David C. Muddiman
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View article: Signal enhancement of lipids and glycans using ammonium fluoride for IR-MALDESI mass spectrometry imaging
Signal enhancement of lipids and glycans using ammonium fluoride for IR-MALDESI mass spectrometry imaging Open
Mass spectrometry imaging (MSI) is an analytical technique that enables the simultaneous detection and localization of hundreds to thousands of molecules across a tissue surface. The addition of ammonium fluoride to the electrospray or to …
View article: Native mass spectrometry enabled by infrared matrix-assisted laser desorption electrospray ionization for rapid measurement of protein-ligand biophysical parameters
Native mass spectrometry enabled by infrared matrix-assisted laser desorption electrospray ionization for rapid measurement of protein-ligand biophysical parameters Open
The determination of an equilibrium dissociation constant ( K d ) alongside the maximum binding capacity ( B max ) of protein-ligand interactions is essential in understanding binding affinities and binding capacities which play a major ro…
View article: Exploring ammonium salt doping to enhance ion abundance for quantitative mass spectrometry imaging
Exploring ammonium salt doping to enhance ion abundance for quantitative mass spectrometry imaging Open
Increasing ion abundance in mass spectrometry is essential for enhancing detection, quantification, and understanding of biomolecules involved in key cellular processes. Infrared matrix-assisted laser desorption electrospray ionization (IR…
View article: Progress in monitoring molecular, cellular and metabolic dynamics using mass spectrometry imaging
Progress in monitoring molecular, cellular and metabolic dynamics using mass spectrometry imaging Open
Mass spectrometry imaging (MSI) enables the visualization of hundreds to thousands of analytes in biological tissues. MSI is also capable of mapping time-dependent processes that, combined with these static metabolite profiles, provides a …
View article: Demonstrating voxel-by-voxel (V × V) single-point calibration in liver tissue by IR-MALDESI quantitative MSI
Demonstrating voxel-by-voxel (V × V) single-point calibration in liver tissue by IR-MALDESI quantitative MSI Open
Quantitative mass spectrometry imaging (qMSI) provides information regarding the colocalization, relative abundance, and concentration of a target analyte in a tissue without homogenization. Ionization sources, including IR-MALDESI, common…
View article: A Strategy to Achieve Sub‐Parts‐per‐Million Mass Measurement Accuracy of <i>N</i>‐Linked Glycans Using Infrared Matrix‐Assisted Laser Desorption Electrospray Ionization
A Strategy to Achieve Sub‐Parts‐per‐Million Mass Measurement Accuracy of <i>N</i>‐Linked Glycans Using Infrared Matrix‐Assisted Laser Desorption Electrospray Ionization Open
Mass calibration techniques are vital in achieving high mass measurement accuracy (MMA) of large biomolecules. Variable ion populations that shift the axial frequencies due to space charge effects have been a significant challenge in achie…
View article: Comparative proteomic analysis of skin wound healing responses to biomaterial treatments identifies key pathways which govern differential regenerative outcomes
Comparative proteomic analysis of skin wound healing responses to biomaterial treatments identifies key pathways which govern differential regenerative outcomes Open
The wound healing cascade is characterized by the steady progression of distinct stages. Though biomaterials are used clinically to enhance wound closure rate and quality of healed tissue, their mechanisms of action are less understood. He…
View article: Unraveling the molecular dynamics of wound healing: integrating spatially resolved lipidomics and temporally resolved proteomics
Unraveling the molecular dynamics of wound healing: integrating spatially resolved lipidomics and temporally resolved proteomics Open
Understanding the spatial–temporal molecular dynamics of wound healing is crucial for devising effective treatments. Three-dimensional mass spectrometry imaging (3D MSI) enables the comprehensive visualization of molecular distribution thr…
View article: Comparison of Mid‐Infrared and Ultraviolet Lasers Coupled to the MALDESI Source for the Detection of Secondary Metabolites and Structural Lipids in <scp><i>Arabidopsis thaliana</i></scp>
Comparison of Mid‐Infrared and Ultraviolet Lasers Coupled to the MALDESI Source for the Detection of Secondary Metabolites and Structural Lipids in <span><i>Arabidopsis thaliana</i></span> Open
Matrix‐assisted laser desorption electrospray ionization (MALDESI) conventionally utilizes a mid‐infrared (IR) laser for the desorption of neutrals, allowing for detection of hundreds to thousands of analytes simultaneously. This platform …
View article: Part A: Implementing an Analyte Panel and Sampling Protocol for Quality Control in Mass Spectrometry Imaging
Part A: Implementing an Analyte Panel and Sampling Protocol for Quality Control in Mass Spectrometry Imaging Open
Rationale While quality control (QC) and system suitability testing (SST) methods are commonly employed in mass spectrometry, the field of mass spectrometry imaging (MSI) currently lacks any universally accepted QC/SST protocols. These met…
View article: Part B: SLICE‐MSI—A Machine Learning Interface for System Suitability Testing of Mass Spectrometry Imaging Platforms
Part B: SLICE‐MSI—A Machine Learning Interface for System Suitability Testing of Mass Spectrometry Imaging Platforms Open
Rationale The field of mass spectrometry imaging is currently devoid of standardized protocols or commercially available products designed for system suitability testing of MSI platforms. Machine learning is an approach that can quickly an…
