David Tezé
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View article: Crystal structure of the sucrose phosphorylase from <i>Alteromonas mediterranea</i> shows a loop transition in the active site
Crystal structure of the sucrose phosphorylase from <i>Alteromonas mediterranea</i> shows a loop transition in the active site Open
Sucrose phosphorylases are essential enzymes regulating sucrose metabolism, and it has been shown that a loop rearrangement is essential to their catalytic cycle. Crystal structures of only six sucrose phosphorylase enzymes are available. …
View article: A spontaneous proton transfer is key for enzymatic C-glycosylation and restricts the scope of natural C-glycosides
A spontaneous proton transfer is key for enzymatic C-glycosylation and restricts the scope of natural C-glycosides Open
C-glycosides are valuable compounds containing hydrolytically stable C-C bonds. However, their scarcity in nature and their complex synthesis limit their availability. Enzymes represent an environmentally mild paradigm for the synthesis of…
View article: GASP: A Pan-Specific Predictor of Family 1 Glycosyltransferase Acceptor Specificity Enabled by a Pipeline for Substrate Feature Generation and Large-Scale Experimental Screening
GASP: A Pan-Specific Predictor of Family 1 Glycosyltransferase Acceptor Specificity Enabled by a Pipeline for Substrate Feature Generation and Large-Scale Experimental Screening Open
Glycosylation represents a major chemical challenge; while it is one of the most common reactions in Nature, conventional chemistry struggles with stereochemistry, regioselectivity, and solubility issues. In contrast, family 1 glycosyltran…
View article: Regioselective Glycosylation of Polyphenols by Family 1 Glycosyltransferases: Experiments and Simulations
Regioselective Glycosylation of Polyphenols by Family 1 Glycosyltransferases: Experiments and Simulations Open
Family 1 glycosyltransferases (GT1s, UGTs) form natural product glycosides with exquisite control over regio- and stereoselectivity, representing attractive biotechnological targets. However, regioselectivity cannot be predicted and large-…
View article: GASP: A pan-specific predictor of family 1 glycosyltransferase specificity enabled by a pipeline for substrate feature generation and large-scale experimental screening
GASP: A pan-specific predictor of family 1 glycosyltransferase specificity enabled by a pipeline for substrate feature generation and large-scale experimental screening Open
Glycosylation represents a major chemical challenge; while it is one of the most common reactions in Nature, conventional chemistry struggles with stereochemistry, regioselectivity and solubility issues. In contrast, family 1 glycosyltrans…
View article: Regioselective glycosylation of polyphenols by family 1 glycosyltransferases: experiments and simulations
Regioselective glycosylation of polyphenols by family 1 glycosyltransferases: experiments and simulations Open
Family 1 glycosyltransferases (GT1s, UGTs) form natural product glycosides with exquisite control over regio- and stereoselectivity, representing attractive biotechnological targets. However, regioselectivity cannot be predicted and large-…
View article: The Engineered Hexosaminidase <i>Tt</i> OGA-D120N Is an Efficient <i>O</i> -/ <i>N</i> -/ <i>S</i> -Glycoligase That Also Catalyzes Formation and Release of Oxazoline Donors for Cascade Syntheses with Glycosynthases or Transglycosylases
The Engineered Hexosaminidase <i>Tt</i> OGA-D120N Is an Efficient <i>O</i> -/ <i>N</i> -/ <i>S</i> -Glycoligase That Also Catalyzes Formation and Release of Oxazoline Donors for Cascade Syntheses with Glycosynthases or Transglycosylases Open
Engineering glycoside hydrolases is a major route to obtaining catalysts forming glycosidic bonds. Glycosynthases, thioglycoligases, and transglycosylases represent the main strategies, each having advantages and drawbacks. Here, we show t…
View article: Promiscuous yet specific: a methionine-aromatics interaction drives the reaction scope of the family 1 glycosyltransferase GmUGT88E3 from soybean.
