David I. Roper
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View article: Author response: Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division
Author response: Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division Open
View article: Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division
Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division Open
Bacterial cell division relies on precise peptidoglycan (PG) remodelling, a process orchestrated by the FtsEX complex. Comprised of FtsE and FtsX, this complex collaborates with EnvC, a periplasmic lytic enzyme activator, to regulate septa…
View article: Discovery and chemical optimisation of a potent, Bi-cyclic antimicrobial inhibitor of Escherichia coli PBP3
Discovery and chemical optimisation of a potent, Bi-cyclic antimicrobial inhibitor of Escherichia coli PBP3 Open
Penicillin binding proteins (PBPs) are well validated antimicrobial targets, but the prevalence of β-lactamase driven resistance and, more rarely, target-based mutations, necessitates new classes of PBP-targeting drugs. Here we describe th…
View article: Regulation of the essential peptidoglycan hydrolytic complex FtsEX-PcsB during <i>Streptococcus pneumoniae</i> cell division
Regulation of the essential peptidoglycan hydrolytic complex FtsEX-PcsB during <i>Streptococcus pneumoniae</i> cell division Open
In the human commensal Gram-positive bacterial pathogen Streptococcus pneumoniae, the essential extracellular cell-division-associated peptidoglycan (PG) hydrolase PcsB interacts directly with the cytoplasmic-membrane-bound complex between…
View article: Delivery determinants of an <i>Acinetobacter baumannii</i> type VI secretion system bifunctional peptidoglycan hydrolase
Delivery determinants of an <i>Acinetobacter baumannii</i> type VI secretion system bifunctional peptidoglycan hydrolase Open
Acinetobacter baumannii is a Gram-negative opportunistic pathogen and is a common cause of nosocomial infections. The increasing development of antibiotic resistance in this organism is a global health concern. The A. baumannii clinical is…
View article: Characterization of a GpsB-associated regulator of PBP1a reveals the organization of the cell wall remodeling complex of<i>Streptococcus pneumoniae</i>
Characterization of a GpsB-associated regulator of PBP1a reveals the organization of the cell wall remodeling complex of<i>Streptococcus pneumoniae</i> Open
SUMMARY Class A PBPs (aPBPs) play a key role in the biosynthesis and remodeling of peptidoglycan, the main component of the bacterial cell wall. The human bacterial pathogen Streptococcus pneumoniae produces three aPBPs, which are regulate…
View article: Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline
Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline Open
The use of membrane-specific dyes for in vivo fluorescent microscopy is commonplace. However, most of these reagents are non-specific and cannot track specific lipid species movement, instead often acting as non-covalent lipid-associated p…
View article: Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline
Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline Open
The use of membrane-specific dyes for in vivo fluorescent microscopy is commonplace. However, most of these reagents are non-specific and cannot track specific lipid species movement, instead often acting as non-covalent lipid associated p…
View article: Delivery determinants of an<i>Acinetobacter baumannii</i>type VI secretion system bifunctional peptidoglycan hydrolase
Delivery determinants of an<i>Acinetobacter baumannii</i>type VI secretion system bifunctional peptidoglycan hydrolase Open
Acinetobacter baumannii is a Gram-negative opportunistic pathogen that is a common cause of nosocomial infections. The increasing development of antibiotic resistance in this organism is a global health concern. The A. baumannii clinical i…
View article: Discovery and chemical optimisation of a Potent, Bi-cyclic (Bicycle<sup>®</sup>) Antimicrobial Inhibitor of <i>Escherichia coli</i> PBP3
Discovery and chemical optimisation of a Potent, Bi-cyclic (Bicycle<sup>®</sup>) Antimicrobial Inhibitor of <i>Escherichia coli</i> PBP3 Open
Penicillin binding proteins (PBPs) are well validated antimicrobial targets, but the prevalence of β-lactamase driven resistance and, more rarely, target-based mutations, necessitates new classes of PBP-targeting drugs. Here we describe th…
View article: Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division
Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division Open
Bacterial cell division relies on precise peptidoglycan (PG) remodelling, a process orchestrated by the FtsEX complex. Comprised of FtsE and FtsX, this complex collaborates with EnvC, a periplasmic lytic enzyme activator, to regulate septa…
View article: Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division
Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division Open
Bacterial cell division relies on precise peptidoglycan (PG) remodelling, a process orchestrated by the FtsEX complex. Comprised of FtsE and FtsX, this complex collaborates with EnvC, a periplasmic lytic enzyme activator, to regulate septa…
View article: Structural insights into peptidoglycan hydrolysis by the FtsEX system in <i>Escherichia coli</i> during cell division
Structural insights into peptidoglycan hydrolysis by the FtsEX system in <i>Escherichia coli</i> during cell division Open
Bacterial cell division relies on precise peptidoglycan (PG) remodelling, a process orchestrated by the FtsEX complex. Comprised of FtsE and FtsX, this complex collaborates with EnvC, a periplasmic lytic enzyme activator, to regulate septa…
View article: Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex Open
View article: Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline
Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline Open
The use of membrane-specific dyes for in vivo fluorescent microscopy is commonplace. However, most of these reagents are non-specific and cannot track specific lipid species movement, instead often acting as non-covalent lipid associated p…
