David L. Prole
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View article: Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology
Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology Open
G protein-coupled receptor (GPCR) signalling pathways underlie numerous physiological processes, are implicated in many diseases and are major targets for therapeutics. There are more than 800 GPCRs, which together transduce a vast array o…
View article: Golgi anti-apoptotic proteins are evolutionarily conserved ion channels that regulate cell death in plants
Golgi anti-apoptotic proteins are evolutionarily conserved ion channels that regulate cell death in plants Open
Programmed cell death regulates developmental and stress responses in eukaryotes. Golgi anti-apoptotic proteins (GAAPs) are evolutionarily conserved cell death regulators. Human and viral GAAPs inhibit apoptosis and modulate intracellular …
View article: Structure and Function of IP<sub>3</sub>Receptors
Structure and Function of IP<sub>3</sub>Receptors Open
Inositol 1,4,5-trisphosphate receptors (IP3Rs), by releasing Ca2+ from the endoplasmic reticulum (ER) of animal cells, allow Ca2+ to be redistributed from the ER to the cytosol or other organelles, and they initiate store-operated Ca2+ ent…
View article: A genetically-encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling
A genetically-encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling Open
Background Intrabodies enable targeting of proteins in live cells, but it remains a huge task to generate specific intrabodies against the thousands of proteins in a proteome. We leverage the widespread availability of fluorescently labell…
View article: Exploration of inositol 1,4,5-trisphosphate (IP<sub>3</sub>) regulated dynamics of N-terminal domain of IP<sub>3</sub> receptor reveals early phase molecular events during receptor activation
Exploration of inositol 1,4,5-trisphosphate (IP<sub>3</sub>) regulated dynamics of N-terminal domain of IP<sub>3</sub> receptor reveals early phase molecular events during receptor activation Open
Inositol 1, 4, 5-trisphosphate (IP 3 ) binding at the N-terminus (NT) of IP 3 receptor (IP 3 R) allosterically triggers the opening of a Ca 2+ -conducting pore located ~ 100 Å away from the IP 3 -binding core (IBC). However, the precise me…
View article: Immobile IP3 Receptor Clusters: Building Blocks For IP3-Evoked Ca2+ Signals
Immobile IP3 Receptor Clusters: Building Blocks For IP3-Evoked Ca2+ Signals Open
Co-regulation of IP3 receptors (IP3Rs) by IP3 and cytosolic Ca2+ allows them to mediate regenerative signals, amongst which are Ca2+ puffs. These reflect the near-simultaneous opening of a few IP3Rs within a small cluster. A long-standing …
View article: Inositol 1,4,5‐trisphosphate receptors and their protein partners as signalling hubs
Inositol 1,4,5‐trisphosphate receptors and their protein partners as signalling hubs Open
Inositol 1,4,5‐trisphosphate receptors (IP 3 Rs) are expressed in nearly all animal cells, where they mediate the release of Ca 2+ from intracellular stores. The complex spatial and temporal organization of the ensuing intracellular Ca 2+ …