David Giacalone
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View article: The phospho-ferrozine assay: a tool to study bacterial redox-active metabolites produced at the plant root
The phospho-ferrozine assay: a tool to study bacterial redox-active metabolites produced at the plant root Open
Soil microbial communities are pivotal to plant health and nutrient acquisition. It is becoming increasingly clear that many interactions, both among and between microbes and plants, are governed by small bioactive molecules or “secondary …
View article: Single-spore germination analyses reveal that calcium released during <i>Clostridioides difficile</i> germination functions in a feedforward loop
Single-spore germination analyses reveal that calcium released during <i>Clostridioides difficile</i> germination functions in a feedforward loop Open
Clostridioides difficile infections begin when its metabolically dormant spores germinate in response to sensing bile acid germinants alongside amino acid and divalent cation co-germinants in the small intestine. While bile acid germinants…
View article: Single-spore germination analyses reveal that calcium released during<i>Clostridioides difficile</i>germination functions in a feed-forward loop
Single-spore germination analyses reveal that calcium released during<i>Clostridioides difficile</i>germination functions in a feed-forward loop Open
Clostridioides difficile infections begin when its metabolically dormant spores germinate in response to sensing bile acid germinants alongside amino acid and divalent cation co-germinants in the small intestine. While bile acid germinants…
View article: PrrA modulates Mycobacterium tuberculosis response to multiple environmental cues and is critically regulated by serine/threonine protein kinases
PrrA modulates Mycobacterium tuberculosis response to multiple environmental cues and is critically regulated by serine/threonine protein kinases Open
The ability of Mycobacterium tuberculosis (Mtb) to adapt to its surrounding environment is critical for the bacterium to successfully colonize its host. Transcriptional changes are a vital mechanism by which Mtb responds to key environment…
View article: PrrA modulates <i>Mycobacterium tuberculosis</i> response to multiple environmental cues and is critically regulated by serine/threonine protein kinases
PrrA modulates <i>Mycobacterium tuberculosis</i> response to multiple environmental cues and is critically regulated by serine/threonine protein kinases Open
The ability of Mycobacterium tuberculosis (Mtb) to adapt to its surrounding environment is critical for the bacterium to successfully colonize its host. Transcriptional changes are a vital mechanism by which Mtb responds to key environment…
View article: <scp>Rv0500A</scp> is a transcription factor that links <i>Mycobacterium tuberculosis</i> environmental response with division and impacts host colonization
<span>Rv0500A</span> is a transcription factor that links <i>Mycobacterium tuberculosis</i> environmental response with division and impacts host colonization Open
For Mycobacterium tuberculosis (Mtb) to successfully infect a host, it must be able to adapt to changes in its microenvironment, including variations in ionic signals such as pH and chloride (Cl − ), and link these responses to its growth.…
View article: Targeting Mycobacterium tuberculosis response to environmental cues for the development of effective antitubercular drugs
Targeting Mycobacterium tuberculosis response to environmental cues for the development of effective antitubercular drugs Open
Sensing and response to environmental cues, such as pH and chloride (Cl − ), is critical in enabling Mycobacterium tuberculosis (Mtb) colonization of its host. Utilizing a fluorescent reporter Mtb strain in a chemical screen, we have ident…
View article: Role of SpoIVA ATPase Motifs during Clostridioides difficile Sporulation
Role of SpoIVA ATPase Motifs during Clostridioides difficile Sporulation Open
The major pathogen Clostridioides difficile depends on its spore form to transmit disease. However, the mechanism by which C. difficile assembles spores remains poorly characterized. We previously showed that binding between the spore morp…
View article: Role of SpoIVA ATPase Motifs During<i>Clostridioides difficile</i>Sporulation
Role of SpoIVA ATPase Motifs During<i>Clostridioides difficile</i>Sporulation Open
The nosocomial pathogen, Clostridioides difficile , is a spore-forming obligate anaerobe that depends on its aerotolerant spore form to transmit infections. Functional spore formation depends on the assembly of a proteinaceous layer known …
View article: Glycocluster Tetrahydroxamic Acids Exhibiting Unprecedented Inhibition of <i>Pseudomonas aeruginosa</i> Biofilms
Glycocluster Tetrahydroxamic Acids Exhibiting Unprecedented Inhibition of <i>Pseudomonas aeruginosa</i> Biofilms Open
Opportunistic Gram-negative Pseudomonas aeruginosa uses adhesins (e.g., LecA and LecB lectins, type VI pili and flagella) and iron to invade host cells with the formation of a biofilm, a thick barrier that protects bacteria from drugs and …
View article: Ligand-Mediated Biofilm Formation via Enhanced Physical Interaction between a Diguanylate Cyclase and Its Receptor
Ligand-Mediated Biofilm Formation via Enhanced Physical Interaction between a Diguanylate Cyclase and Its Receptor Open
The bacterial intracellular second messenger, cyclic dimeric GMP (c-di-GMP), regulates biofilm formation for many bacteria. The binding of c-di-GMP by the inner membrane protein LapD controls biofilm formation, and the LapD receptor is cen…
View article: Ligand-Mediated Biofilm Formation via Enhanced Physical Interaction Between a Diguanylate Cyclase and Its Receptor
Ligand-Mediated Biofilm Formation via Enhanced Physical Interaction Between a Diguanylate Cyclase and Its Receptor Open
The second messenger, cyclic dimeric GMP (c-di-GMP) regulates biofilm formation for many bacteria. The binding of c-d¡-GMP by the inner-membrane protein LapD controls biofilm formation, and the LapD receptor is central to a complex network…
View article: Iron-dependent gene expression in <i>Actinomyces oris</i>
Iron-dependent gene expression in <i>Actinomyces oris</i> Open
The growth of the sidD::Km mutant in iron-replete medium mirrored the growth of the wild-type strain grown in iron-limiting medium, suggesting that the sidD::Km mutant was compromised in iron uptake. The known iron regulator AmdR is well c…