David Lalaouna
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View article: Coordination of the Fe-S cluster biogenesis network by the sRNA RyhB in <i>E. coli</i>
Coordination of the Fe-S cluster biogenesis network by the sRNA RyhB in <i>E. coli</i> Open
Iron (Fe) plays critical roles as enzyme cofactor involved in key biological processes but can also lead to toxicity by catalysing the formation of highly damaging reactive oxygen species. To stabilize Fe and perform catalysis, most organi…
View article: The iron-regulated small regulatory RNA IsrR modulates expression of genes utilized for dioxygen metabolism and heme synthesis in <i>Staphylococcus aureus</i>
The iron-regulated small regulatory RNA IsrR modulates expression of genes utilized for dioxygen metabolism and heme synthesis in <i>Staphylococcus aureus</i> Open
Bacterial regulatory RNAs (sRNAs) are commonly short non-coding RNAs that function as pleiotropic regulators by post-transcriptionally impacting mRNA stability and/or translation. They play significant roles in bacterial physiology and are…
View article: A Zur-dependent regulatory RNA involved in maintaining zinc homeostasis in <i>Staphylococcus aureus</i>
A Zur-dependent regulatory RNA involved in maintaining zinc homeostasis in <i>Staphylococcus aureus</i> Open
Small regulatory RNAs (sRNAs) are key drivers of bacterial adaptation to environmental fluctuations, including iron and manganese restriction imposed by the host. This study explored the repertoire of sRNAs produced by the human pathogen S…
View article: A manganese-sparing response balances competing cellular demands to enable <i>Staphylococcus aureus</i> infection
A manganese-sparing response balances competing cellular demands to enable <i>Staphylococcus aureus</i> infection Open
Responding to stress is critical to the survival of life, especially for microbes that have a limited ability to manipulate their environment. During infection, Staphylococcus aureus and other invaders must overcome both the host-imposed a…
View article: What makes a small RNA work?
What makes a small RNA work? Open
Bacterial small RNAs (sRNAs) are key regulators of gene expression, interacting with target messenger RNAs (mRNAs) through imperfect base pairing. Unlike other non-coding RNAs such as microRNAs and PIWI-interacting RNAs, bacterial sRNAs ex…
View article: The <i>Staphylococcus aureus</i> non-coding RNA IsrR regulates TCA cycle activity and virulence
The <i>Staphylococcus aureus</i> non-coding RNA IsrR regulates TCA cycle activity and virulence Open
Staphylococcus aureus has evolved mechanisms to cope with low iron (Fe) availability in host tissues. Staphylococcus aureus uses the ferric uptake transcriptional regulator (Fur) to sense titers of cytosolic Fe. Upon Fe depletion, apo-Fur …
View article: Coordination of cell division and chromosome segregation by iron and a sRNA in Escherichia coli
Coordination of cell division and chromosome segregation by iron and a sRNA in Escherichia coli Open
Iron is a vital metal ion frequently present as a cofactor in metabolic enzymes involved in central carbon metabolism, respiratory chain, and DNA synthesis. Notably, iron starvation was previously shown to inhibit cell division, although t…
View article: An unprecedented small RNA-riboswitch interaction controls expression of a bifunctional pump that is essential for<i>Staphylococcus aureus</i>infection
An unprecedented small RNA-riboswitch interaction controls expression of a bifunctional pump that is essential for<i>Staphylococcus aureus</i>infection Open
SUMMARY Maintaining manganese and iron homeostasis is critical for the human pathogen Staphylococcus aureus . To counteract metal-based host defense strategies (e.g., nutritional immunity, metal poisoning), S. aureus uses a combination of …
View article: The<i>Staphylococcus aureus</i>small non-coding RNA IsrR regulates TCA cycle activity and virulence
The<i>Staphylococcus aureus</i>small non-coding RNA IsrR regulates TCA cycle activity and virulence Open
Staphylococcus aureus has evolved mechanisms to cope with low iron (Fe) availability in host tissues. S. aureus uses the ferric uptake transcriptional regulator (Fur) to sense titers of cytosolic Fe. Upon Fe depletion, apo-Fur relieves tra…
View article: The 3′ UTR of vigR is required for virulence in Staphylococcus aureus and has expanded through STAR sequence repeat insertions
The 3′ UTR of vigR is required for virulence in Staphylococcus aureus and has expanded through STAR sequence repeat insertions Open