View article: Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction
Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction Open
Leveraging a depth profiling approach expands the chemical elucidation of mass spectrometry imaging techniques to another dimension. Three-dimensional MSI (3D MSI) reveals the distribution of analytes with greater anatomical detail to add …
View article: Feasibility of IR‐MALDESI Mass Spectrometry Imaging of PFAS
Feasibility of IR‐MALDESI Mass Spectrometry Imaging of PFAS Open
Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are a class of emerging contaminants that have been in use industrially since the 1940s. Their long‐term and extensive commercial use has led to their ubiquitous presence in the environm…
View article: In-depth characterization of N-glycosylation and sialic acid content in fetal and adult fibrinogen
In-depth characterization of N-glycosylation and sialic acid content in fetal and adult fibrinogen Open
Sialylation in the Bβ chain of fetal fibrinogen supports previous findings showing more knob 'B' interactions occur in fetal fibrinogen than in adult fibrinogen during clot polymerization. This is also the first detection of glycosylation …
View article: Absolute Quantification of Glutathione Using Top‐Hat Optics for IR‐MALDESI Mass Spectrometry Imaging
Absolute Quantification of Glutathione Using Top‐Hat Optics for IR‐MALDESI Mass Spectrometry Imaging Open
Infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) uses an infrared laser to desorb neutral biomolecules with postionization via ESI at atmospheric pressure. The Gaussian profile of the laser with conventional o…
View article: Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI
Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI Open
Mass spectrometry imaging (MSI) has become a significant tool for measuring chemical species in biological tissues, where much of the impact of these platforms lies in their capability to report the spatial distribution of analytes for cor…
View article: Mass spectrometry imaging of <i>N</i>‐linked glycans: Fundamentals and recent advances
Mass spectrometry imaging of <i>N</i>‐linked glycans: Fundamentals and recent advances Open
With implications in several medical conditions, N ‐linked glycosylation is one of the most important posttranslation modifications present in all living organisms. Due to their nontemplate synthesis, glycan structures are extraordinarily …
View article: Improved detection in untargeted lipidomics through silver‐doped infrared matrix‐assisted laser desorption electrospray ionization
Improved detection in untargeted lipidomics through silver‐doped infrared matrix‐assisted laser desorption electrospray ionization Open
Rationale Silver doping of electrospray is known to increase the abundance of olefinic compounds detected by mass spectrometry. While demonstrated in targeted experiments, this has yet to be investigated in an untargeted study. Utilizing i…
View article: Quantitative mass spectrometry imaging (qMSI): A tutorial By Russell R. Kibbe and David C. Muddiman
Quantitative mass spectrometry imaging (qMSI): A tutorial By Russell R. Kibbe and David C. Muddiman Open
He holds a Bachelor of Science degree in chemistry (2021) from Wofford College in Spartanburg, South Carolina.His research involves fundamental investigations to improve the IR-MALDESI-
View article: Quantitative mass spectrometry imaging (qMSI): A tutorial
Quantitative mass spectrometry imaging (qMSI): A tutorial Open
Mass spectrometry imaging (MSI) is an analytical technique that enables the simultaneous detection of hundreds to thousands of chemical species while retaining their spatial information; usually, MSI is applied to biological tissues. Combi…
View article: A statistical approach to system suitability testing for mass spectrometry imaging
A statistical approach to system suitability testing for mass spectrometry imaging Open
Rationale Mass spectrometry imaging (MSI) elevates the power of conventional mass spectrometry (MS) to multidimensional space, elucidating both chemical composition and localization. However, the field lacks any robust quality control (QC)…
View article: Large-Scale Evaluation of Spatial Metabolomics Protocols and Technologies
Large-Scale Evaluation of Spatial Metabolomics Protocols and Technologies Open
Spatial metabolomics using imaging mass spectrometry (MS) enables untargeted and label-free metabolite mapping in biological samples. Despite the range of available imaging MS protocols and technologies, our understanding of metabolite det…
View article: RCM protocols: improving reproducibility in the field of mass spectrometry
RCM protocols: improving reproducibility in the field of mass spectrometry Open
The ability to repeatability and reproducibly replicate the outcomes of experiments is central to the scientific method. The corroboration of results by fellow scientists, either in another laboratory or within one's own, is what drives sc…
View article: Prototyping an ionization source for non‐engineers
Prototyping an ionization source for non‐engineers Open
Novel mass spectrometry (MS) based analytical platforms have enabled scientists to detect and quantify molecules within biological and environmental samples more accurately. Novel MS instrumentation starts as a prototype and, after years o…
View article: Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix‐assisted laser desorption electrospray ionization
Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix‐assisted laser desorption electrospray ionization Open
Rationale Infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) utilizes a 2970 nm mid‐IR laser to desorb samples with depth resolutions ( Z ) on the order of micrometers. Conventionally, 5–20 μm thick tissue secti…