View article: Activator-induced conformational changes regulate division-associated peptidoglycan amidases
Activator-induced conformational changes regulate division-associated peptidoglycan amidases Open
AmiA and AmiB are peptidoglycan-hydrolyzing enzymes from Escherichia coli that are required to break the peptidoglycan layer during bacterial cell division and maintain integrity of the cell envelope. In vivo, the activity of AmiA and AmiB…
View article: A conserved zinc-binding site in <i>Acinetobacter baumannii</i> PBP2 required for elongasome-directed bacterial cell shape
A conserved zinc-binding site in <i>Acinetobacter baumannii</i> PBP2 required for elongasome-directed bacterial cell shape Open
Acinetobacter baumannii is a gram-negative bacterial pathogen that causes challenging nosocomial infections. β-lactam targeting of penicillin-binding protein (PBP)–mediated cell wall peptidoglycan (PG) formation is a well-established antim…
View article: Investigating the effect of bacteriophages on bacterial FtsZ localisation
Investigating the effect of bacteriophages on bacterial FtsZ localisation Open
Escherichia coli is one of the most common Gram-negative pathogens and is responsible for infection leading to neonatal meningitis and sepsis. The FtsZ protein is a bacterial tubulin homolog required for cell division in most species, incl…
View article: Investigation of the Localisation of FtsZ in Pathogenic E. coli Upon Bacteriophage Addition in a Human Cell Model as a Biomarker for Antibacterial Agents
Investigation of the Localisation of FtsZ in Pathogenic E. coli Upon Bacteriophage Addition in a Human Cell Model as a Biomarker for Antibacterial Agents Open
Antimicrobial resistance is a growing problem worldwide and has created a need for novel antibacterial agents and strategies. Escherichia coli is one of the most common Gram-negative pathogens and is responsible for infection leading to ne…
View article: Plant peptidoglycan precursor biosynthesis: Conservation between moss chloroplasts and Gram-negative bacteria
Plant peptidoglycan precursor biosynthesis: Conservation between moss chloroplasts and Gram-negative bacteria Open
Accumulating evidence suggests that peptidoglycan, consistent with a bacterial cell wall, is synthesized around the chloroplasts of many photosynthetic eukaryotes, from glaucophyte algae to early-diverging land plants including pteridophyt…
View article: Structural basis of lipopolysaccharide maturation by the O-antigen ligase
Structural basis of lipopolysaccharide maturation by the O-antigen ligase Open
View article: Plant peptidoglycan precursor biosynthesis: Conservation between moss chloroplasts and Gram negative bacteria
Plant peptidoglycan precursor biosynthesis: Conservation between moss chloroplasts and Gram negative bacteria Open
An accumulation of evidence suggests that peptidoglycan, consistent with a bacterial cell wall, is synthesised around the chloroplasts of many photosynthetic eukaryotes, from glaucophyte algae to land plants at least as evolved as pteridop…
View article: A Dynamic Network of Proteins Facilitate Cell Envelope Biogenesis in Gram-Negative Bacteria
A Dynamic Network of Proteins Facilitate Cell Envelope Biogenesis in Gram-Negative Bacteria Open
Bacteria must maintain the ability to modify and repair the peptidoglycan layer without jeopardising its essential functions in cell shape, cellular integrity and intermolecular interactions. A range of new experimental techniques is bring…
View article: The Pneumococcal Divisome: Dynamic Control of Streptococcus pneumoniae Cell Division
The Pneumococcal Divisome: Dynamic Control of Streptococcus pneumoniae Cell Division Open
Cell division in Streptococcus pneumoniae (pneumococcus) is performed and regulated by a protein complex consisting of at least 14 different protein elements; known as the divisome. Recent findings have advanced our understanding of the mo…
View article: A molecular link between cell wall biosynthesis, translation fidelity, and stringent response in <i>Streptococcus pneumoniae</i>
A molecular link between cell wall biosynthesis, translation fidelity, and stringent response in <i>Streptococcus pneumoniae</i> Open
Significance During infection, microbes must survive the hostile environmental conditions of the human host. When exposed to stresses, bacteria activate an intracellular response, known as the stringent response pathway, to ensure their su…
View article: Structure-based modeling and dynamics of MurM, a Streptococcus pneumoniae penicillin resistance determinant present at the cytoplasmic membrane
Structure-based modeling and dynamics of MurM, a Streptococcus pneumoniae penicillin resistance determinant present at the cytoplasmic membrane Open
View article: Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation Open
The Gram-negative outer-membrane envelops the bacterium and functions as a permeability barrier against antibiotics, detergents, and environmental stresses. Some virulence factors serve to maintain the integrity of the outer membrane, incl…
View article: Author response: Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Author response: Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation Open
View article: Insights into bacterial cell division from a structure of EnvC bound to the FtsX periplasmic domain
Insights into bacterial cell division from a structure of EnvC bound to the FtsX periplasmic domain Open
Significance The peptidoglycan layer is a core component of the bacterial cell envelope that provides a barrier to the environment and protection from osmotic shock. During division, bacteria must break and rebuild the peptidoglycan layer …
View article: Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation Open
The Gram-negative outer membrane envelops the bacterium and functions as a permeability barrier against antibiotics, detergents and environmental stresses. Some virulence factors serve to maintain the integrity of the outer membrane, inclu…