View article: The 3’ UTR of<i>vigR</i>is required for virulence in<i>Staphylococcus aureus</i>and has expanded through STAR sequence repeat insertions
The 3’ UTR of<i>vigR</i>is required for virulence in<i>Staphylococcus aureus</i>and has expanded through STAR sequence repeat insertions Open
Staphylococcus aureus is an adaptable human pathogen causing life-threatening endocarditis and bacteraemia. Methicillin-resistant S. aureus (MRSA) is alarmingly common, and treatment is confined to last-line antibiotics. Vancomycin is the …
View article: Hfq protein and GcvB small RNA tailoring of <i>oppA</i> target mRNA to levels allowing translation activation by MicF small RNA in <i>Escherichia coli</i>
Hfq protein and GcvB small RNA tailoring of <i>oppA</i> target mRNA to levels allowing translation activation by MicF small RNA in <i>Escherichia coli</i> Open
Traffic of molecules across the bacterial membrane mainly relies on porins and transporters, whose expression must adapt to environmental conditions. To ensure bacterial fitness, synthesis and assembly of functional porins and transporters…
View article: Hfq protein and GcvB small RNA tailoring of <i>oppA</i> target mRNA to levels allowing translation activation by MicF small RNA in <i>Escherichia coli</i>
Hfq protein and GcvB small RNA tailoring of <i>oppA</i> target mRNA to levels allowing translation activation by MicF small RNA in <i>Escherichia coli</i> Open
Traffic of molecules across the bacterial membrane mainly relies on porins and transporters, whose expression must adapt to environmental conditions. To ensure bacterial fitness, synthesis and assembly of functional porins and transporters…
View article: A tRNA-derived fragment present in E. coli OMVs regulates host cell gene expression and proliferation
A tRNA-derived fragment present in E. coli OMVs regulates host cell gene expression and proliferation Open
RNA-sequencing has led to a spectacular increase in the repertoire of bacterial sRNAs and improved our understanding of their biological functions. Bacterial sRNAs have also been found in outer membrane vesicles (OMVs), raising questions a…
View article: Battle for Metals: Regulatory RNAs at the Front Line
Battle for Metals: Regulatory RNAs at the Front Line Open
Metal such as iron, zinc, manganese, and nickel are essential elements for bacteria. These nutrients are required in crucial structural and catalytic roles in biological processes, including precursor biosynthesis, DNA replication, transcr…
View article: RNA Sequencing Unveils Very Small RNAs With Potential Regulatory Functions in Bacteria
RNA Sequencing Unveils Very Small RNAs With Potential Regulatory Functions in Bacteria Open
RNA sequencing (RNA-seq) is the gold standard for the discovery of small non-coding RNAs. Following a long-standing approach, reads shorter than 16 nucleotides (nt) are removed from the small RNA sequencing libraries or datasets. The seren…
View article: Phytobeneficial traits of rhizobacteria under the control of multiple molecular dialogues
Phytobeneficial traits of rhizobacteria under the control of multiple molecular dialogues Open
Summary Pseudomonads play crucial roles in plant growth promotion and control of plant diseases. However, under natural conditions, other microorganisms competing for the same nutrient resources in the rhizosphere may exert negative contro…
View article: The noncoding RNA CcnA modulates the master cell cycle regulators CtrA and GcrA in Caulobacter crescentus
The noncoding RNA CcnA modulates the master cell cycle regulators CtrA and GcrA in Caulobacter crescentus Open
Bacteria are powerful models for understanding how cells divide and accomplish global regulatory programs. In Caulobacter crescentus , a cascade of essential master regulators supervises the correct and sequential activation of DNA replica…
View article: Binding of the RNA Chaperone Hfq on Target mRNAs Promotes the Small RNA RyhB-Induced Degradation in Escherichia coli
Binding of the RNA Chaperone Hfq on Target mRNAs Promotes the Small RNA RyhB-Induced Degradation in Escherichia coli Open
Many RNA-RNA interactions depend on molecular chaperones to form and remain stable in living cells. A prime example is the RNA chaperone Hfq, which is a critical effector involved in regulatory interactions between small RNAs (sRNAs) and c…
View article: Burning the Candle at Both Ends: Have Exoribonucleases Driven Divergence of Regulatory RNA Mechanisms in Bacteria?
