David I. Stuart
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View article: Electron fourier ptychography for phase reconstruction
Electron fourier ptychography for phase reconstruction Open
View article: Redundant Late Domain Functions of Tandem VP2 YPX3L Motifs in Nonlytic Cellular Egress of Quasi-enveloped Hepatitis A Virus
Redundant Late Domain Functions of Tandem VP2 YPX3L Motifs in Nonlytic Cellular Egress of Quasi-enveloped Hepatitis A Virus Open
The quasi-envelopment of hepatitis A virus (HAV) capsids in exosome-like virions (eHAV) is an important but incompletely understood aspect of the hepatovirus life cycle. This process is driven by recruitment of newly assembled capsids to e…
View article: Assessing the Possibility of Recurrent Diabetic Foot Ulcer Prevention via Remote Patient Monitoring: A Feasibility Study
Assessing the Possibility of Recurrent Diabetic Foot Ulcer Prevention via Remote Patient Monitoring: A Feasibility Study Open
Aims Our main aims in this study of diabetic patients at risk of foot ulcers were to evaluate: (a) adherence to the use of an at‐home thermal and visual digital foot scanner, (b) the feasibility of utilising thermovisual scan data to perfo…
View article: Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development
Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development Open
Influenza virus neuraminidase (NA) is a crucial target for protective antibodies, yet the development of recombinant NA protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: Author response: Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development
Author response: Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development Open
View article: Capsid Restructuring Activates Semi-Conservative dsRNA Transcription in Cystovirus ɸ6
Capsid Restructuring Activates Semi-Conservative dsRNA Transcription in Cystovirus ɸ6 Open
Summary Double-stranded (ds)RNA viruses replicate and transcribe their genome within a proteinaceous viral capsid to evade host cell defenses. While Reovirale s members use conservative transcription, most dsRNA viruses, including cystovir…
View article: Author Correction: An RBD-Fc mucosal vaccine provides variant-proof protection against SARS-CoV-2 in mice and hamsters
Author Correction: An RBD-Fc mucosal vaccine provides variant-proof protection against SARS-CoV-2 in mice and hamsters Open
View article: An RBD-Fc mucosal vaccine provides variant-proof protection against SARS-CoV-2 in mice and hamsters
An RBD-Fc mucosal vaccine provides variant-proof protection against SARS-CoV-2 in mice and hamsters Open
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza virus neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approac…
View article: Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
View article: A Teotihuacan altar at Tikal, Guatemala: central Mexican ritual and elite interaction in the Maya Lowlands
A Teotihuacan altar at Tikal, Guatemala: central Mexican ritual and elite interaction in the Maya Lowlands Open
The nature and extent of interactions between the distant regions and cultures of Mesoamerica remain open to much debate. Close economic and political ties developed between Teotihuacan and the lowland Maya during the Early Classic period …
View article: Production of an immunogenic trivalent poliovirus virus-like particle vaccine candidate in yeast using controlled fermentation
Production of an immunogenic trivalent poliovirus virus-like particle vaccine candidate in yeast using controlled fermentation Open
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
View article: Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development
Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development Open
Influenza virus neuraminidase (NA) is a crucial target for protective antibodies, yet the development of recombinant NA protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: Electron Fourier ptychography for phase reconstruction
Electron Fourier ptychography for phase reconstruction Open
Phase reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive materials. W…
View article: <i>TomoCPT</i>: a generalizable model for 3D particle detection and localization in cryo-electron tomograms
<i>TomoCPT</i>: a generalizable model for 3D particle detection and localization in cryo-electron tomograms Open
Cryo-electron tomography is a rapidly developing field for studying macromolecular complexes in their native environments and has the potential to revolutionize our understanding of protein function. However, fast and accurate identificati…
View article: Recombinant expression systems for production of stabilised virus-like particles as next-generation polio vaccines
Recombinant expression systems for production of stabilised virus-like particles as next-generation polio vaccines Open
Polioviruses have caused crippling disease in humans for centuries, prior to the successful development of vaccines in the mid-1900’s, which dramatically reduced disease prevalence. Continued use of these vaccines, however, threatens ultim…
View article: 25 - Understanding pelvic floor dysfunction post-pelvic fracture.
