David Mansfield
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View article: Addition of oncolytic virotherapy to clinical isolated limb perfusion for melanoma and sarcoma activates antitumor immunity
Addition of oncolytic virotherapy to clinical isolated limb perfusion for melanoma and sarcoma activates antitumor immunity Open
Background We previously showed that oncolytic virotherapy delivered by isolated limb perfusion (ILP), combined with immune checkpoint inhibition, prevents both local tumor progression and systemic metastases in an animal sarcoma model. Me…
View article: Palbociclib and dsRNA sensor co-operate to enhance anti-cancer effects through ER stress and modulation of immune evasion
Palbociclib and dsRNA sensor co-operate to enhance anti-cancer effects through ER stress and modulation of immune evasion Open
Cytoplasmic pattern recognition receptors (PRR) for double-stranded RNA, such as RIG-I/MDA5, are key mediators of anti-viral responses. Here we screen for synergistic drug-virotherapy combinations and find that the reovirus type III Dearin…
View article: Supplementary Figure 2 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Figure 2 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
Knockdown sequence selection for CXCL12 and proof of modulation of CXCL12 in vitro and in vivo. Primary fibroblasts were modified with lentivirus to overexpress CXCL12 with a FLAG-epitope tag to allow clear detection of CXCL12 at protein l…
View article: Supplementary Figure 1 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Figure 1 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
CD3 expression across time course and examination of CXCL12 expression after irradiation in primary fibroblasts in vitro.
View article: Supplementary Figure 5 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Figure 5 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
Analysis of metastasis in in vivo samples A) Animals were culled at tumour severity limits per model described in figure 5B. Livers, spleens, lungs and ormental mesenteric tissue were harvested for examination of metastasis. Every 10th and…
View article: Supplementary Figure 3 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Figure 3 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
RTOG acute effects after 50 Gy/3 Fr and cumulative later RTOG toxicities for LVSCR/PBS/LVOeCXCL12
View article: Supplementary Table 1 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Table 1 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
Description of RTOG scoring for Acute and Late Toxicities
View article: Data from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Data from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
Radiation-induced fibrosis (RIF) is a progressive pathology deleteriously impacting cancer survivorship. CXCL12 is an immune–stromal signal implicated in fibrosis and innate response. We hypothesized that modulation of CXCL12 would phenoty…
View article: Supplementary Table 2 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Table 2 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
Summary of Western blot density data
View article: Supplementary Figure 4 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Figure 4 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
Flow Cytometry of digested flap tissue: Macrophage subtype at day 90 and T-Cell subtype examination at day 21
View article: Supplementary Table 3 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues
Supplementary Table 3 from CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues Open
Summative Data Table for Differential Gene Expression presented in Figure 4A Heatmap (LVSCR vs. LVShCXCL12 samples)
View article: Whistling in the Wind: The Inevitable Return of Poppy Cultivation to Afghanistan
Whistling in the Wind: The Inevitable Return of Poppy Cultivation to Afghanistan Open
This chapter examines the Taliban’s efforts against drug production and trade in Afghanistan following the Taliban’s new drug edict in October 2023. Utilizing data from satellite imagery analysis, the authors extensive field work in Afghan…
View article: Commentary on Pardo <i>et al</i>.: Whatever the past situation, drug precursor chemicals may in future represent an important source for synthetic drug production in Afghanistan and surrounding countries
Commentary on Pardo <i>et al</i>.: Whatever the past situation, drug precursor chemicals may in future represent an important source for synthetic drug production in Afghanistan and surrounding countries Open
While modelling conducted by Pardo and colleagues points to a need to question the role played by medicines and bulk drug precursor chemicals, we would cautiously conclude that the weight of evidence still supports the view that ephedra ha…
View article: A survey on autonomous environmental monitoring approaches: towards unifying active sensing and reinforcement learning
