David Kopecký
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View article: A genus-wide pangenome of Hordeum
A genus-wide pangenome of Hordeum Open
The genus Hordeum, which includes cultivated barley and its wild relatives, represents a diverse group of grasses with agricultural, ecological, and evolutionary importance. Here, we present a genus-wide pangenome based on 25 chromosome-sc…
View article: Restoring cytonuclear harmony: Distinct strategies in Arabidopsis auto‐ and allopolyploids
Restoring cytonuclear harmony: Distinct strategies in Arabidopsis auto‐ and allopolyploids Open
SUMMARY Plants rely on tight coordination between nuclear, mitochondrial, and chloroplast genomes to form essential multi‐enzyme cytonuclear complexes. Whole‐genome duplication (WGD) doubles the nuclear genome, potentially disrupting cyton…
View article: Proportion of parental genomes in hybrids <i>Allium cepa × A. roylei</i> determines which one becomes dominant
Proportion of parental genomes in hybrids <i>Allium cepa × A. roylei</i> determines which one becomes dominant Open
Interspecific hybridization leads to complex interactions between the parental genomes, often in the form of genome dominance, where one genome prevails over the other. This phenomenon has been attributed to differential chromosome behavio…
View article: Cytonuclear interplay in auto‐ and allopolyploids: a multifaceted perspective from the <scp><i>Festuca‐Lolium</i></scp> complex
Cytonuclear interplay in auto‐ and allopolyploids: a multifaceted perspective from the <span><i>Festuca‐Lolium</i></span> complex Open
SUMMARY Restoring cytonuclear stoichiometry is necessary after whole‐genome duplication (WGD) and interspecific/intergeneric hybridization in plants. We investigated this phenomenon in auto‐ and allopolyploids of the Festuca‐Lolium complex…
View article: Morphological, flow cytometric and cytogenetic evidence of a hybrid swarm between Elymus hispidus and E. repens in the Bílé Karpaty Mts, Czech Republic
Morphological, flow cytometric and cytogenetic evidence of a hybrid swarm between Elymus hispidus and E. repens in the Bílé Karpaty Mts, Czech Republic Open
View article: Uniparental expression of ribosomal RNA in ×Festulolium grasses: a link between the genome and nucleolar dominance
Uniparental expression of ribosomal RNA in ×Festulolium grasses: a link between the genome and nucleolar dominance Open
Genome or genomic dominance (GD) is a phenomenon observed in hybrids when one parental genome becomes dominant over the other. It is manifested by the replacement of chromatin of the submissive genome by that of the dominant genome and by …
View article: Telomerase <scp>RNA</scp> gene paralogs in plants – the usual pathway to unusual telomeres
Telomerase <span>RNA</span> gene paralogs in plants – the usual pathway to unusual telomeres Open
Summary Telomerase, telomeric DNA and associated proteins together represent a complex, finely tuned and functionally conserved mechanism that ensures genome integrity by protecting and maintaining chromosome ends. Changes in its component…
View article: Both male and female meiosis contribute to non‐Mendelian inheritance of parental chromosomes in interspecific plant hybrids (<i>Lolium</i> × <i>Festuca</i>)
Both male and female meiosis contribute to non‐Mendelian inheritance of parental chromosomes in interspecific plant hybrids (<i>Lolium</i> × <i>Festuca</i>) Open
Summary Some interspecific plant hybrids show unequal transmission of chromosomes from parental genomes to the successive generations. It has been suggested that this is due to a differential behavior of parental chromosomes during meiosis…
View article: Interspecific hybridization and plant breeding: From historical retrospective through work of Mendel to current crops
Interspecific hybridization and plant breeding: From historical retrospective through work of Mendel to current crops Open
There is a relatively long history of plant hybridization traced back to ancient time, both from theoretical as well as practical perspectives. At first considered as an evolutionary dead-end, it was soon recognized to have important role …
View article: Genome Dominance in Allium Hybrids (A. cepa × A. roylei)
Genome Dominance in Allium Hybrids (A. cepa × A. roylei) Open
