David Halfmaerten
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View article: First record of black bullhead (Ameiurus melas Lesueur, 1819) and the occurrence of hybridization with brown bullhead (A. nebulosus Rafinesque, 1820) in Belgium
First record of black bullhead (Ameiurus melas Lesueur, 1819) and the occurrence of hybridization with brown bullhead (A. nebulosus Rafinesque, 1820) in Belgium Open
sponsorship: This work was supported by Research Foundation Flanders (FWO) [No. 1S23822N and No. 1S01822N] . Publication of this study was supported by the European Union's Horizon Europe HORIZON-CL6-2024-BIODIV-01 project "GuardIAS-Guardi…
View article: Environmental DNA metabarcoding reflects spatiotemporal fish community shifts in the Scheldt estuary
Environmental DNA metabarcoding reflects spatiotemporal fish community shifts in the Scheldt estuary Open
Estuarine ecosystems face increasing anthropogenic pressures, necessitating effective monitoring methods to mitigate their impacts on the biodiversity they harbour. The use of environmental DNA (eDNA) based detection methods is increasingl…
View article: 12S metabarcoding reference data from the Research Institute for Nature and Forest (INBO)
12S metabarcoding reference data from the Research Institute for Nature and Forest (INBO) Open
This dataset contains metabarcoding reference data with 12S sequences from fish and other vertebrates for Teleo and Riaz primers suited for use with the OBITools package. Files- all_seqs_INBO_riaz_amplified.fasta: reference data for Riaz m…
View article: 12S metabarcoding reference data from the Research Institute for Nature and Forest (INBO)
12S metabarcoding reference data from the Research Institute for Nature and Forest (INBO) Open
This dataset contains metabarcoding reference data with 12S sequences from fish and other vertebrates for Teleo and Riaz primers suited for use with the OBITools package. Files- all_seqs_INBO_riaz_amplified.fasta: reference data for Riaz m…
View article: Combining multiple markers significantly increases the sensitivity and precision of <scp>eDNA</scp>‐based single‐species analyses
Combining multiple markers significantly increases the sensitivity and precision of <span>eDNA</span>‐based single‐species analyses Open
The analysis of environmental DNA (eDNA) is becoming integrated as an established biomonitoring tool, often characterized by detection limits exceeding those of conventional counterparts. However, further improving the sensitivity of these…
View article: Using environmental DNA metabarcoding to monitor fish communities in small rivers and large brooks: Insights on the spatial scale of information
Using environmental DNA metabarcoding to monitor fish communities in small rivers and large brooks: Insights on the spatial scale of information Open
Monitoring fish communities is central to the evaluation of ecological health of rivers. Both presence/absence of fish species and their relative quantity in local fish assemblages are crucial parameters to measure. Fish communities in lot…
View article: LifeDNAquatic: A priori settings and refined pipelines of eDNA metabarcoding to generate "big data" for solid fish, mussel and amphibian SDMs
LifeDNAquatic: A priori settings and refined pipelines of eDNA metabarcoding to generate "big data" for solid fish, mussel and amphibian SDMs Open
Sound environmental management decisions - in accordance with the EU WFD for aquatic ecosystems – mainly depend on reliable species presence- and distribution- data. Here we present a workflow from sampling strategies to results and decisi…
View article: The use of multiple markers and internal positive controls significantly improves species eDNA detection rates and data reliability
The use of multiple markers and internal positive controls significantly improves species eDNA detection rates and data reliability Open
In recent years, environmental DNA analyses became increasingly integrated to detect and monitor the presence and abundance of rare organisms, especially in inaccessible aquatic habitats. Although it is generally proven that detection prob…
View article: Effects of preservation strategies on environmental DNA detection and quantification using ddPCR
Effects of preservation strategies on environmental DNA detection and quantification using ddPCR Open
Molecular‐based monitoring relying on environmental DNA (eDNA) detection became routinely used around the world in the last few years, especially in aquatic environments. The large potential and increasing applications of this technique ca…
View article: The invasive parthenogenetic marbled crayfish Procambarus virginalis Lyko, 2017 gets foothold in Belgium
The invasive parthenogenetic marbled crayfish Procambarus virginalis Lyko, 2017 gets foothold in Belgium Open
In 2020, four populations of the marbled crayfish Procambarus virginalis, which is included on the list of Invasive Alien Species of Union concern, were discovered in northern Belgium. These records represent the first established populati…
View article: Accurate Detection and Quantification of Seasonal Abundance of American Bullfrog (Lithobates Catesbeianus) Using ddPCR eDNA Assays
Accurate Detection and Quantification of Seasonal Abundance of American Bullfrog (Lithobates Catesbeianus) Using ddPCR eDNA Assays Open
The invasive American bullfrog ( Lithobates catesbeianus ) imperils freshwater biodiversity worldwide. Effective management includes rapid detection and response to incipient invasions, as established populations are extremely difficult to…
View article: Monitoring of spatiotemporal occupancy patterns of fish and amphibian species in a lentic aquatic system using environmental DNA
Monitoring of spatiotemporal occupancy patterns of fish and amphibian species in a lentic aquatic system using environmental DNA Open
To effectively monitor, manage and protect aquatic species and understand their interactions, knowledge of their spatiotemporal distribution is needed. In this study, we used a fine‐scale spatiotemporal water sampling design, followed by e…
View article: 12S metabarcoding reference data from the Research Institute for Nature and Forest (INBO)
12S metabarcoding reference data from the Research Institute for Nature and Forest (INBO) Open
This dataset contains metabarcoding reference data with 12S sequences from fish and other vertebrates for Teleo and Riaz primers suited for use with the OBITools package. Files - all_seqs_INBO_riaz_amplified.fasta: reference data for Riaz …
View article: On the presence of the invasive planarian Obama nungara (Carbayo, Álvarez-Presas, Jones & Riutort, 2016) (Platyhelminthes: Geoplanidae) in an urban area in Belgium
On the presence of the invasive planarian Obama nungara (Carbayo, Álvarez-Presas, Jones & Riutort, 2016) (Platyhelminthes: Geoplanidae) in an urban area in Belgium Open
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View article: Comparison of capture and storage methods for aqueous macrobial <scp>eDNA</scp> using an optimized extraction protocol: advantage of enclosed filter
Comparison of capture and storage methods for aqueous macrobial <span>eDNA</span> using an optimized extraction protocol: advantage of enclosed filter Open
Summary Aqueous environmental DNA ( eDNA ) is an emerging efficient non‐invasive tool for species inventory studies. To maximize performance of downstream quantitative PCR (qPCR) and next‐generation sequencing ( NGS ) applications, quality…