Promiscuous yet specific: a methionine-aromatics interaction drives the reaction scope of the family 1 glycosyltransferase GmUGT88E3 from soybean. Open
Family 1 glycosyltransferases (GT1s, UGTs) catalyze the regioselective glycosylation of natural products in a single step. We identified GmUGT88E3 as a particularly promising biocatalyst, able to produce a variety of pure, single glycosidi…
View article: The engineered hexosaminidase TtOGA-D120N is an efficient O-/N-/S-glycoligase that also catalyzes formation and release of oxazoline donors for cascade syntheses with glycosynthases or transglycosylases
The engineered hexosaminidase TtOGA-D120N is an efficient O-/N-/S-glycoligase that also catalyzes formation and release of oxazoline donors for cascade syntheses with glycosynthases or transglycosylases Open
Engineering glycoside hydrolases is a major route to obtain catalysts forming glycosidic bonds. Glycosynthases, thioglycoligases and transglycosylases represent the main strategies, each having advantages and drawbacks. Here, we show that …
View article: Sucrose phosphorylase from<i>Alteromonas mediterranea</i>: structural insight into the regioselective α-glucosylation of (+)-catechin
Sucrose phosphorylase from<i>Alteromonas mediterranea</i>: structural insight into the regioselective α-glucosylation of (+)-catechin Open
Sucrose phosphorylases, through transglycosylation reactions, are interesting enzymes that can transfer regioselectively glucose from sucrose, the donor substrate, onto acceptors like flavonoids to form glycoconjugates and hence modulate t…
View article: Sequence mining yields 18 phloretin C‐glycosyltransferases from plants for the efficient biocatalytic synthesis of nothofagin and phloretin‐di‐C‐glycoside
Sequence mining yields 18 phloretin C‐glycosyltransferases from plants for the efficient biocatalytic synthesis of nothofagin and phloretin‐di‐C‐glycoside Open
C ‐glycosyltransferases ( C ‐GTs) offer selective and efficient synthesis of natural product C ‐glycosides under mild reaction conditions. In contrast, the chemical synthesis of these C ‐glycosides is challenging and environmentally harmfu…
View article: Chemoenzymatic indican for sustainable light-driven denim dyeing
Chemoenzymatic indican for sustainable light-driven denim dyeing Open
Blue denim, a billion-dollar industry, is currently dyed with indigo in an unsustainable process requiring harsh reducing and alkaline chemicals. Several innovations aim to replace the damaging chemicals and processes with ecologically att…
View article: 1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii
1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii Open
To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy…
View article: Family 1 glycosyltransferases (GT1, UGTs) are subject to dilution-induced inactivation and low chemo stability toward their own acceptor substrates
Family 1 glycosyltransferases (GT1, UGTs) are subject to dilution-induced inactivation and low chemo stability toward their own acceptor substrates Open
Glycosylation reactions are essential but challenging from a conventional chemistry standpoint. Conversely, they are biotechnologically feasible as glycosyltransferases can transfer sugar to an acceptor with perfect regio- and stereo-selec…
View article: Family 1 Glycosyltransferase UGT706F8 from <i>Zea mays</i> Selectively Catalyzes the Synthesis of Silibinin 7-<i>O</i>-β-<scp>d</scp>-Glucoside
Family 1 Glycosyltransferase UGT706F8 from <i>Zea mays</i> Selectively Catalyzes the Synthesis of Silibinin 7-<i>O</i>-β-<span>d</span>-Glucoside Open
Regioselective glycosylation is a chemical challenge, leading to multistep syntheses with protecting group manipulations, ultimately resulting in poor atom economy and compromised sustainability. Enzymes allow eco-friendly and regioselecti…
View article: Family 1 glycosyltransferases (GT1, UGTs) are subject to dilution-induced inactivation and low chemo stability towards their own acceptor substrates
Family 1 glycosyltransferases (GT1, UGTs) are subject to dilution-induced inactivation and low chemo stability towards their own acceptor substrates Open
Glycosylation reactions are essential but challenging from a conventional chemistry standpoint. Conversely, they are biotechnologically feasible as glycosyltransferases can transfer a sugar to an acceptor with perfect regio- and stereo- se…
View article: Oligomerization engineering of the fluorinase enzyme leads to an active trimer that supports synthesis of fluorometabolites <i>in vitro</i>
Oligomerization engineering of the fluorinase enzyme leads to an active trimer that supports synthesis of fluorometabolites <i>in vitro</i> Open
