David W. Dorward
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View article: Scanning Electron Microscopy
Scanning Electron Microscopy Open
Scanning electron microscopy (SEM) remains distinct in its ability to allow topographical visualization of structures. Key elements to consider for successful examination of biological specimens include appropriate preparative and imaging …
View article: 1305 Agreement and reliability of an automated PD-L1 tumor proportion scoring algorithm in non-small cell lung cancer (NSCLC)
1305 Agreement and reliability of an automated PD-L1 tumor proportion scoring algorithm in non-small cell lung cancer (NSCLC) Open
Background Immunotherapy has revolutionized the treatment paradigm of advanced non-small cell lung cancer (NSCLC), opening possibilities for long-term survival outcomes with superior tolerability.1 Eligibility for immune checkpo…
View article: Ebola virus shed glycoprotein is toxic to human T, B, and natural killer lymphocytes
Ebola virus shed glycoprotein is toxic to human T, B, and natural killer lymphocytes Open
Lymphocyte depletion is a distinctive feature of Ebola virus (EBOV) disease. The ectodomain of EBOV glycoprotein (GP) is cleaved off the surface of infected cells into circulation as shed GP. To test the hypothesis that shed GP induces lym…
View article: Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete
Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete Open
The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi . We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bb…
View article: Intranasal Murine Pneumonia Virus-Vectored SARS-CoV-2 Vaccine Induces Mucosal and Serum Antibodies in Macaques
Intranasal Murine Pneumonia Virus-Vectored SARS-CoV-2 Vaccine Induces Mucosal and Serum Antibodies in Macaques Open
View article: Intranasal immunization with avian paramyxovirus type 3 expressing SARS-CoV-2 spike protein protects hamsters against SARS-CoV-2
Intranasal immunization with avian paramyxovirus type 3 expressing SARS-CoV-2 spike protein protects hamsters against SARS-CoV-2 Open
View article: Brugia malayi Microfilariae Induce Autophagy through an Interferon-γ Dependent Mechanism in Human Monocytes
Brugia malayi Microfilariae Induce Autophagy through an Interferon-γ Dependent Mechanism in Human Monocytes Open
Monocyte dysfunction in helminth infection is one of the mechanisms proposed to explain the diminished parasite antigen-specific T cell responses seen with patent filarial infection. In fact, monocytes from filariae-infected individuals de…
View article: A Chlamydial Plasmid-Dependent Secretion System for the Delivery of Virulence Factors to the Host Cytosol
A Chlamydial Plasmid-Dependent Secretion System for the Delivery of Virulence Factors to the Host Cytosol Open
The Chlamydia trachomatis plasmid regulates the expression and secretion of immune evasion virulence factors to the host cytosol by an unknown mechanism. In this study, we identified a novel plasmid gene protein 4 (Pgp4)-dependent secretio…
View article: Extracellular vesicles released from the filarial parasite Brugia malayi downregulate the host mTOR pathway
Extracellular vesicles released from the filarial parasite Brugia malayi downregulate the host mTOR pathway Open
We have previously shown that the microfilarial (mf) stage of Brugia malayi can inhibit the mammalian target of rapamycin (mTOR; a conserved serine/threonine kinase critical for immune regulation and cellular growth) in human dendritic cel…
View article: The liver X receptor agonist LXR 623 restricts flavivirus replication
The liver X receptor agonist LXR 623 restricts flavivirus replication Open
The vector-borne flaviviruses (VBFVs) are well known for causing great misery and death in humans worldwide. The VBFVs include those transmitted by mosquitos, such as Zika virus (ZIKV), dengue virus; and those transmitted by ticks includin…
View article: <i>Trans</i>-endocytosis of intact IL-15Rα–IL-15 complex from presenting cells into NK cells favors signaling for proliferation
<i>Trans</i>-endocytosis of intact IL-15Rα–IL-15 complex from presenting cells into NK cells favors signaling for proliferation Open
Significance Interleukin 15 (IL-15) is essential for natural killer (NK) cell survival and proliferation. The IL-15 receptor consists of 3 subunits: the α chain and the β and common γ (γ c ) chains, which are shared with the IL-2 receptor.…
View article: A single nucleotide polymorphism in the Plasmodium falciparum atg18 gene associates with artemisinin resistance and confers enhanced parasite survival under nutrient deprivation
A single nucleotide polymorphism in the Plasmodium falciparum atg18 gene associates with artemisinin resistance and confers enhanced parasite survival under nutrient deprivation Open
View article: Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells
Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells Open
