Robert David
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View article: Tranexamic acid inhibits group A streptococci–mediated fibrinolysis in vitro and modulates host immune cells in vivo
Tranexamic acid inhibits group A streptococci–mediated fibrinolysis in vitro and modulates host immune cells in vivo Open
Group A Streptococcus (GAS) exploits the host fibrinolytic system by activating plasminogen via streptokinase, promoting clot degradation, tissue invasion, and immune evasion. Tranexamic acid (TXA), a clinically used antifibrinolytic agent…
View article: [68Ga]Ga-NODAGA-RGD post MI reflects activated fibroblasts rather than angiogenesis
[68Ga]Ga-NODAGA-RGD post MI reflects activated fibroblasts rather than angiogenesis Open
Purpose Angiogenesis is crucial in myocardial healing after myocardial infarction (MI). The α v β 3 -integrin, a key regulator of angiogenesis, is targeted by RGD-based PET tracers like [ 68 Ga]Ga-NODAGA-RGD. Yet, angiogenesis imaging usin…
View article: Comparison of Myocardial Function in Young and Old Mice During Acute Myocardial Infarction: A Cardiac Magnetic Resonance Study
Comparison of Myocardial Function in Young and Old Mice During Acute Myocardial Infarction: A Cardiac Magnetic Resonance Study Open
Background/Objectives: This study aimed to compare changes in functional and strain parameters in young and old mice using cardiac MRI before and shortly after myocardial infarction. Methods: In this prospective experimental study, 7 young…
View article: Endurance training in old mice reduces decline of cardiac function after myocardial infarction
Endurance training in old mice reduces decline of cardiac function after myocardial infarction Open
Background Endurance training before the induction of a myocardial infarction (MI) limits scar formation, wall thinning and adverse remodeling after an MI in rodents. The few existing studies have been conducted on young animals, leaving a…
View article: Risk factors associated with urgent ophthalmic reference in diabetic patients
Risk factors associated with urgent ophthalmic reference in diabetic patients Open
A third of the patients require urgent referral. Main causes of urgent referral were DME, severe or worse DR, cataract and high intraocular pressure. Principal risk factors were diabetes duration, systolic blood pressure and HbA1c.
View article: Heart rhythm in vitro: measuring stem cell-derived pacemaker cells on microelectrode arrays
Heart rhythm in vitro: measuring stem cell-derived pacemaker cells on microelectrode arrays Open
Background Cardiac arrhythmias have markedly increased in recent decades, highlighting the urgent need for appropriate test systems to evaluate the efficacy and safety of new pharmaceuticals and the potential side effects of established dr…
View article: BPIFB4 and its longevity-associated haplotype protect from cardiac ischemia in humans and mice
BPIFB4 and its longevity-associated haplotype protect from cardiac ischemia in humans and mice Open
Long-living individuals (LLIs) escape age-related cardiovascular complications until the very last stage of life. Previous studies have shown that a Longevity-Associated Variant (LAV) of the BPI Fold Containing Family B Member 4 ( BPIFB4 )…
View article: New Approaches in Heart Research: Prevention Instead of Cardiomyoplasty?
New Approaches in Heart Research: Prevention Instead of Cardiomyoplasty? Open
Cardiovascular diseases are the leading cause of death in industrialized nations. Due to the high number of patients and expensive treatments, according to the Federal Statistical Office (2017) in Germany, cardiovascular diseases account f…
View article: Cell Programming for Cardiovascular Disease Modeling and Therapy
Cell Programming for Cardiovascular Disease Modeling and Therapy Open
Cardiovascular diseases have a high mortality due to a very limited regenerative potential of lost cardiomyocytes and therefore are one of the leading causes of death in developed countries [...]
