David Elson
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View article: Long range RNA-RNA interactions in the 30 S ribosomal subunit of<i>E. coli</i>
Long range RNA-RNA interactions in the 30 S ribosomal subunit of<i>E. coli</i> Open
We have attempted to identify long-range interactions in the tertiary structure of RNA in the E. coli 30 S ribosome. Native subunits were cleaved with ribonuclease and separated into nucleoprotein fragments which were deproteinized and fra…
View article: The topology of the 30 S ribosomal subunit and a proposal for the surface distribution of its RNA by dark field electron microscopy.
The topology of the 30 S ribosomal subunit and a proposal for the surface distribution of its RNA by dark field electron microscopy. Open
A three-dimensional model of the Escherichia coli 30 S ribosome has been derived from an extensive dark field electron microscopic investigation of unstained and unshadowed ribosomes. Subsequent studies of specimens lightly stained with ur…
View article: A ribonucleoprotein fragment of the 30 S ribosome of<i>E. coli</i>containing two contiguous domains of the 16 S RNA
A ribonucleoprotein fragment of the 30 S ribosome of<i>E. coli</i>containing two contiguous domains of the 16 S RNA Open
Ribonucleoprotein fragments of the 30 S ribosome of E. coli have been prepared by limited ribonuclease digestion and mild heating of the ribosome in a constant ionic environment. One such fragment has been described previously. A second el…
View article: A ribonucleoprotein fragment of the 30 S ribosome of E. coli: evidence for long range RNA-RNA interactions
A ribonucleoprotein fragment of the 30 S ribosome of E. coli: evidence for long range RNA-RNA interactions Open
A stable homogeneous ribonucleoprotein fragment of the 30 S ribosomal subunit of E. coli has been prepared by mild nuclease digestion and heating in a constant ionic environment. The fragment contains about half of the 16 S ribosomal RNa a…
View article: Binding of magnesium ions and ethidium bromide: comparison of ribosomes and free ribosomal RNA
Binding of magnesium ions and ethidium bromide: comparison of ribosomes and free ribosomal RNA Open
Comparative studies of free ribosomal RNA and ribosomes were made with two probes, Mg++ ions and ethidium bromide, which interact with RNA in different ways. Mg++. E. coli 16 S rRNA and 30 S ribosomes were equilibrated with four different …
View article: A photoaffinity labelling study of the messenger RNA-binding region of Escherichia coli ribosomes
A photoaffinity labelling study of the messenger RNA-binding region of Escherichia coli ribosomes Open
A photoaffinity labelling study of the messenger RNA-binding region of E. coli ribosomes has been made, using oligoadenylic acids as mRNA analogs. The oligonucleotides, of chain length 6 to 8 and thus several nucleotides longer than oligon…
View article: The Use of a Cleavable Crosslinking Reagent to Identify Neighboring Proteins in the 30‐S Ribosomal Subunit of <i>Escherichia coli</i>
The Use of a Cleavable Crosslinking Reagent to Identify Neighboring Proteins in the 30‐S Ribosomal Subunit of <i>Escherichia coli</i> Open
A cleavable bifunctional reagent, dimethyl 3,3′‐dithiobispropionimidate, has been used to crosslink proteins that occupy neighboring positions in the 30‐S ribosomal subunit of Escherichia coli . The crosslinked proteins were identified, fu…
View article: The Protection by 70 S Ribosomes of <i>N</i>‐Acyl‐aminoacyl‐tRNA against Cleavage by Peptidyl‐tRNA Hydrolase and its Use to Assay Ribosomal Association
The Protection by 70 S Ribosomes of <i>N</i>‐Acyl‐aminoacyl‐tRNA against Cleavage by Peptidyl‐tRNA Hydrolase and its Use to Assay Ribosomal Association Open
Free N ‐carbobenzyloxy‐phenylalanyl‐tRNA is rapidly cleaved by the enzyme peptidyl‐tRNA hydrolase. When bound to a 30 S ribosome in the presence of poly(U), the substrate is hydrolyzed as rapidly as when free. The addition of 50 S ribosoma…
View article: On the specificity and stability of an enzyme that hydrolyzes N-substituted aminoacyl-transfer RNA's.
On the specificity and stability of an enzyme that hydrolyzes N-substituted aminoacyl-transfer RNA's. Open
In an earlier investigation, we observed that Cbz-phe-tRNA' was rapidly hydrolyzed by a crude supernatant fraction from E. coli, whereas unsubstituted phe-tRNA was relatively stable under the same conditions.2Subsequent studies, reported h…
View article: A RIBOSOME-CATALYZED REACTION BETWEEN N-FORMYLMETHIONYL-TRNA AND PUROMYCIN
A RIBOSOME-CATALYZED REACTION BETWEEN N-FORMYLMETHIONYL-TRNA AND PUROMYCIN Open
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social s…