Explanipedia

A Quantitative Model of Chemotherapeutic Drug Sensitivity as a Function of P-Glycoprotein Expression Open

Cara M. Robertus, N. Kannan, David Putnam · 2025

(1) Background: Overexpression of P-glycoprotein (P-gp) is one mediator of multidrug resistance in cancer. While many studies demonstrate the efficacy of modulating P-glycoprotein expression to increase drug response in cancer cells, the n…

Programming immunogenicity of dengue EDIII vaccine antigens using engineered outer membrane vesicles (OMVs) Open

Srinivas Duvvada, Farhan Ahmed, Rafiq Ahmad Khan, Sharul Kamal Abdul Rahim, Saima Naz , et al. · 2025

Dengue is a mosquito-borne viral infection and is more prevalent in the world with no therapeutics and suboptimal vaccine (Dengvaxia) performance against all four serotypes of the dengue virus. Hence, there is an urgent requirement for a n…

Programing Immunogenicity of Dengue EDIII Vaccine Antigens Using Engineered Outer Membrane Vesicles (OMVs) Open

Srinivas Duvvada, Farhan Ahmed, Raeesa Khan, Matin Shaikh Rahim, Saima Naaz , et al. · 2025

Dengue is a mosquito-borne viral infection and is more prevalent in the world with no therapeutics and suboptimal vaccine performance against all four serotypes of the dengue virus. Hence, there is an urgent requirement for a non-infectiou…

Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations<i>in vitro</i>and<i>in vivo</i> Open

Tianzheng Ye, Azmain Alamgir, Cara M. Robertus, Darianna Colina, Connor Monticello , et al. · 2024

Aberrant activation of Wnt signaling results in unregulated accumulation of cytosolic β-catenin, which subsequently enters the nucleus and promotes transcription of genes that contribute to cellular proliferation and malignancy. Here, we s…

Intranasal Vaccination with Recombinant TLR2-Active Outer Membrane Vesicles Containing Sequential M2e Epitopes Protects against Lethal Influenza a Challenge Open

N. Kannan, Annette Choi, Mariela A. Rivera De Jesus, Peter Male Wei, Julie Sahler , et al. · 2024

Influenza is a highly contagious respiratory disease, resulting in an estimated 3 to 5 million cases of severe illness annually. While most influenza vaccines are administered parenterally via injection, one shortcoming is that they do not…

Editorial Board, Aims & Scope, Table of Contents Open

Stefan Spiegel, Conor Doss, Jie Cai, Emily Hu, Ying Jia , et al. · 2023

Arthroplasty for Treating Proximal Femur Metastatic Lesions May Be Associated with Lower Mortality Rates Compared to Intramedullary Nailing within the VA Healthcare System Open

Phillip W. Lam, David Putnam, Marissa M. Song Mayeda, Kenneth R. Gundle · 2023

Metastatic bony disease is a significant health issue, with approximately 700,000 new cases annually that tend to metastasize to bones. The proximal femur in the appendicular skeleton is commonly affected. Our study aimed to investigate mo…

A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens Open

Kevin B. Weyant, Ayomide Oloyede, Sukumar Pal, Julie Liao, Mariela Rivera-De Jesus , et al. · 2023

Imaging optically thick tissues simply and reproducibly: a practical guide to Lightsheet Macroscopy Open

Rebecca M. Williams, Jordana C. Bloom, Cara M. Robertus, Andrew Recknagel, David Putnam , et al. · 2022

Lightsheet microscopy offers an ideal method for imaging of large (mm-cm scale) biological tissues rendered transparent via optical clearing protocols. However the diversity of clearing technologies and tissue types, and how these are adap…

Biological Nanoparticles in Vaccine Development Open

Stephanie M. Curley, David Putnam · 2022

Vaccines represent one of the most successful public health initiatives worldwide. However, despite the vast number of highly effective vaccines, some infectious diseases still do not have vaccines available. New technologies are needed to…

A modular platform for on-demand vaccine self-assembly enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens Open

Kevin B. Weyant, Julie Liao, Mariela Rivera-De Jesus, Thapakorn Jaroentomeechai, Tyler D. Moeller , et al. · 2021

Engineered outer membrane vesicles (OMVs) derived from laboratory strains of bacteria are a promising technology for the creation of non-infectious, nanoparticle vaccines against diverse pathogens. As mimics of the bacterial cell surface, …

Editorial Board, Aims & Scope, Table of Contents Open

Stefan Spiegel, Conor Doss, Jie Cai, Emily Hu, Ying Jia , et al. · 2020

Coagulation performance of cationic

Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis Open

Yehou M.D. Gnopo, Aditya Misra, Hung-Lun Hsu, Matthew P. DeLisa, Susan Daniel , et al. · 2020

A lipid mixing assay to accurately quantify the fusion of outer membrane vesicles Open

Yehou M.D. Gnopo, David Putnam · 2019

Selective and Tunable Galectin Binding of Glycopolymers Synthesized by a Generalizable Conjugation Method Open