25 - Understanding pelvic floor dysfunction post-pelvic fracture. Open
View article: From Voxels to Viruses: Using Deep Learning and Crowdsourcing to Understand a Virus Factory
From Voxels to Viruses: Using Deep Learning and Crowdsourcing to Understand a Virus Factory Open
Many bioimaging research projects require objects of interest to be identified, located, and then traced to allow quantitative measurement. Depending on the complexity of the system and imaging, instance segmentation is often done manually…
View article: Upscaling production of immunogenic poliovirus virus-like particles in<i>Pichia Pastoris</i>by controlled fermentation
Upscaling production of immunogenic poliovirus virus-like particles in<i>Pichia Pastoris</i>by controlled fermentation Open
The success of the poliovirus (PV) vaccines has enabled the near-eradication of wild PV, however, their continued use post-eradication poses a significant threat to maintaining a polio-free world. Recombinant virus-like particles (VLPs) th…
View article: A broadly reactive ultralong bovine antibody that can determine the integrity of foot-and-mouth disease virus capsids
A broadly reactive ultralong bovine antibody that can determine the integrity of foot-and-mouth disease virus capsids Open
Foot-and-mouth disease vaccination using inactivated virus is suboptimal, as the icosahedral viral capsids often disassemble into antigenically distinct pentameric units during long-term storage, or exposure to elevated temperature or lowe…
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza virus neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approac…
View article: Structure of the RNA-dependent RNA polymerase P2 from the cystovirus φ8
Structure of the RNA-dependent RNA polymerase P2 from the cystovirus φ8 Open
The replication of RNA viruses relies on the activity of RNA-dependent RNA polymerases (RdRps). Despite large variations in their genomic sequences, viral RdRps share a common architecture generally known as a closed right hand. The P2 pol…
View article: Oligomerization-driven avidity correlates with SARS-CoV-2 cellular binding and inhibition
Oligomerization-driven avidity correlates with SARS-CoV-2 cellular binding and inhibition Open
Cellular processes are controlled by the thermodynamics of the underlying biomolecular interactions. Frequently, structural investigations use one monomeric binding partner, while ensemble measurements of binding affinities generally yield…
View article: Concerted deletions eliminate a neutralizing supersite in SARS-CoV-2 BA.2.87.1 spike
Concerted deletions eliminate a neutralizing supersite in SARS-CoV-2 BA.2.87.1 spike Open
BA.2.87.1 represents a major shift in the BA.2 lineage of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is unusual in having two lengthy deletions of polypeptide in the spike (S) protein, one of which removes a beta-stra…
View article: The effect of pH on the structure of Bluetongue virus VP5
The effect of pH on the structure of Bluetongue virus VP5 Open
The unenveloped Bluetongue virus capsid comprises several structural layers, the inner two comprising a core, which assembles before addition of the outer proteins, VP2 and VP5. Two symmetric trimers of VP5 fit like pegs into two distinct …
View article: Immunogenicity of Abdala COVID-19 vaccine in Vietnamese people after primary and booster vaccinations: A prospective observational study in Vietnam
Immunogenicity of Abdala COVID-19 vaccine in Vietnamese people after primary and booster vaccinations: A prospective observational study in Vietnam Open
Abdala COVID-19 vaccine is immunogenic in Vietnamese people. Enhanced antibody response after a booster dose could cross-neutralize 14 SARS-CoV-2 variants and SARS-CoV-1. Our results have added to the growing body of knowledge about the co…
View article: Amino acid and viral binding by the high-affinity Cationic Amino acidTransporter 1 (CAT1) from Mus musculus
Amino acid and viral binding by the high-affinity Cationic Amino acidTransporter 1 (CAT1) from Mus musculus Open
Arginine, lysine, and ornithine are critical to protein structure and function, the urea cycle, and intracellular signaling. These cationic amino acids are imported by several membrane transporters, most notably the Cationic Amino acid Tra…
View article: Anti-SARS-CoV-2 antibody dynamics after primary vaccination with two-dose inactivated whole-virus vaccine, heterologous mRNA-1273 vaccine booster, and Omicron breakthrough infection in Indonesian health care workers
Anti-SARS-CoV-2 antibody dynamics after primary vaccination with two-dose inactivated whole-virus vaccine, heterologous mRNA-1273 vaccine booster, and Omicron breakthrough infection in Indonesian health care workers Open