David I. Stuart
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View article: <i>In situ</i> chromatin dynamics and HIV-1 nuclear trafficking
<i>In situ</i> chromatin dynamics and HIV-1 nuclear trafficking Open
The organization and dynamics of chromatin within intact nuclei remain poorly defined, limiting understanding of how nuclear architecture influences macromolecular transport. HIV-1 provides a striking example of a large complex that must t…
View article: Evidence on mpox clade Ia and clade Ib: A Rapid Research Needs Appraisal
Evidence on mpox clade Ia and clade Ib: A Rapid Research Needs Appraisal Open
The identified evidence-gaps provide a baseline to inform coordinated research strategies to generate evidence for clinical and public health responses to improve outcomes.
View article: Electron fourier ptychography for phase reconstruction
Electron fourier ptychography for phase reconstruction Open
Exit wavefunction reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive …
View article: Assessing the Possibility of Recurrent Diabetic Foot Ulcer Prevention via Remote Patient Monitoring: A Feasibility Study
Assessing the Possibility of Recurrent Diabetic Foot Ulcer Prevention via Remote Patient Monitoring: A Feasibility Study Open
Aims Our main aims in this study of diabetic patients at risk of foot ulcers were to evaluate: (a) adherence to the use of an at‐home thermal and visual digital foot scanner, (b) the feasibility of utilising thermovisual scan data to perfo…
View article: Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development
Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development Open
Influenza virus neuraminidase (NA) is a crucial target for protective antibodies, yet the development of recombinant NA protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: Capsid Restructuring Activates Semi-Conservative dsRNA Transcription in Cystovirus ɸ6
Capsid Restructuring Activates Semi-Conservative dsRNA Transcription in Cystovirus ɸ6 Open
Summary Double-stranded (ds)RNA viruses replicate and transcribe their genome within a proteinaceous viral capsid to evade host cell defenses. While Reovirale s members use conservative transcription, most dsRNA viruses, including cystovir…
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza virus neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approac…
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development
Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development Open
Influenza virus neuraminidase (NA) is a crucial target for protective antibodies, yet the development of recombinant NA protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: Electron Fourier ptychography for phase reconstruction
Electron Fourier ptychography for phase reconstruction Open
Phase reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive materials. W…
View article: <i>TomoCPT</i>: a generalizable model for 3D particle detection and localization in cryo-electron tomograms
<i>TomoCPT</i>: a generalizable model for 3D particle detection and localization in cryo-electron tomograms Open
Cryo-electron tomography is a rapidly developing field for studying macromolecular complexes in their native environments and has the potential to revolutionize our understanding of protein function. However, fast and accurate identificati…
View article: From Voxels to Viruses: Using Deep Learning and Crowdsourcing to Understand a Virus Factory
From Voxels to Viruses: Using Deep Learning and Crowdsourcing to Understand a Virus Factory Open
Many bioimaging research projects require objects of interest to be identified, located, and then traced to allow quantitative measurement. Depending on the complexity of the system and imaging, instance segmentation is often done manually…
View article: A broadly reactive ultralong bovine antibody that can determine the integrity of foot-and-mouth disease virus capsids
A broadly reactive ultralong bovine antibody that can determine the integrity of foot-and-mouth disease virus capsids Open
Foot-and-mouth disease vaccination using inactivated virus is suboptimal, as the icosahedral viral capsids often disassemble into antigenically distinct pentameric units during long-term storage, or exposure to elevated temperature or lowe…
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza virus neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approac…
View article: Oligomerization-driven avidity correlates with SARS-CoV-2 cellular binding and inhibition
Oligomerization-driven avidity correlates with SARS-CoV-2 cellular binding and inhibition Open
Cellular processes are controlled by the thermodynamics of the underlying biomolecular interactions. Frequently, structural investigations use one monomeric binding partner, while ensemble measurements of binding affinities generally yield…
View article: Concerted deletions eliminate a neutralizing supersite in SARS-CoV-2 BA.2.87.1 spike
Concerted deletions eliminate a neutralizing supersite in SARS-CoV-2 BA.2.87.1 spike Open
BA.2.87.1 represents a major shift in the BA.2 lineage of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is unusual in having two lengthy deletions of polypeptide in the spike (S) protein, one of which removes a beta-stra…
View article: The effect of pH on the structure of Bluetongue virus VP5
The effect of pH on the structure of Bluetongue virus VP5 Open
The unenveloped Bluetongue virus capsid comprises several structural layers, the inner two comprising a core, which assembles before addition of the outer proteins, VP2 and VP5. Two symmetric trimers of VP5 fit like pegs into two distinct …
View article: Amino acid and viral binding by the high-affinity Cationic Amino acidTransporter 1 (CAT1) from Mus musculus
Amino acid and viral binding by the high-affinity Cationic Amino acidTransporter 1 (CAT1) from Mus musculus Open
Arginine, lysine, and ornithine are critical to protein structure and function, the urea cycle, and intracellular signaling. These cationic amino acids are imported by several membrane transporters, most notably the Cationic Amino acid Tra…
View article: Anti-SARS-CoV-2 antibody dynamics after primary vaccination with two-dose inactivated whole-virus vaccine, heterologous mRNA-1273 vaccine booster, and Omicron breakthrough infection in Indonesian health care workers
Anti-SARS-CoV-2 antibody dynamics after primary vaccination with two-dose inactivated whole-virus vaccine, heterologous mRNA-1273 vaccine booster, and Omicron breakthrough infection in Indonesian health care workers Open
Background Data on the dynamics and persistence of humoral immunity against SARS-CoV-2 after primary vaccination with two-dose inactivated vaccine (CoronaVac) are limited. This study evaluated the sequential effects of prior infection, het…
View article: The impact of exchanging the light and heavy chains on the structures of bovine ultralong antibodies
The impact of exchanging the light and heavy chains on the structures of bovine ultralong antibodies Open
The third complementary-determining regions of the heavy-chain (CDR3H) variable regions (VH) of some cattle antibodies are highly extended, consisting of 48 or more residues. These `ultralong' CDR3Hs form β-ribbon stalks that protrude from…