David Scheuring
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Salicylic acid: new pathways arising? Open
While the role of salicylic acid (SA) for plant immunity has been investigated for decades, its function in regulating plant growth and development has only come into focus recently. Several studies indicate that SA – auxin crosstalk plays…
Salicylic acid inhibits V-ATPase activity and restricts cell elongation Open
While the role of salicylic acid (SA) in plant defence has been investigated for decades, its role in regulating plant growth and development has only come into focus recently. SA application inhibits growth independently of the establishe…
ERAD machinery controls the conditional turnover of PIN-LIKES in plants Open
Auxin is a crucial phytohormone that regulates plant development and facilitates dynamic responses to environmental changes through subcellular control mechanisms. PIN-LIKES (PILS) are auxin transport facilitators at the endoplasmic reticu…
ERAD machinery controls the conditional turnover of PIN-LIKES in plants Open
Auxin is a crucial phytohormone that regulates plant development and facilitates dynamic responses to environmental changes through subcellular control mechanisms. PIN-LIKES (PILS) are auxin transport facilitators at the endoplasmic reticu…
In memoriam: David G. Robinson Open
We are deeply saddened to report that David Gordon Robinson passed away on Tuesday, 5 November 2024. He has left behind his wife and three children. Without doubt, David was one of Europe’s leading plant cell biologists and electron micros…
View article: Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals TEF5 as an early PSII assembly factor
Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals TEF5 as an early PSII assembly factor Open
PSII assembly requires auxiliary factors, including Psb28. Although the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas (Chlamydomonas reinhardtii) psb28 null mutant is severely imp…
Light at the end of the tunnel: FRAP assays combined with super resolution microscopy confirm the presence of a tubular vacuole network in meristematic plant cells Open
Plant vacuoles play key roles in cellular homeostasis, performing catabolic and storage functions, and regulating pH and ion balance. Despite their essential role, there is still no consensus on how vacuoles are established. A model propos…
At knifepoint: Appressoria-dependent turgor pressure of filamentous plant pathogens Open
Filamentous pathogens need to overcome plant barriers for successful infection. To this end, special structures, most commonly appressoria, are used for penetration. In differentiated appressoria, the generation of high turgor pressure is …
Salicylic Acid restricts cell elongation and induces changes of vacuolar morphology and pH Open
The phytohormone salicylic acid (SA) is a key factor to balance plant defence as well as growth and development. While its role in plant defence has been investigated for decades, regulation of plant growth and development has only come in…
Plant infection by the necrotrophic fungus <i>Botrytis</i> requires actin‐dependent generation of high invasive turgor pressure Open
Summary The devastating pathogen Botrytis cinerea infects a broad spectrum of host plants, causing great socio‐economic losses. The necrotrophic fungus rapidly kills plant cells, nourishing their wall and cellular contents. To this end, ne…
View article: Complexome profiling of the Chlamydomonas<i>psb28</i>mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor
Complexome profiling of the Chlamydomonas<i>psb28</i>mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor Open
Several auxiliary factors are required for the assembly of photosystem (PS) II, one of which is Psb28. While the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas psb28 -null mutant i…
View article: AtVPS13M1 is involved in lipid remodeling in low phosphate and is located at the mitochondria surface in plants
AtVPS13M1 is involved in lipid remodeling in low phosphate and is located at the mitochondria surface in plants Open
VPS13 are conserved lipid transporters with multiple subcellular localizations playing key roles in many fundamental cellular processes. While the localization and function of VPS13 have been extensively investigated in yeast and animals, …
Genome Comparisons between Botrytis fabae and the Closely Related Gray Mold Fungus Botrytis cinerea Reveal Possible Explanations for Their Contrasting Host Ranges Open
While Botrytis cinerea causes gray mold on many plants, its close relative, Botrytis fabae, is host-specifically infecting predominantly faba bean plants. To explore the basis for its narrow host range, a gapless genome sequence of B. faba…
Plant infection by the necrotrophic fungus<i>Botrytis</i>requires actin-dependent generation of high invasive turgor pressure Open
The devastating pathogen Botrytis cinerea infects a broad spectrum of host plants, causing great socio-economic losses. The necrotrophic fungus rapidly kills plant cells, nourishing their walls and cellular contents. To this end, necrotrop…
Networked proteins redundantly interact with VAP27 and RABG3 to regulate membrane tethering at the vacuole and beyond Open