Genome dominance is a phenomenon in wide hybrids when one of the parental genomes becomes “dominant,” while the other genome turns to be “submissive.” This dominance may express itself in several ways including homoeologous gene expression…
View article: Reciprocal allopolyploid grasses (<i>Festuca</i> × <i>Lolium</i>) display stable patterns of genome dominance
Reciprocal allopolyploid grasses (<i>Festuca</i> × <i>Lolium</i>) display stable patterns of genome dominance Open
Summary Allopolyploidization entailing the merger of two distinct genomes in a single hybrid organism, is an important process in plant evolution and a valuable tool in breeding programs. Newly established hybrids often experience massive …
View article: Horizontally Acquired nrDNAs Persist in Low Amounts in Host Hordeum Genomes and Evolve Independently of Native nrDNA
Horizontally Acquired nrDNAs Persist in Low Amounts in Host Hordeum Genomes and Evolve Independently of Native nrDNA Open
Nuclear ribosomal DNA (nrDNA) has displayed extraordinary dynamics during the evolution of plant species. However, the patterns and evolutionary significance of nrDNA array expansion or contraction are still relatively unknown. Moreover, o…
View article: Future agroclimatic conditions and implications for European grasslands
Future agroclimatic conditions and implications for European grasslands Open
Grasslands play a significant role in livestock fodder production and thus, contribute to food security worldwide while providing numerous additional ecosystem services. However, how agroclimatic conditions and adverse weather events relev…
View article: A <i>Panicum</i>‐derived chromosomal segment captured by <i>Hordeum</i> a few million years ago preserves a set of stress‐related genes
A <i>Panicum</i>‐derived chromosomal segment captured by <i>Hordeum</i> a few million years ago preserves a set of stress‐related genes Open
Summary Intra‐specific variability is a cornerstone of evolutionary success of species. Acquiring genetic material from distant sources is an important adaptive mechanism in bacteria, but it can also play a role in eukaryotes. In this pape…
View article: Spontaneous natural formation of interspecific hybrids within the Festuca-Lolium complex
Spontaneous natural formation of interspecific hybrids within the Festuca-Lolium complex Open
Interspecific and intergeneric hybridization within the Festuca-Lolium complex is frequently used in forage plant breeding. However, little is known about the natural occurrence and competitiveness of such hybrids. We collected naturally f…
View article: Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (<i>Lolium multiflorum×Festuca pratensis</i>and<i>Allium cepa×A. roylei</i>)
Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (<i>Lolium multiflorum×Festuca pratensis</i>and<i>Allium cepa×A. roylei</i>) Open
Crossing over, in addition to its strictly genetic role, also performs a critical mechanical function, by bonding homologues in meiosis. Hence, it is responsible for an orderly reduction of the chromosome number. As such, it is strictly co…
View article: Cytogenetic insights into Festulolium
Cytogenetic insights into Festulolium Open
Climate change calls for new methods and plant materials to breed crops adapted to new environmental conditions. Sustainable forage and amenity grass production during periods of severe drought and heat waves during summer, and unequal dis…
View article: Drought tolerance and regrowth capacity revealed in the Festuca-Lolium complex
Drought tolerance and regrowth capacity revealed in the Festuca-Lolium complex Open
The climate change appears to have accelerated in recent years, and more changes are envisaged in the near future. With this in mind, breeders should consider the choices of materials to be used in breeding for the future to potentially mi…
View article: Triploid forage grass hybrids Festuca apennina × F. pratensis display extraordinary heterosis for yield characteristics
Triploid forage grass hybrids Festuca apennina × F. pratensis display extraordinary heterosis for yield characteristics Open
Hybrid vigor (or heterosis) is the over-performance of a hybrid over its parents in a specific trait or a set of traits. As such, hybrid breeding serves as a tool to efficiently trigger gains in breeding programs. Moreover, hybrids of gene…
View article: Chromosome Pairing in Polyploid Grasses
Chromosome Pairing in Polyploid Grasses Open
