David L. Hacker
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View article: A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response
A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response Open
The response of the adaptive immune system is augmented by multimeric presentation of a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or designed protein scaffolds, which exhibited a pot…
View article: Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies
Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies Open
In the present study, we have determined SARS-CoV-2-specific antibody responses in sera of acute and postinfection phase subjects. Our results indicate that antibody responses against viral S and N proteins were equally sensitive in the ac…
View article: Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein
Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein Open
The human membrane protein Angiotensin-converting enzyme 2 (hACE2) acts as the main receptor for host cells invasion of the new coronavirus SARS-CoV-2. The viral surface glycoprotein Spike binds to hACE2, which triggers virus entry into ce…
View article: Immune response to vaccine candidates based on different types of nanoscaffolded RBD domain of the SARS-CoV-2 spike protein
Immune response to vaccine candidates based on different types of nanoscaffolded RBD domain of the SARS-CoV-2 spike protein Open
Effective and safe vaccines against SARS-CoV-2 are highly desirable to prevent casualties and societal cost caused by Covid-19 pandemic. The receptor binding domain (RBD) of the surface-exposed spike protein of SARS-CoV-2 represents a suit…
View article: Changes in SARS-CoV-2 Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies
Changes in SARS-CoV-2 Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies Open
We have determined SARS-CoV-2-specific antibody responses in a cohort of 96 individuals with acute infection and in 578 individuals enrolled in a seroprevalence population study in Switzerland including three groups, i.e. subjects with pre…
View article: The RB596 antibody recognizes the spike S protein from SARS-CoV-2 by ELISA
The RB596 antibody recognizes the spike S protein from SARS-CoV-2 by ELISA Open
The recombinant antibody RB596 detects by ELISA the spike S protein from SARS-CoV-2.
View article: Continuous population-level monitoring of SARS-CoV-2 seroprevalence in a large metropolitan region
Continuous population-level monitoring of SARS-CoV-2 seroprevalence in a large metropolitan region Open
Effective public-health measures and vaccination campaigns against SARS-CoV-2 require granular knowledge of population-level immune responses. We developed a Tripartite Automated Blood Immunoassay (TRABI) to assess the IgG response against…
View article: AI334 and AQ806 antibodies recognize the spike S protein from SARS-CoV-2 by ELISA
AI334 and AQ806 antibodies recognize the spike S protein from SARS-CoV-2 by ELISA Open
We tested 10 recombinant antibodies directed against the spike S protein from SARS-CoV-1. Among them, antibodies AI334 and AQ806 detect by ELISA the spike S protein from SARS-CoV-2.
View article: AI334 and AQ806 antibodies recognize the spike S protein from SARS-CoV-2 by ELISA
AI334 and AQ806 antibodies recognize the spike S protein from SARS-CoV-2 by ELISA Open
We tested 10 recombinant antibodies directed against the spike S protein from SARS-CoV-1. Among them, antibodies AI334 and AQ806 detect by ELISA the spike S protein from SARS-CoV-2.
View article: Cognitive behavioural therapy plus standard care versus standard care for people with schizophrenia
Cognitive behavioural therapy plus standard care versus standard care for people with schizophrenia Open
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View article: Chromatin three-dimensional interactions mediate genetic effects on gene expression
Chromatin three-dimensional interactions mediate genetic effects on gene expression Open
Noncoding variation and gene expression Natural genetic variation outside of protein coding regions affects multiple molecular phenotypes that can differ across individuals. To examine how genomic variation affects proximal (cis) or distal…
View article: Additional file 2: of BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing
Additional file 2: of BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing Open
Table S1. List of publications referencing the Soumillon et al. 2014 study in the context of bulk RNA profiling using the SCRB-seq protocol. Table S2. List of LCL samples from the GBR population in the 1000 Genome Project. Table S3. The de…
View article: Additional file 4: of BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing
Additional file 4: of BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing Open
R script used to generate the simulated dataset with powsimR package [28]. (R 6 kb)
View article: Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes
Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes Open
Orbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50‐mL tubes with a ventilated cap…
View article: A comparison of orbitally‐shaken and stirred‐tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
A comparison of orbitally‐shaken and stirred‐tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation Open
Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred‐tank bioreactors (STRs) due to their rapid and homogeneous mixing and high o…
View article: Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Multigene expression in stable CHO cell pools generated with the piggyBac transposon system Open
Heterogenous populations of recombinant cells (cell pools) stably expressing 1–4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of t…
View article: Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines
Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines Open
Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in mammalian cells. We compared three transposons—piggyBac, Tol2, and Sleeping Beauty—for their ab…
View article: Enhanced plasmid DNA utilization in transiently transfected CHO–DG44 cells in the presence of polar solvents
Enhanced plasmid DNA utilization in transiently transfected CHO–DG44 cells in the presence of polar solvents Open
Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to red…
View article: Production of active glycosylation‐deficient γ‐secretase complex for crystallization studies
Production of active glycosylation‐deficient γ‐secretase complex for crystallization studies Open
Alzheimer's disease (AD)‐associated γ‐secretase is a ubiquitously expressed multi‐subunit protease complex embedded in the lipid bilayer of cellular compartments including endosomes and the plasma membrane. Although γ‐secretase is of cruci…