David Furkert
YOU?
Author Swipe
View article: NUDIX hydrolases target specific inositol pyrophosphates and regulate phosphate homeostasis and bacterial pathogen susceptibility in Arabidopsis
NUDIX hydrolases target specific inositol pyrophosphates and regulate phosphate homeostasis and bacterial pathogen susceptibility in Arabidopsis Open
Inositol pyrophosphates (PP‐InsPs) are important signaling molecules that regulate diverse cellular processes in eukaryotes, including energy homeostasis, phosphate (P i ) signaling, and phytohormone perception. Yet, in plants, the enzymes…
View article: Inhibiting IP6K1 confers atheroprotection by elevating circulating apolipoprotein A-I
Inhibiting IP6K1 confers atheroprotection by elevating circulating apolipoprotein A-I Open
View article: Proteome-wide quantification of inositol pyrophosphate-protein interactions
Proteome-wide quantification of inositol pyrophosphate-protein interactions Open
Inositol polyphosphates (InsPs) and inositol pyrophosphates (PP-InsPs) constitute a group of highly phosphorylated molecules that are involved in many cellular signaling processes. To characterize discrete signaling events of these structu…
View article: Structural basis of dual BACH1 regulation by SCF<sup>FBXO22</sup>and SCF<sup>FBXL17</sup>
Structural basis of dual BACH1 regulation by SCF<sup>FBXO22</sup>and SCF<sup>FBXL17</sup> Open
Summary BTB and CNC homolog 1 (BACH1) is a master transcriptional regulator of the cellular oxidative stress response and pro-metastatic oncogene. Post-translational stability of BACH1 is tightly regulated by distinct F-box ubiquitin ligas…
View article: mRNA Display Identifies Potent, Paralog-Selective Peptidic Ligands for ARID1B
mRNA Display Identifies Potent, Paralog-Selective Peptidic Ligands for ARID1B Open
The ARID1A and ARID1B subunits are mutually exclusive components of the BAF variant of SWI/SNF chromatin remodeling complexes. Loss of function mutations in ARID1A are frequently observed in various cancers, resulting in a dependency on th…
View article: Depleting inositol pyrophosphate 5-InsP7 protected the heart against ischaemia–reperfusion injury by elevating plasma adiponectin
Depleting inositol pyrophosphate 5-InsP7 protected the heart against ischaemia–reperfusion injury by elevating plasma adiponectin Open
Aims Adiponectin is an adipocyte-derived circulating protein that exerts cardiovascular and metabolic protection. Due to the futile degradation of endogenous adiponectin and the challenges of exogenous administration, regulatory mechanisms…
View article: Itraconazole inhibits endothelial cell migration by disrupting inositol pyrophosphate-dependent focal adhesion dynamics and cytoskeletal remodeling
Itraconazole inhibits endothelial cell migration by disrupting inositol pyrophosphate-dependent focal adhesion dynamics and cytoskeletal remodeling Open
The antifungal drug itraconazole has been repurposed to anti-angiogenic agent, but the mechanisms of action have been elusive. Here we report that itraconazole disrupts focal adhesion dynamics and cytoskeletal remodeling, which requires 5-…
View article: Versatile signaling mechanisms of inositol pyrophosphates
Versatile signaling mechanisms of inositol pyrophosphates Open
Inositol pyrophosphates (PP-InsPs) constitute a group of highly charged messengers, which regulate central biological processes in health and disease, such as cellular phosphate and general energy homeostasis. Deciphering the molecular mec…
View article: Structural evidence for visual arrestin priming via complexation of phosphoinositols
Structural evidence for visual arrestin priming via complexation of phosphoinositols Open
View article: Using Biotinylated <i>myo</i>-Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate–Protein Interactions with Surface-Based Biosensors
Using Biotinylated <i>myo</i>-Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate–Protein Interactions with Surface-Based Biosensors Open
Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins to regulate biological functions, such a…
View article: Affinity enrichment and identification of inositol poly- and pyrophosphate interactomes
Affinity enrichment and identification of inositol poly- and pyrophosphate interactomes Open
This protocol describes an affinity enrichment approach from mammalian cell extracts to identify protein binding partners of inositol hexakisphosphate (InsP6) and 5-diphosphoinositol pentakisphosphate (5PP-InsP5), two…
View article: Dissecting the activation of insulin degrading enzyme by inositol pyrophosphates and their bisphosphonate analogs
Dissecting the activation of insulin degrading enzyme by inositol pyrophosphates and their bisphosphonate analogs Open
An efficient synthesis of non-hydrolyzable inositol pyrophosphate analogs was developed to subsequently investigate the regulation of insulin-degrading enzyme by these hyperphosphorylated signaling molecules.
View article: The inositol pyrophosphate 5-InsP <sub>7</sub> drives sodium-potassium pump degradation by relieving an autoinhibitory domain of PI3K p85α
The inositol pyrophosphate 5-InsP <sub>7</sub> drives sodium-potassium pump degradation by relieving an autoinhibitory domain of PI3K p85α Open
5-InsP 7 is an endogenous negative regulator of Na + /K + -ATPase.
View article: IP<sub>7</sub>-SPX Domain Interaction Controls Fungal Virulence by Stabilizing Phosphate Signaling Machinery
IP<sub>7</sub>-SPX Domain Interaction Controls Fungal Virulence by Stabilizing Phosphate Signaling Machinery Open
Invasive fungal diseases pose a serious threat to human health globally with >1.5 million deaths occurring annually, 180,000 of which are attributable to the AIDS-related pathogen, Cryptococcus neoformans . Here, we demonstrate that intera…
View article: Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome
Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome Open