Burning the Candle at Both Ends: Have Exoribonucleases Driven Divergence of Regulatory RNA Mechanisms in Bacteria? Open
Regulatory RNAs have emerged as ubiquitous gene regulators in all bacterial species studied to date. The combination of sequence-specific RNA interactions and malleable RNA structure has allowed regulatory RNA to adopt different mechanisms…
View article: Assembling the Current Pieces: The Puzzle of RNA-Mediated Regulation in Staphylococcus aureus
Assembling the Current Pieces: The Puzzle of RNA-Mediated Regulation in Staphylococcus aureus Open
The success of the major opportunistic human Staphylococcus aureus relies on the production of numerous virulence factors, which allow rapid colonization and dissemination in any tissues. Indeed, regulation of its virulence is multifactori…
View article: Evolutionary history expands the range of signaling interactions in hybrid multikinase networks
Evolutionary history expands the range of signaling interactions in hybrid multikinase networks Open
View article: MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria Open
Although small regulatory RNAs (sRNAs) are widespread among the bacterial domain of life, the functions of many of them remain poorly characterized notably due to the difficulty of identifying their mRNA targets. Here, we described a modif…
View article: Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival of Pseudomonas brassicacerum in Nutrient-Poor Environments
Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival of Pseudomonas brassicacerum in Nutrient-Poor Environments Open
In the beneficial plant root-associated Pseudomonas brassicacearum strain NFM421, the GacS/GacA two-component system positively controls biofilm formation and the production of secondary metabolites through the synthesis of rsmX, rsmY and …
View article: Manganese: the battle of the two armies
Manganese: the battle of the two armies Open
View article: Manganese: the battle of the two armies
Manganese: the battle of the two armies Open
International audience
View article: Navigation through the twists and turns of RNA sequencing technologies: Application to bacterial regulatory RNAs
Navigation through the twists and turns of RNA sequencing technologies: Application to bacterial regulatory RNAs Open
View article: The power of cooperation: Experimental and computational approaches in the functional characterization of bacterial sRNAs
The power of cooperation: Experimental and computational approaches in the functional characterization of bacterial sRNAs Open
Trans‐acting small regulatory RNAs (sRNAs) are key players in the regulation of gene expression in bacteria. There are hundreds of different sRNAs in a typical bacterium, which in contrast to eukaryotic microRNAs are more heterogeneous in …
View article: Pulling the strings of cell cycle: a non-coding RNA, CcnA, modulates the master regulators CtrA and GcrA in <i>Caulobacter crescentus</i>
Pulling the strings of cell cycle: a non-coding RNA, CcnA, modulates the master regulators CtrA and GcrA in <i>Caulobacter crescentus</i> Open
Summary Bacteria are powerful models for understanding how cells divide and accomplish global regulatory programs. In Caulobacter crescentus , a cascade of essential master regulators supervises the correct and sequential activation of DNA…
View article: RsaC sRNA modulates the oxidative stress response of Staphylococcus aureus during manganese starvation
RsaC sRNA modulates the oxidative stress response of Staphylococcus aureus during manganese starvation Open
The human opportunistic pathogen Staphylococcus aureus produces numerous small regulatory RNAs (sRNAs) for which functions are still poorly understood. Here, we focused on an atypical and large sRNA called RsaC. Its length varies between d…