in east Scotland. This paper desc…

Chlamydial Infection in sheep: Immune Control versus Fetal Pathology Open

Gary Entrican, D. Buxton, David Longbottom · 2001

Biology

Chlamydial abortion was first described by Greig1 in 1936 and named enzootic abortion of ewes (EAE). At that time he suggested that it was the result of environmental factors such as dietary deficiency. It was not until 1950 that Stamp and…

Paratuberculosis Infection of Nonruminant Wildlife in Scotland Open

Philippa M. Beard, Mike Daniels, D. Henderson, A. Pirie, Karen Rudge , et al. · 2001

Biology Medicine

Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive s…

Examination of red foxes ( Vulpes vulpes ) from Belgium for antibody to Neospora caninum and Toxoplasma gondii Open

D. Buxton, S. Maley, Paul-Pierre Pastoret, B. Brochier, Elisabeth A. Innes · 1997

Medicine Biology Computer science

peer reviewed

Detection of Mycobacterium tuberculosis directly from sputum by using a prototype automated Q-beta replicase assay Open

James H. Smith, D. Buxton, P Cahill, Massimo S. Fiandaca, L Goldston , et al. · 1997

Medicine Biology Chemistry

We have adapted an assay for the direct detection of Mycobacterium tuberculosis using a prototype automated instrument platform in which probes are amplified with Q-beta replicase. The assay was based on amplification of specific detector …

Q-beta replicase-amplified assay for detection of Mycobacterium tuberculosis directly from clinical specimens Open

Jyotsna Shah, J Liu, D. Buxton, A Hendricks, Lacey B. Robinson , et al. · 1995

Biology Chemistry Medicine

We report the results of a study conducted to evaluate the performance of manual Q-Beta replicase-amplified Mycobacterium tuberculosis complex assay compared with that of culture for detecting M. tuberculosis directly from digested sputum …

Comparison of amplified Q beta replicase and PCR assays for detection of Mycobacterium tuberculosis Open

Qing An, D. Buxton, A Hendricks, Lacey B. Robinson, Jyotsna Shah , et al. · 1995

Biology Medicine

Because of the long time required to isolate Mycobacterium tuberculosis in culture, there is an acute need for simple rapid methods for direct detection of M. tuberculosis from human sputum specimens. We have developed and characterized qu…

Detection of Mycobacterium tuberculosis directly from spiked human sputum by Q-beta replicase-amplified assay Open

Jyotsna Shah, J Liu, D. Buxton, Barbara Stone, Raymond Nietupski , et al. · 1995

Biology Medicine

We report on a rapid, sensitive, Q-Beta replicase-amplified nucleic acid hybridization assay for the detection of Mycobacterium tuberculosis directly from spiked human sputum. Specimens were processed by either an N-acetyl-L-cysteine-NaOH …

Comparison of characteristics of Q beta replicase-amplified assay with competitive PCR assay for Chlamydia trachomatis Open

Qing An, J Liu, William J. O’Brien, G Radcliffe, D. Buxton , et al. · 1995

Biology Chemistry

In order to study infections due to Chlamydia trachomatis, we have compared semiquantitative PCR and Q beta replicase-amplified assays for detection of this organism. The PCR assay was directed against the C. trachomatis 16S rRNA gene. Qua…

The importance of interferon-gamma in an early infection of Chlamydia psittaci in mice. Open

Michael Campbell McCafferty, S. Maley, Gary Entrican, D. Buxton · 1994

Biology

Athymic mice (nu/nu) and their hairy littermates (nu/+) were infected experimentally with Chlamydia psittaci and the role of endogenous interferon-gamma (IFN-gamma) on the resolution of the infection was studied. The pathological changes p…

Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection Open

Qing An, G Radcliffe, Roberto Vassallo, D. Buxton, William J. O’Brien , et al. · 1992

Biology

Clinical samples in transport media from 40 patients exhibiting pathologies potentially caused by Chlamydia trachomatis infection were analyzed for chlamydial nucleic acid, and the results were compared with those of culture. Chlamydial cu…

Acute placentitis and spontaneous abortion caused by chlamydia psittaci of sheep origin: a histological and ultrastructural study. Open

Siew Yee Wong, Elizabeth Gray, D. Buxton, J. Finlayson, F. W. A. Johnson · 1985

Medicine Biology

A sheep farmer's wife who had been assisting with lambing developed an influenza like illness in the 28th week of pregnancy. After five days of malaise she spontaneously delivered a stillborn infant; she became acutely ill during the immed…

Effect of chronic Trypanosoma brucei infection on the course of louping ill virus infection in mice Open

H.W. Reid, D. Buxton, J. Finlayson, P.H. Holmes · 1979

Biology

The course of louping-ill virus infection in mice chronically infected with Trypanosoma brucei was investigated. The mean survival time of mice infected with virus alone was 10.2 days, compared to 12.8 days in mice infected with T. brucei …

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