Mark D. Tully
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Toward Nanodisc Tailoring for SANS Study of Membrane Proteins Open
Although membrane proteins are of major importance in both physiology and disease, they remain less studied than soluble proteins due to the complex amphiphilic environments required to preserve their structure and function. As a consequen…
Transcriptional outputs and condensates – formation and function Open
Summary The dynamic spatiotemporal organization of macromolecules is key to the proper function of the cell, allowing the exquisite regulation of diverse processes from gene expression to enzymatic function. The formation of biomolecular c…
HP1 loses its chromatin clustering and phase separation function across evolution Open
Heterochromatin protein 1 (HP1) is a multifunctional chromatin-associated protein conserved from fission yeast to mammals. HP1 has been suggested to drive heterochromatin formation via phase separation. However, there is seemingly conflict…
A Versatile Sample Chamber for BioSAXS: Integration and Applications on the P12 Beamline Open
We present the integration of a sample chamber for advanced BioSAXS measurement on the P12 beamline of EMBL Hamburg. The sample chamber is designed to be installed in the beamline P12 at PETRA and BM29 at ESRF with minimal effort to swap t…
View article: Current and future perspectives for structural biology at the Grenoble EPN campus: a comprehensive overview
Current and future perspectives for structural biology at the Grenoble EPN campus: a comprehensive overview Open
The European Photon and Neutron campus in Grenoble is a unique site, encompassing the European Synchrotron Radiation Facility Extremely Brilliant Source, the Institut Laue–Langevin, the European Molecular Biology Laboratory and the Institu…
Structure–function mapping and mechanistic insights on the <span>SARS CoV2</span> Nsp1 Open
Non‐structural protein 1 (Nsp1) is a key component of the infectious process caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV2), responsible for the COVID‐19 pandemic. Our previous data demonstrated that Nsp1 can deg…
Linkage-specific ubiquitin binding interfaces modulate the activity of the chlamydial deubiquitinase Cdu1 towards poly-ubiquitin substrates Open
The chlamydial deubiquitinase Cdu1 of the obligate intracellular human pathogenic bacterium Chlamydia trachomatis plays important roles in the maintenance of chlamydial infection. Despite the structural similarities shared with its homolog…
Enhanced structure/function of mTSPO translocator in lipid:surfactant mixed micelles Open
TSPO is a ubiquitous transmembrane protein used as a pharmacological marker in neuroimaging. The only known atomic structure of mammalian TSPOs comes from the solution NMR of mouse TSPO (mTSPO) bound to the PK11195 ligand and in a DPC surf…
SARS-CoV2 Nsp1 is a metal-dependent DNA and RNA endonuclease Open
Over recent years, we have been living under a pandemic, caused by the rapid spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV2). One of the major virulence factors of Coronaviruses is the Non-structural protein 1 (Ns…
The structural properties of full-length annexin A11 Open
Annexin A11 (ANXA11) is a calcium-dependent phospholipid-binding protein belonging to the annexin protein family and implicated in the neurodegenerative amyotrophic lateral sclerosis. Structurally, ANXA11 contains a conserved calcium-bindi…
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Open
The mitogen-activated protein kinase (MAPK) p38α is a central component of signaling in inflammation and the immune response and is, therefore, an important drug target. Little is known about the molecular mechanism of its activation by do…
Phase separation and molecular ordering of the prion-like domain of the Arabidopsis thermosensory protein EARLY FLOWERING 3 Open
Liquid–liquid phase separation (LLPS) is an important mechanism enabling the dynamic compartmentalization of macromolecules, including complex polymers such as proteins and nucleic acids, and occurs as a function of the physicochemical env…
Structural insights into the bi-specific cross-over dual variable antibody architecture by cryo-EM Open
Multi-specific antibodies (msAbs) are being developed as next generation antibody-based therapeutics. Knowledge of the three-dimensional structures, in the full antibody context, of their fragment antigen-binding (Fab) moieties with or wit…
Interaction of a Histidine-Rich Antimicrobial Saliva Peptide with Model Cell Membranes: The Role of Histidines Open
Histatin 5 is a histidine-rich, intrinsically disordered, multifunctional saliva protein known to act as a first line of defense against oral candidiasis caused by Candida albicans. An earlier study showed that, upon interaction with a com…
Phase separation and molecular ordering of the prion-like domain of the thermosensory protein EARLY FLOWERING 3 Open
Liquid-liquid phase separation (LLPS) is an important mechanism enabling the dynamic compartmentalisation of macromolecules, including complex polymers such as proteins and nucleic acids, and occurs as a function of the physicochemical env…
BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software Open
As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF's BM29 BioSAXS beamline was subject to a significant upgrade and refurbishment. In addition to the replacement of the beamline's original bending magnet source by a …
New data analysis for BioSAXS at the ESRF Open
The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a ne…
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Open
The MAP kinase p38α is a central component of signalling in inflammation and the immune response and is, therefore, an important drug target. Little is known about the molecular mechanism of its activation by double-phosphorylation from MA…
Impact of Arginine–Phosphate Interactions on the Reentrant Condensation of Disordered Proteins Open
Re-entrant condensation results in the formation of a condensed protein regime between two critical ion concentrations. The process is driven by neutralization and inversion of the protein charge by oppositely charged ions. Re-entrant cond…
Analysis of SEC-SAXS data via EFA deconvolution and Scatter Open
BioSAXS is a popular technique used in molecular and structural biology to determine the solution structure, particle size and shape, surface-to-volume ratio and conformational changes of macromolecules and macromolecular complexes. A high…
Benzene tricarboxamide derivatives with lipid and ethylene glycol chains self-assemble into distinct nanostructures driven by molecular packing Open
The self-assembly in aqueous solution of benzene-1,3,5-tricarboxamide (BTA) bearing one alkyl chain and two PEG (polyethylene glycol) chains or two alkyl chains and one PEG chain yields molecular-packing driven nanostructures.
Beamline B21: high-throughput small-angle X-ray scattering at Diamond Light Source Open
B21 is a small-angle X-ray scattering (SAXS) beamline with a bending magnet source in the 3 GeV storage ring at the Diamond Light Source Ltd synchrotron in the UK. The beamline utilizes a double multi-layer monochromator and a toroidal foc…
From MXCuBE3 to BSXCuBE3 a Web Application for BioSAXS Experiment Control Open
A new version of the beamline control application BSXCuBE (BioSAXS Customized Beamline Environment) designed to control BioSAXS experiments at the new ESRF Extremely Brilliant Source (EBS) is under development. The new application is imple…
View article: KAP1 is an antiparallel dimer with a functional asymmetry
KAP1 is an antiparallel dimer with a functional asymmetry Open
KAP1 (KRAB domain–associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In doing so, KAP1 acts both as a platform for…