View article: The Effects of Hypoxic Preconditioned Murine Mesenchymal Stem Cells on Post-Infarct Arrhythmias in the Mouse Model
The Effects of Hypoxic Preconditioned Murine Mesenchymal Stem Cells on Post-Infarct Arrhythmias in the Mouse Model Open
Ventricular arrhythmias associated with myocardial infarction (MI) have a significant impact on mortality in patients following heart attack. Therefore, targeted reduction of arrhythmia represents a therapeutic approach for the prevention …
View article: Monitoring the maturation of the sarcomere network: a super-resolution microscopy-based approach
Monitoring the maturation of the sarcomere network: a super-resolution microscopy-based approach Open
The in vitro generation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC) is of great importance for cardiac disease modeling, drug-testing applications and for regenerative medicine. Despite the development of var…
View article: Automated annotation of rare-cell types from single-cell RNA-sequencing data through synthetic oversampling
Automated annotation of rare-cell types from single-cell RNA-sequencing data through synthetic oversampling Open
Background The research landscape of single-cell and single-nuclei RNA-sequencing is evolving rapidly. In particular, the area for the detection of rare cells was highly facilitated by this technology. However, an automated, unbiased, and …
View article: Quality control in scRNA-Seq can discriminate pacemaker cells: the mtRNA bias
Quality control in scRNA-Seq can discriminate pacemaker cells: the mtRNA bias Open
Single-cell RNA-sequencing (scRNA-seq) provides high-resolution insights into complex tissues. Cardiac tissue, however, poses a major challenge due to the delicate isolation process and the large size of mature cardiomyocytes. Regardless o…
View article: AMES: Automated evaluation of sarcomere structures in cardiomyocytes
AMES: Automated evaluation of sarcomere structures in cardiomyocytes Open
Background Arrhythmias are severe cardiac diseases and lethal if untreated. To serve as an in vitro drug testing option for anti-arrhythmic agents, cardiomyocytes are being generated in vitro from induced pluripotent stem cells (iPSCs). Un…
View article: 659 Optimizing Telehealth technology in Sleep Medicine at a VA Medical center during the COVID19 pandemic
659 Optimizing Telehealth technology in Sleep Medicine at a VA Medical center during the COVID19 pandemic Open
Introduction The COVID19 pandemic poses an unprecedented challenge for healthcare delivery. Optimization of resources and safety of patients and healthcare workers is crucial. The aim of this study is to analyze impact on resource utilizat…
View article: Single-Cell RNA Sequencing Procedures and Data Analysis
Single-Cell RNA Sequencing Procedures and Data Analysis Open
Single-cell and single-nuclei sequencing experiments reveal previously unseen molecular details. The number of sequencing procedures and computational data analysis approaches have been increasing rapidly in recent years. This chapter prov…
View article: Automated annotation of rare-cell types from single-cell RNA-sequencing data through synthetic oversampling
Automated annotation of rare-cell types from single-cell RNA-sequencing data through synthetic oversampling Open
The research landscape of single-cell and single-nuclei RNA sequencing is evolving rapidly, and one area that is enabled by this technology, is the detection of rare cells. An automated, unbiased and accurate annotation of rare subpopulati…
View article: Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells
Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells Open
The aim of the study was to establish electrical stimulation parameters in order to improve cell growth and viability of human adipose-derived stem cells (hADSC) when compared to non-stimulated cells in vitro. hADSC were exposed to continu…
View article: Printing of vessels for small functional tissues – a preliminary study
Printing of vessels for small functional tissues – a preliminary study Open
Vascularization of bioprinted constructs to ensure sufficient nutrient supply still remains to be a significant task in the tissue engineering community. In order to mimic functional tissue, it is necessary to be able to print vessels in v…
View article: Cardiomyocyte Transplantation after Myocardial Infarction Alters the Immune Response in the Heart
Cardiomyocyte Transplantation after Myocardial Infarction Alters the Immune Response in the Heart Open
We investigated the influence of syngeneic cardiomyocyte transplantation after myocardial infarction (MI) on the immune response and cardiac function. Methods and Results: We show for the first time that the immune response is altered as a…
View article: Dose-Independent Therapeutic Benefit of Bone Marrow Stem Cell Transplantation after MI in Mice
Dose-Independent Therapeutic Benefit of Bone Marrow Stem Cell Transplantation after MI in Mice Open
Several cell populations derived from bone marrow (BM) have been shown to possess cardiac regenerative potential. Among these are freshly isolated CD133+ hematopoietic as well as culture-expanded mesenchymal stem cells. Alternatively, by p…
View article: [68Ga]-NODAGA-RGD Positron Emission Tomography (PET) for Assessment of Post Myocardial Infarction Angiogenesis as a Predictor for Left Ventricular Remodeling in Mice after Cardiac Stem Cell Therapy
[68Ga]-NODAGA-RGD Positron Emission Tomography (PET) for Assessment of Post Myocardial Infarction Angiogenesis as a Predictor for Left Ventricular Remodeling in Mice after Cardiac Stem Cell Therapy Open
Angiogenesis plays a central role in the healing process following acute myocardial infarction. The PET tracer [68Ga]-NODAGA-RGD, which is a ligand for the αvβ3 integrin, has been investigated for imaging angiogenesis in the process of hea…
View article: 18F-FDG PET-Based Imaging of Myocardial Inflammation Following Acute Myocardial Infarction in a Mouse Model
18F-FDG PET-Based Imaging of Myocardial Inflammation Following Acute Myocardial Infarction in a Mouse Model Open
Cellular inflammation is an integral part of the healing process following acute myocardial infarction and has been under intense investigation for both therapeutic and prognostic approaches. Monocytes and macrophages are metabolically hig…
View article: Integrative Cluster Analysis of Whole Hearts Reveals Proliferative Cardiomyocytes in Adult Mice
Integrative Cluster Analysis of Whole Hearts Reveals Proliferative Cardiomyocytes in Adult Mice Open
The recent development and broad application of sequencing techniques at the single-cell level is generating an unprecedented amount of data. The different techniques have their individual limits, but the datasets also offer unexpected pos…