David Potěšil
YOU?
Author Swipe
View article: Proteome Analysis of Seven <i>Treponema pallidum</i> subsp. <i>pallidum</i> Strains Grown <i>In Vitr</i> <i>o</i>
Proteome Analysis of Seven <i>Treponema pallidum</i> subsp. <i>pallidum</i> Strains Grown <i>In Vitr</i> <i>o</i> Open
Treponema pallidum subsp. pallidum (T. pallidum), the fastidious causative agent of syphilis, has become more accessible for research with its recently developed in vitro cultivation method. In this work, the pr…
View article: A Wnt-induced conformational phospho-switch in DVL3 controls interaction with Frizzled receptors and Wnt/β-catenin signaling
A Wnt-induced conformational phospho-switch in DVL3 controls interaction with Frizzled receptors and Wnt/β-catenin signaling Open
Summary When Wnt ligands bind to Frizzled (FZD) receptors, Dishevelled protein (DVL) gets multiphosphorylated by Casein kinase 1 (CK1). Although, it is well known that DVL phosphorylation relays Wnt signals from receptors to downstream eff…
View article: The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3
The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3 Open
The centromere is a specialized domain that facilitates chromosome segregation during mitosis and serves as the site for kinetochore formation. KINETOCHORE NULL2 (αKNL2) is essential for the recognition and loading of the centromeric histo…
View article: Placental mesenchymal stem cells: A promising platform for advancing gene therapy in pancreatic ductal adenocarcinoma
Placental mesenchymal stem cells: A promising platform for advancing gene therapy in pancreatic ductal adenocarcinoma Open
The extreme lethality and limited treatment options for pancreatic ductal adenocarcinoma (PDAC) underscore the urgent need for innovative therapeutic strategies. This study presents the first preclinical investigation of a cell-free gene-d…
View article: Primary bile acid shapes peripheral immunity in inflammatory bowel disease-associated primary sclerosing cholangitis
Primary bile acid shapes peripheral immunity in inflammatory bowel disease-associated primary sclerosing cholangitis Open
Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease often associated with underlying inflammatory bowel disease (IBD). This study investigates how PSC predisposes individuals to altered inflammatory immune responses…
View article: SMG7 and eIF4A constitute a homeostatic module controlling P-body condensation and function of Meiotic bodies
SMG7 and eIF4A constitute a homeostatic module controlling P-body condensation and function of Meiotic bodies Open
Summary Processing bodies (P-bodies) are ribonucleoprotein condensates that regulate RNA processing and storage. Although present constitutively in most cells, their size and composition dynamically change in response to developmental and …
View article: Proteomic Insights into the Adaptation of <i>Acidithiobacillus ferridurans</i> to Municipal Solid Waste Incineration Residues for Enhanced Bioleaching Efficiency
Proteomic Insights into the Adaptation of <i>Acidithiobacillus ferridurans</i> to Municipal Solid Waste Incineration Residues for Enhanced Bioleaching Efficiency Open
Acidithiobacillus spp. have traditionally been utilized to extract metals from mineral ores through bioleaching. This process has recently expanded to include artificial ores, such as those derived from municipal solid waste inciner…
View article: Unveiling the role of sex in the metabolism of indoxyl sulfate and apixaban
Unveiling the role of sex in the metabolism of indoxyl sulfate and apixaban Open
Chronic Kidney Disease (CKD) is associated with heightened risk of thrombosis. Prescription of anticoagulants is key to manage it; however, CKD patients have shown an increased risk of bleeding under anticoagulation therapy compared to non…
View article: <i>Arabidopsis</i> seed stored mRNAs and translation regulation during post-harvest ripening and imbibition
<i>Arabidopsis</i> seed stored mRNAs and translation regulation during post-harvest ripening and imbibition Open
Seed germination marks the critical transition from dormancy to active growth, driven by environmental cues and water availability. This study explores translational regulation during germination by comparing two Arabidopsis thaliana acces…
View article: Contribution of hypoxia-inducible factor 1alpha to pathogenesis of sarcomeric hypertrophic cardiomyopathy
Contribution of hypoxia-inducible factor 1alpha to pathogenesis of sarcomeric hypertrophic cardiomyopathy Open
View article: Proximity interactomics identifies RAI14, EPHA2 and PHACTR4 as essential components of Wnt/planar cell polarity pathway in vertebrates
Proximity interactomics identifies RAI14, EPHA2 and PHACTR4 as essential components of Wnt/planar cell polarity pathway in vertebrates Open
Wnt/planar cell polarity (Wnt/PCP) pathway is an evolutionarily conserved signaling cascade playing an inevitable role in cell biology. Deregulation of Wnt/PCP leads to severe developmental defects or cancer progression. Here, we applied p…
View article: Peripheral players and gut bacteria modulate inflammatory bowel disease phenotype in patients with primary sclerosing cholangitis
Peripheral players and gut bacteria modulate inflammatory bowel disease phenotype in patients with primary sclerosing cholangitis Open
Background & Aims: Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease often associated with underlying inflammatory bowel disease (IBD). We aimed to study how the phenotype in PSC patients compares to IBD alone and…
View article: Unraveling the influence of Indoxyl Sulfate and apixaban in drug metabolism and elimination: Is sex a major player?