Polyploids are species in which three or more sets of chromosomes coexist. Polyploidy frequently occurs in plants and plays a major role in their evolution. Based on their origin, polyploid species can be divided into two groups: autopolyp…
View article: Comparative analyses of DNA repeats and identification of a novel Fesreba centromeric element in fescues and ryegrasses
Comparative analyses of DNA repeats and identification of a novel Fesreba centromeric element in fescues and ryegrasses Open
View article: Comparative analysis of DNA repeats and identification of novel Fesreba centromeric element in fescues and ryegrasses
Comparative analysis of DNA repeats and identification of novel Fesreba centromeric element in fescues and ryegrasses Open
Background Cultivated grasses are an important source of food for domestic animals worldwide. Better knowledge of their genomes can speed up the development of new cultivars with better quality and resistance to biotic and abiotic stresses…
View article: Competition of Parental Genomes in Plant Hybrids
Competition of Parental Genomes in Plant Hybrids Open
Interspecific hybridization represents one of the main mechanisms of plant speciation. Merging of two genomes from different subspecies, species, or even genera is frequently accompanied by whole-genome duplication (WGD). Besides its evolu…
View article: Comparative analysis of DNA repeats and identification of a Fesreba centromeric element in fescues and ryegrasses
Comparative analysis of DNA repeats and identification of a Fesreba centromeric element in fescues and ryegrasses Open
Background Cultivated grasses are an important source of food for domestic animals worldwide. Better knowledge of their genomes can speed up development of cultivars with better quality and resistance to biotic and abiotic stresses. The mo…
View article: Extraordinary Heterosis Found In A Forage Grass Hybrid, Festuca apennina × F. pratensis
Extraordinary Heterosis Found In A Forage Grass Hybrid, Festuca apennina × F. pratensis Open
Background: Heterosis (or hybrid vigor) is the over-performance of a hybrid over its parents in a specific trait or a set of traits. As such, hybrid breeding serves as a tool to efficiently trigger gains in breeding programs. Moreover, hyb…
View article: Nuclear Disposition of Alien Chromosome Introgressions into Wheat and Rye Using 3D-FISH
Nuclear Disposition of Alien Chromosome Introgressions into Wheat and Rye Using 3D-FISH Open
During interphase, the chromosomes of eukaryotes decondense and they occupy distinct regions of the nucleus, called chromosome domains or chromosome territories (CTs). In plants, the Rabl’s configuration, with telomeres at one pole of nucl…
View article: Lokalizace a pokročilé řízení autonomního modelu vozidla
Lokalizace a pokročilé řízení autonomního modelu vozidla Open
Tato práce se zabývá mapováním a lokalizací na závodní dráze F1/10 autonomous driving competition pomocí zmenšeného modelu vozidla. Lokalizace využívá Monte Carlo metod pro zpracování dat LiDARu a odhad pozice vozidla. Implementovaný systé…
View article: Selective Elimination of Parental Chromatin from Introgression Cultivars of xFestulolium (Festuca × Lolium)
Selective Elimination of Parental Chromatin from Introgression Cultivars of xFestulolium (Festuca × Lolium) Open
Alien chromosome introgressions can be used to introduce beneficial traits from one species into another. However, exploitation of the introgressions in breeding requires proper transmission of introgressed segments to consecutive generati…
View article: Accessing a Russian Wheat Aphid Resistance Gene in Bread Wheat by Long‐Read Technologies
Accessing a Russian Wheat Aphid Resistance Gene in Bread Wheat by Long‐Read Technologies Open
Russian wheat aphid (RWA) ( Diuraphis noxia Kurdjumov) is a serious invasive pest of small‐grain cereals and many grass species. An efficient strategy to defy aphid attacks is to identify sources of natural resistance and transfer resistan…
View article: Instability of Alien Chromosome Introgressions in Wheat Associated with Improper Positioning in the Nucleus
Instability of Alien Chromosome Introgressions in Wheat Associated with Improper Positioning in the Nucleus Open
Alien introgressions introduce beneficial alleles into existing crops and hence, are widely used in plant breeding. Generally, introgressed alien chromosomes show reduced meiotic pairing relative to the host genome, and may be eliminated o…