Early secretion of IL-12 by mouse dendritic cells (DCs) instructs T cells to make IFN-γ. However, only activated, but not naive T cells are able to license DCs for IL-12 production. We hypothesized that it might be due to different levels …
View article: Ultrastructure and pathology of prion protein amyloid accumulation and cellular damage in extraneural tissues of scrapie-infected transgenic mice expressing anchorless prion protein
Ultrastructure and pathology of prion protein amyloid accumulation and cellular damage in extraneural tissues of scrapie-infected transgenic mice expressing anchorless prion protein Open
In most human and animal prion diseases the abnormal disease-associated prion protein (PrPSc) is deposited as non-amyloid aggregates in CNS, spleen and lymphoid organs. In contrast, in humans and transgenic mice with PrP mutations which ca…
View article: Early Intracellular Trafficking of Granulibacter bethesdensis in Human Macrophages
Early Intracellular Trafficking of Granulibacter bethesdensis in Human Macrophages Open
Granulibacter bethesdensis is a Gram-negative bacterium that infects patients with chronic granulomatous disease (CGD), a primary immunodeficiency marked by a defect in NOX2, the phagocyte NADPH oxidase. Previous studies have shown that NO…
View article: Cellular prion protein is present in mitochondria of healthy mice
Cellular prion protein is present in mitochondria of healthy mice Open
View article: Cytoarchitecture of Zika virus infection in human neuroblastoma and Aedes albopictus cell lines
Cytoarchitecture of Zika virus infection in human neuroblastoma and Aedes albopictus cell lines Open
The Zika virus (ZIKV) pandemic is a global concern due to its role in the development of congenital anomalies of the central nervous system. This mosquito-borne flavivirus alternates between mammalian and mosquito hosts, but information ab…
View article: Packaging and Prefusion Stabilization Separately and Additively Increase the Quantity and Quality of Respiratory Syncytial Virus (RSV)-Neutralizing Antibodies Induced by an RSV Fusion Protein Expressed by a Parainfluenza Virus Vector
Packaging and Prefusion Stabilization Separately and Additively Increase the Quantity and Quality of Respiratory Syncytial Virus (RSV)-Neutralizing Antibodies Induced by an RSV Fusion Protein Expressed by a Parainfluenza Virus Vector Open
Human respiratory syncytial virus (RSV) and human parainfluenza virus type 3 (HPIV3) are major pediatric respiratory pathogens that lack vaccines. A chimeric bovine/human PIV3 (rB/HPIV3) virus expressing the unmodified, wild-type (wt) RSV …
View article: Zinc-Induced Polymerization of Killer-Cell Ig-like Receptor into Filaments Promotes Its Inhibitory Function at Cytotoxic Immunological Synapses
Zinc-Induced Polymerization of Killer-Cell Ig-like Receptor into Filaments Promotes Its Inhibitory Function at Cytotoxic Immunological Synapses Open
View article: Phagocytosis and Killing of Carbapenem-Resistant ST258<i>Klebsiella pneumoniae</i>by Human Neutrophils
Phagocytosis and Killing of Carbapenem-Resistant ST258<i>Klebsiella pneumoniae</i>by Human Neutrophils Open
Carbapenem-resistant Klebsiella pneumoniae strains classified as multilocus sequence type 258 (ST258) are among the most widespread multidrug-resistant hospital-acquired pathogens. Treatment of infections caused by these organisms is diffi…
View article: Detection of PrPres by immunoblot using monoclonal antibody D13.
Detection of PrPres by immunoblot using monoclonal antibody D13. Open
Comparison of PrPres in brain of 22L scrapie-infected tg44+/+ and tg44+/− mice at the time of clinical disease. All lanes were loaded with 0.25 mg brain tissue equivalents. A clinical Prnp+/− mouse is shown for comparison. Lane 1: Prnp+/− …
View article: Author Index
Author Index Open
View article: Prion Protein Prolines 102 and 105 and the Surrounding Lysine Cluster Impede Amyloid Formation
Prion Protein Prolines 102 and 105 and the Surrounding Lysine Cluster Impede Amyloid Formation Open
View article: Efficient Uptake and Dissemination of Scrapie Prion Protein by Astrocytes and Fibroblasts from Adult Hamster Brain
Efficient Uptake and Dissemination of Scrapie Prion Protein by Astrocytes and Fibroblasts from Adult Hamster Brain Open
Prion infections target neurons and lead to neuronal loss. However, the role of non-neuronal cells in the initiation and spread of infection throughout the brain remains unclear despite the fact these cells can also propagate prion infecti…
View article: Validation of Normal Human Bronchial Epithelial Cells as a Model for Influenza A Infections in Human Distal Trachea
Validation of Normal Human Bronchial Epithelial Cells as a Model for Influenza A Infections in Human Distal Trachea Open
Primary normal human bronchial/tracheal epithelial (NHBE) cells, derived from the distal-most aspect of the trachea at the bifurcation, have been used for a number of studies in respiratory disease research. Differences between the source …
View article: Author Index
Author Index Open