Summary The fluorinase enzyme represents the only biological mechanism capable of forming stable C–F bonds characterized in nature thus far, offering a biotechnological route to the biosynthesis of value‐added organofluorines. The fluorina…
View article: Computer Simulation to Rationalize “Rational” Engineering of Glycoside Hydrolases and Glycosyltransferases
Computer Simulation to Rationalize “Rational” Engineering of Glycoside Hydrolases and Glycosyltransferases Open
Glycoside hydrolases and glycosyltransferases are the main classes of enzymes that synthesize and degrade carbohydrates, molecules essential to life that are a challenge for classical chemistry. As such, considerable efforts have been made…
View article: Characterization of five marine family 29 glycoside hydrolases reveals an α-L-fucosidase targeting specifically Fuc( <i>α</i> 1,4)GlcNAc
Characterization of five marine family 29 glycoside hydrolases reveals an α-L-fucosidase targeting specifically Fuc( <i>α</i> 1,4)GlcNAc Open
$\text{L} $ -Fucose is the most widely distributed $\text{L} $-hexose in marine and terrestrial environments and presents a variety of functional roles. $\text{L} $-Fucose is the major monosaccharide in the polysaccharide fucoidan from cel…
View article: Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7
Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7 Open
Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass. In this respect, alginate epimerases and lyases are valuable enzymes for tailoring…
View article: A healthy Bifidobacterium dentium caramel cocktail
A healthy Bifidobacterium dentium caramel cocktail Open
β-d-fructofuranosyl glycosidases are enzymes that produce health-beneficial fructooligosaccharides from natural fructans. In a recent issue of JBC, Kashima et al. identified a novel α-d-fructofuranosyl-active enzyme, αFFase1, from the cari…
View article: Exploring the in Vitro Operating Window of Glycosyltransferase PtUGT1 from Polygonum tinctorium for a Biocatalytic Route to Indigo Dye
Exploring the in Vitro Operating Window of Glycosyltransferase PtUGT1 from Polygonum tinctorium for a Biocatalytic Route to Indigo Dye Open
The eobiotic compound indican lends itself to a compelling biocatalytic dyeing strategy for denim, in which the formation of corrosive byproducts is avoided. However, the efficient and scalable production of indican remains a key bottlenec…
View article: Exploring the in Vitro Operating Window of Glycosyltransferase PtUGT1 from Polygonum tinctorium for a Biocatalytic Route to Indigo Dye
Exploring the in Vitro Operating Window of Glycosyltransferase PtUGT1 from Polygonum tinctorium for a Biocatalytic Route to Indigo Dye Open
The eobiotic compound indican lends itself to a compelling biocatalytic dyeing strategy for denim, in which the formation of corrosive byproducts is avoided. However, the efficient and scalable production of indican remains a key bottlenec…
View article: Exploring the <i>in Vitro</i> Operating Window of Glycosyltransferase <i>Pt</i> UGT1 from <i>Polygonum tinctorium</i> for a Biocatalytic Route to Indigo Dye
Exploring the <i>in Vitro</i> Operating Window of Glycosyltransferase <i>Pt</i> UGT1 from <i>Polygonum tinctorium</i> for a Biocatalytic Route to Indigo Dye Open
The eobiotic compound indican lends itself to a compelling biocatalytic dyeing strategy for denim, in which the formation of corrosive byproducts is avoided. However, the efficient and scalable production of indican remains a key bottlenec…
View article: Two marine GH29 <i>α</i>-L-fucosidases from an uncultured <i>Paraglaciecola</i> sp. specifically hydrolyze fucosyl-<i>N</i>-acetylglucosamine regioisomers
Two marine GH29 <i>α</i>-L-fucosidases from an uncultured <i>Paraglaciecola</i> sp. specifically hydrolyze fucosyl-<i>N</i>-acetylglucosamine regioisomers Open
l -Fucose is the most widely distributed l -hexose in marine and terrestrial environments, and presents a variety of functional roles. l -Fucose is the major monosaccharide in the polysaccharide fucoidan from cell walls of brown algae, and…
View article: Rational Enzyme Design without Structural Knowledge: A Sequence‐Based Approach for Efficient Generation of Transglycosylases
Rational Enzyme Design without Structural Knowledge: A Sequence‐Based Approach for Efficient Generation of Transglycosylases Open
Glycobiology is dogged by the relative scarcity of synthetic, defined oligosaccharides. Enzyme‐catalysed glycosylation using glycoside hydrolases is feasible but is hampered by the innate hydrolytic activity of these enzymes. Protein engin…