David Scheerer
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View article: Interplay between conformational dynamics and substrate binding regulates enzymatic activity: a single-molecule FRET study
Interplay between conformational dynamics and substrate binding regulates enzymatic activity: a single-molecule FRET study Open
Single-molecule FRET reveals urea-induced conformational shifts in adenylate kinase, enhancing activity through promoting the open conformation and efficient substrate positioning.
View article: Enzyme activation by urea reveals the interplay between conformational dynamics and substrate binding: a single-molecule FRET study
Enzyme activation by urea reveals the interplay between conformational dynamics and substrate binding: a single-molecule FRET study Open
Proteins often harness extensive motions of domains and subunits to promote their function. Deciphering how these movements impact activity is key for understanding life’s molecular machinery. The enzyme adenylate kinase is an intriguing e…
View article: Allosteric communication between ligand binding domains modulates substrate inhibition in adenylate kinase
Allosteric communication between ligand binding domains modulates substrate inhibition in adenylate kinase Open
Enzymes play a vital role in life processes; they control chemical reactions and allow functional cycles to be synchronized. Many enzymes harness large-scale motions of their domains to achieve tremendous catalytic prowess and high selecti…
View article: Allosteric communication between ligand binding domains modulates substrate inhibition in adenylate kinase
Allosteric communication between ligand binding domains modulates substrate inhibition in adenylate kinase Open
Enzymes play a vital role in life processes; they control chemical reactions and allow functional cycles to be synchronized. Many enzymes harness large-scale motions of their domains to achieve tremendous catalytic prowess and high selecti…
View article: Role of Repeated Conformational Transitions in Substrate Binding of Adenylate Kinase
Role of Repeated Conformational Transitions in Substrate Binding of Adenylate Kinase Open
The catalytic cycle of the enzyme adenylate kinase involves large conformational motions between open and closed states. A previous single-molecule experiment showed that substrate binding tends to accelerate both the opening and the closi…
View article: Control of protein activity by photoinduced spin polarized charge reorganization
Control of protein activity by photoinduced spin polarized charge reorganization Open
Considerable electric fields are present within living cells, and the role of bioelectricity has been well established at the organismal level. Yet much remains to be learned about electric-field effects on protein function. Here, we use p…
View article: Control of protein activity by photoinduced spin polarized charge reorganization
Control of protein activity by photoinduced spin polarized charge reorganization Open
Considerable electric fields are present within living cells, and the role of bioelectricity has been well established at the organismal level. Yet little is known about electric-field effects on protein function. Here we use phototriggere…
View article: Urea as a Protein LID Opener: Overcoming Substrate Inhibition in Adenylate Kinase by Tuning Conformational Dynamics
Urea as a Protein LID Opener: Overcoming Substrate Inhibition in Adenylate Kinase by Tuning Conformational Dynamics Open
View article: Frontispiece: Enhanced Sensitivity to Local Dynamics in Peptides by Use of Temperature‐Jump IR Spectroscopy and Isotope Labeling
Frontispiece: Enhanced Sensitivity to Local Dynamics in Peptides by Use of Temperature‐Jump IR Spectroscopy and Isotope Labeling Open
Site-selected dynamics of a three-stranded β-sheet peptide were probed by laser-excited temperature-jump infrared spectroscopy and isotopic labeling. Relaxation time constants showed an enhanced sensitivity to vibrational coupling. For mor…
View article: Enhanced Sensitivity to Local Dynamics in Peptides by use of Temperature-Jump IR-Spectroscopy and Isotope Labeling
Enhanced Sensitivity to Local Dynamics in Peptides by use of Temperature-Jump IR-Spectroscopy and Isotope Labeling Open
View article: Enhanced Sensitivity to Local Dynamics in Peptides by Use of Temperature‐Jump IR Spectroscopy and Isotope Labeling
Enhanced Sensitivity to Local Dynamics in Peptides by Use of Temperature‐Jump IR Spectroscopy and Isotope Labeling Open
Site‐specific isotopic labeling of molecules is a widely used approach in IR spectroscopy to resolve local contributions to vibrational modes. The induced frequency shift of the corresponding IR band depends on the substituted masses, as w…
View article: Isotopically Edited Vibrational Spectra and Dynamics for Three-Strand B-Sheet Peptides. DFT Spectral and MD Dynamics Simulations
Isotopically Edited Vibrational Spectra and Dynamics for Three-Strand B-Sheet Peptides. DFT Spectral and MD Dynamics Simulations Open
View article: Investigation of peptide dynamics by means of time-resolved IR-laser spectroscopy
Investigation of peptide dynamics by means of time-resolved IR-laser spectroscopy Open
β-Sheet-containing structures are ubiquitous for many misfolded protein states. In regard to the importance of aggregates and fibrils for the onset of neurodegenerative diseases like Parkinson and Alzheimer, a better understanding of the m…
View article: Spectra and Simulation of Model Beta-Sheets and Hairpins. Impact of Turn Sequences and Aromatic Contacts on Equilibria and Dynamics
Spectra and Simulation of Model Beta-Sheets and Hairpins. Impact of Turn Sequences and Aromatic Contacts on Equilibria and Dynamics Open
View article: Three-Strand β-Sheet Peptide Models. IR, VCD and ECD SPECTRA, T-Jump Dynamics and NMR Structures Support MD and DFT Simulatations
Three-Strand β-Sheet Peptide Models. IR, VCD and ECD SPECTRA, T-Jump Dynamics and NMR Structures Support MD and DFT Simulatations Open