Biological processes in eukaryotes depend on the spatio-temporal compartmentalization of their cells. Integrity and positioning of organelles on the other hand rely on the organization of the actin cytoskeleton. Previously, it has been sho…
CLPB3 is required for the removal of chloroplast protein aggregates and thermotolerance in <i>Chlamydomonas</i> Open
In the cytosol of plant cells, heat-induced protein aggregates are resolved by the CASEIN LYTIC PROTEINASE/HEAT SHOCK PROTEIN 100 (CLP/HSP100) chaperone family member HSP101, which is essential for thermotolerance. For the chloroplast fami…
<i>Botrytis</i> hypersensitive response inducing protein 1 triggers noncanonical PTI to induce plant cell death Open
According to their lifestyle, plant pathogens are divided into biotrophic and necrotrophic organisms. Biotrophic pathogens exclusively nourish living host cells, whereas necrotrophic pathogens rapidly kill host cells and nourish cell walls…
CLPB3 is required for the removal of chloroplast protein aggregates and for thermotolerance in Chlamydomonas Open
In the cytosol of plant cells, heat-induced protein aggregates are resolved by ClpB/Hsp100 family member HSP101, which is essential for thermotolerance. For chloroplast family member CLPB3 this is less clear with controversial reports on i…
Multiple knockout mutants reveal a high redundancy of phytotoxic compounds contributing to necrotrophic pathogenesis of Botrytis cinerea Open
Botrytis cinerea is a major plant pathogen infecting more than 1400 plant species. During invasion, the fungus rapidly kills host cells, which is believed to be supported by induction of programmed plant cell death. To comprehensively eval…
Fast and global reorganization of the chloroplast protein biogenesis network during heat acclimation Open
Photosynthesis is a central determinant of plant biomass production, but its homeostasis is increasingly challenged by heat. Little is known about the sensitive regulatory principles involved in heat acclimation that underly the biogenesis…
Vacuolar fructose transporter SWEET17 is critical for root development and drought tolerance Open
Root growth and architecture are markedly influenced by both developmental and environmental cues. Sugars integrate different stimuli and are essential building blocks and signaling molecules for modulating the root system. Members from th…
Multiple knockout mutants reveal a high redundancy of phytotoxic compounds that determine necrotrophic pathogenesis of <i>Botrytis cinerea</i> Open
Botrytis cinerea is a major pathogen of more than 1400 plant species. During infection, the kills host cells during infection and spreads through necrotic tissue, which is believed to be supported by induction of programmed plant cell deat…
Differential degradation of RNA species by autophagy-related pathways in Arabidopsis Open
The plant vacuole recycles proteins and RNA delivered to it by autophagy. In this study, by isolating intact vacuoles from Arabidopsis plants, followed by subsequent RNA purification, and deep sequencing, we provide a comprehensive charact…
Real-time monitoring of subcellular H2O2 distribution in <i>Chlamydomonas reinhardtii</i> Open
Hydrogen peroxide (H2O2) is recognized as an important signaling molecule in plants. We sought to establish a genetically encoded, fluorescent H2O2 sensor that allows H2O2 monitoring in all major subcompartments of a Chlamydomonas cell. To…
Light at the end of the tunnel: FRAP assay reveals that plant vacuoles start as a tubular network Open
Plant vacuoles play key roles in cellular homeostasis performing catabolic and storage functions, regulating pH and ion balance. The essential role of vacuoles for plant cell viability makes them a notoriously difficult subject to study im…
Vacuolar occupancy is crucial for cell elongation and growth regardless of the underlying mechanism Open
In the physiological range, the phytohormone auxin inhibits the growth of underground tissues. In the roots of Arabidopsis thaliana, cell size inhibition has been shown to be accompanied by auxin-mediated reduction of vacuole size. A tonop…
CTP Synthase 2 From Arabidopsis thaliana Is Required for Complete Embryo Development Open
Pyrimidine de novo synthesis is an essential pathway in all organisms. The final and rate-limiting step in the synthesis of the nucleotide cytidine triphosphate (CTP) is catalyzed by CTP synthase (CTPS), and Arabidopsis harbors five isofor…
CTP-Synthase 2 from<i>Arabidopsis thaliana</i>is required for complete embryo development Open
Pyrimidine de novo synthesis is an essential pathway in all organisms. The final and rate limiting step in the synthesis of the nucleotide CTP is catalyzed by CTP-Synthase (CTPS) and Arabidopsis harbors five isoforms. Single knockouts of e…
The secreted hypersensitive response inducing protein 1 from<i>Botrytis cinerea</i>displays non-canonical PAMP-activity Open
According to their lifestyle, plant pathogens are divided into biotrophic and necrotrophic organisms. While biotrophic pathogens establish a relationship with living host cells, necrotrophic pathogens rapidly kill host cells and feed on th…