Zhou Can, Heidi L. Reesink, David Putnam · 2019

Glycopolymers, conjugates of synthetic polymers with pendant carbohydrates, are becoming increasingly important to probe the role of carbohydrates in cellular processes and for applications like biosensors and drug delivery. A library of g…

Boundary mode lubrication of articular cartilage with a biomimetic diblock copolymer Open

Zhexun Sun, Elizabeth Feeney, Ya Guan, Sierra G. Cook, Delphine Gourdon , et al. · 2019

Significance Lubricin is a glycoprotein that protects cartilage in weight-bearing joints under boundary mode conditions, which defines the conditions under which physical cartilage damage takes place. The natural expression of lubricin dec…

Michael L. Shuler Tribute Open

Michael Shuler Tribute, Douglas S. Clark, Claudia Fischbach, Daniel A. Hammer, Kelvin H. Lee , et al. · 2018

lives of countless individuals.From the undergraduates who learned the fundamentals of bioprocess engineering and nanobiotechnology, to the graduate students who helped push knowledge boundaries across length scales, to the young

Beneficial Effects of Exercise on Subendothelial Matrix Stiffness Are Short-Lived Open

Julie C. Kohn, François Bordeleau, Joseph P. Miller, Hannah C. Watkins, Shweta Modi , et al. · 2018

Aerobic exercise helps to maintain cardiovascular health in part by mitigating age-induced arterial stiffening. However, the long-term effects of exercise regimens on aortic stiffness remain unknown, especially in the intimal extracellular…

Immunization with outer membrane vesicles displaying conserved surface polysaccharide antigen elicits broadly antimicrobial antibodies Open

Taylor C. Stevenson, Colette Cywes‐Bentley, Tyler D. Moeller, Kevin B. Weyant, David Putnam , et al. · 2018

Significance A broad range of bacterial, fungal, and protozoan cells produce the surface polysaccharide poly- N -acetyl- d -glucosamine (PNAG), making this antigen an attractive target for vaccination against multiple human and economicall…

Synergistic Interactions of a Synthetic Lubricin-Mimetic with Fibronectin for Enhanced Wear Protection Open

Roberto C. Andresen Eguiluz, Sierra G. Cook, Mingchee Tan, Cory N. Brown, Noah Pacifici , et al. · 2017

Lubricin (LUB), a major mucinous glycoprotein of mammalian synovial fluids, is believed to provide excellent lubrication to cartilage surfaces. Consequently, when joint disease or replacement leads to increased friction and surface damage …

Safe Recombinant Outer Membrane Vesicles that Display M2e Elicit Heterologous Influenza Protection Open

Hannah C. Watkins, C. Garrett Rappazzo, Jaclyn S. Higgins, Xiangjie Sun, Nicole Brock , et al. · 2017

Recombinant, Escherichia coli-derived outer membrane vesicles (rOMVs), which display heterologous protein subunits, have potential as a vaccine adjuvant platform. One drawback to rOMVs is their lipopolysaccharide (LPS) content, limiting th…

Binding and lubrication of biomimetic boundary lubricants on articular cartilage Open

Kirk J. Samaroo, Mingchee Tan, David Putnam, Lawrence J. Bonassar · 2016

The glycoprotein, lubricin, is the primary boundary lubricant of articular cartilage and has been shown to prevent cartilage damage after joint injury. In this study, a library of eight bottle‐brush copolymers were synthesized to mimic the…

Outer membrane vesicles displaying engineered glycotopes elicit protective antibodies Open

Linxiao Chen, Jenny L. Valentine, Chung‐Jr Huang, Christine E. Endicott, Tyler D. Moeller , et al. · 2016

Significance Conjugate vaccines have proven to be an effective and safe strategy for reducing the incidence of disease caused by bacterial pathogens. However, the manufacture of these vaccines is technically demanding, inefficient, and exp…

Immunization with Outer Membrane Vesicles Displaying Designer Glycotopes Yields Class-Switched, Glycan-Specific Antibodies Open

Jenny L. Valentine, Linxiao Chen, Emily C. Perregaux, Kevin B. Weyant, Joseph Rosenthal , et al. · 2016

Synthetic Biomaterials from Metabolically Derived Synthons Open

Nicole G. Ricapito, Cynthia Ghobril, Heng Zhang, Mark W. Grinstaff, David Putnam · 2016

The utility of metabolic synthons as the building blocks for new biomaterials is based on the early application and success of hydroxy acid based polyesters as degradable sutures and controlled drug delivery matrices. The sheer number of p…

A Simple and Sensitive Method to Quantify Biodegradable Nanoparticle Biodistribution using Europium Chelates Open

Lindsey Crawford, Jaclyn S. Higgins, David Putnam · 2015

The biodistribution of biodegradable nanoparticles can be difficult to quantify. We report a method using time resolved fluorescence (TRF) from a lanthanide chelate to minimize background autofluorescence and maximize the signal to noise r…

David Putnam YOU? Author Swipe