A survey on autonomous environmental monitoring approaches: towards unifying active sensing and reinforcement learning Open
The environmental pollution caused by various sources has escalated the climate crisis making the need to establish reliable, intelligent, and persistent environmental monitoring solutions more crucial than ever. Mobile sensing systems are…
View article: Durable responses to ATR inhibition with ceralasertib in tumors with genomic defects and high inflammation
Durable responses to ATR inhibition with ceralasertib in tumors with genomic defects and high inflammation Open
BACKGROUNDPhase 1 study of ATRinhibition alone or with radiation therapy (PATRIOT) was a first-in-human phase I study of the oral ATR (ataxia telangiectasia and Rad3-related) inhibitor ceralasertib (AZD6738) in advanced solid tumors.METHOD…
View article: 6 Restricted diet causing irreversible visual impairment in children with autistic spectrum disorder: case series and review
6 Restricted diet causing irreversible visual impairment in children with autistic spectrum disorder: case series and review Open
Though micronutrient deficiency is recognized to cause visual system dysfunction, avoidant/restrictive food intake disorder (ARFID) has been poorly described in relation to this.Review of 18 previously published similar cases highlights th…
View article: Supplementary Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer
Supplementary Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer Open
Supplementary data - revised
View article: Supplementary Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer
Supplementary Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer Open
Supplementary data - revised
View article: Supplementary Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma
Supplementary Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma Open
Supplementary tables and figures
View article: Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer
Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer Open
Purpose:The CANON [CAVATAK in NON–muscle-invasive bladder cancer (NMIBC)] study evaluated a novel ICAM-1–targeted immunotherapeutic-coxsackievirus A21 as a novel oncolytic agent against bladder cancer.Patients and Methods:Fifteen patients …
View article: Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma
Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma Open
Purpose:The prevention and treatment of metastatic sarcoma are areas of significant unmet need. Immune checkpoint inhibitor monotherapy has shown little activity in sarcoma and there is great interest in identifying novel treatment combina…
View article: Supplementary Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma
Supplementary Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma Open
Supplementary tables and figures
View article: Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer
Data from Phase I Trial of an ICAM-1-Targeted Immunotherapeutic-Coxsackievirus A21 (CVA21) as an Oncolytic Agent Against Non Muscle-Invasive Bladder Cancer Open
Purpose:The CANON [CAVATAK in NON–muscle-invasive bladder cancer (NMIBC)] study evaluated a novel ICAM-1–targeted immunotherapeutic-coxsackievirus A21 as a novel oncolytic agent against bladder cancer.Patients and Methods:Fifteen patients …
View article: Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma
Data from PD-1 Blockade Following Isolated Limb Perfusion with Vaccinia Virus Prevents Local and Distant Relapse of Soft-tissue Sarcoma Open
Purpose:The prevention and treatment of metastatic sarcoma are areas of significant unmet need. Immune checkpoint inhibitor monotherapy has shown little activity in sarcoma and there is great interest in identifying novel treatment combina…
View article: ACKNOWLEDGMENTS
ACKNOWLEDGMENTS Open
his book is the result of a long journey enabled and shaped by many people.Most importantly, I want to thank each person in Afghanistan who took the time to talk to me and, through sharing their views, influenced my way of thinking and all…
View article: CDK4/6 inhibition and dsRNA sensor agonism co-operate to enhance anti-cancer effects through ER stress and immune modulation of tumour cells
CDK4/6 inhibition and dsRNA sensor agonism co-operate to enhance anti-cancer effects through ER stress and immune modulation of tumour cells Open
Cytoplasmic pattern recognition receptors (PRRs) for double-stranded RNA (RIG-I/MDA5) are key mediators of anti-viral responses. PRR agonists, such as dsRNA oncolytic Reovirus type 3 Dearing (Rt3D), potently activate RNA sensors. We used a…
View article: OX40 and 4-1BB delineate distinct immune profiles in sarcoma
OX40 and 4-1BB delineate distinct immune profiles in sarcoma Open
Systemic relapse after radiotherapy and surgery is the major cause of disease-related mortality in sarcoma patients. Combining radiotherapy and immunotherapy is under investigation as a means to improve response rates. However, the immune …