Unraveling the influence of Indoxyl Sulfate and apixaban in drug metabolism and elimination: Is sex a major player? Open
Chronic Kidney Disease (CKD) is associated with heightened risk of thrombosis. Prescription of anticoagulants is key to manage it; however, CKD patients have shown an increased risk of bleeding under anticoagulation therapy compared to non…
View article: Carboxy-terminal polyglutamylation regulates signaling and phase separation of the Dishevelled protein
Carboxy-terminal polyglutamylation regulates signaling and phase separation of the Dishevelled protein Open
View article: A hybrid DDA/DIA-PASEF based assay library for a deep proteotyping of triple-negative breast cancer
A hybrid DDA/DIA-PASEF based assay library for a deep proteotyping of triple-negative breast cancer Open
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, and deeper proteome coverage is needed for its molecular characterization. We present comprehensive library of targeted mass spectrometry assays specific…
View article: Fibrotic extracellular matrix impacts cardiomyocyte phenotype and function in an iPSC-derived isogenic model of cardiac fibrosis
Fibrotic extracellular matrix impacts cardiomyocyte phenotype and function in an iPSC-derived isogenic model of cardiac fibrosis Open
View article: Correction: Kolesar et al. Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase. Cells 2022, 11, 165
Correction: Kolesar et al. Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase. Cells 2022, 11, 165 Open
In the original publication [...]
View article: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics
Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics Open
Caspase-9 is traditionally considered the initiator caspase of the intrinsic apoptotic pathway. In the past decade, however, other functions beyond initiation/execution of cell death have been described including cell type-dependent regula…
View article: Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer
Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer Open
High‐grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type of ovarian cancer, ranks among the deadliest malignancies. Many HGSC patients have excess fluid in the peritoneum called ascites. Ascites …
View article: Global Interactome Mapping Reveals Pro-tumorigenic Interactions of NF-κB in Breast Cancer
Global Interactome Mapping Reveals Pro-tumorigenic Interactions of NF-κB in Breast Cancer Open
NF-κB pathway is involved in inflammation; however, recent data shows its role also in cancer development and progression, including metastasis. To understand the role of NF-κB interactome dynamics in cancer, we study the complexity of bre…
View article: An iPSC-derived bio-inspired scaffold modelling the structure and the effects of extracellular matrix in cardiac fibrosis
An iPSC-derived bio-inspired scaffold modelling the structure and the effects of extracellular matrix in cardiac fibrosis Open
Cardiac fibrosis occurs following insults to the myocardium and is characterized by the abnormal accumulation of non-compliant extracellular matrix (ECM), which compromises cardiomyocyte contractile activity and eventually leads to heart f…
View article: Benchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS
Benchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS Open
The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, single-pot solid-phase-enhanced peptide sample prepar…
View article: Unraveling adipose tissue proteomic landscapes in severe obesity: insights into metabolic complications and potential biomarkers
Unraveling adipose tissue proteomic landscapes in severe obesity: insights into metabolic complications and potential biomarkers Open
This study provides insights into distinct proteomic profiles of subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and serum in patients with severe obesity and their associations with clinical traits and body composition. …
View article: Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus Open
Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combin…
View article: #500 Protein composition of extracellular vesicles from malignant ascites defines the key components of tumor microenvironment and predicts prognosis of ovarian cancer patients
#500 Protein composition of extracellular vesicles from malignant ascites defines the key components of tumor microenvironment and predicts prognosis of ovarian cancer patients Open
Introduction/Background High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type of ovarian cancer, ranks among the deadliest malignancies in women. Many HGSC patients present with ascites at the…
View article: Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide
Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide Open
View article: Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m <sup>7</sup> G-cap-dependent translation at the 8- to 16-cell transition
Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m <sup>7</sup> G-cap-dependent translation at the 8- to 16-cell transition Open
Preimplantation mouse embryo development involves temporal–spatial specification and segregation of three blastocyst cell lineages: trophectoderm, primitive endoderm and epiblast. Spatial separation of the outer-trophectoderm lineage from …
View article: A new mechanism of posttranslational polyglutamylation regulates phase separation and signaling of the Wnt pathway protein Dishevelled
A new mechanism of posttranslational polyglutamylation regulates phase separation and signaling of the Wnt pathway protein Dishevelled Open
Polyglutamylation is a reversible post-translational modification that is catalyzed by enzymes from the tubulin tyrosine ligase-like (TTLL) family. Here, we found that TTLL11 generates a previously unknown type of polyglutamylation initiat…
View article: DIA benchmarking dataset (based on Gotti et al., 2021)
DIA benchmarking dataset (based on Gotti et al., 2021) Open
Benchmarking dataset of DIA acquisition methods, based on the manuscript "Extensive and Accurate Benchmarking of DIA Acquisition Methods and Software Tools Using a Complex Proteomic Standard" by Gotti et al. (2021), for the purpose of R vi…
View article: DIA benchmarking dataset (based on Gotti et al., 2021)
DIA benchmarking dataset (based on Gotti et al., 2021) Open
Benchmarking dataset of DIA acquisition methods, based on the manuscript "Extensive and Accurate Benchmarking of DIA Acquisition Methods and Software Tools Using a Complex Proteomic Standard" by Gotti et al. (2021), for the purpose of R vi…