David S. Weiss
YOU?
Author Swipe
View article: Defining the Temporal Progression of Normal and Nonunion Human Humeral Shaft Using Plasma Proteome
Defining the Temporal Progression of Normal and Nonunion Human Humeral Shaft Using Plasma Proteome Open
Current diagnostic tools are neither informative of, nor concurrent with the underlying biological processes of fracture healing. We examined if there would be protein markers in the plasma of non-operatively fixed, isolated, non-articular…
View article: Analysis of essential genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq
Analysis of essential genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq Open
Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in Clostridioides difficile , a problematic pathogen for which treatment…
View article: FtsZ-mediated spatial–temporal control over septal cell wall synthesis
FtsZ-mediated spatial–temporal control over septal cell wall synthesis Open
FtsZ, the tubulin-like GTPase, is the central organizer of the bacterial divisome, a macromolecular complex that synthesizes new septal cell wall (made of septal peptidoglycan, sPG) to allow cell wall constriction and cytokinesis. In Esche…
View article: Analysis of Essential Genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq
Analysis of Essential Genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq Open
Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in Clostridioides difficile , a problematic pathogen for which treatment…
View article: 92 Prevalence of heteroresistance in urinary <i>Escherichia coli</i> in Metropolitan Atlanta, Georgia
92 Prevalence of heteroresistance in urinary <i>Escherichia coli</i> in Metropolitan Atlanta, Georgia Open
Objectives/Goals: Urinary tract infections (UTIs) cause significant morbidity, and many patients require multiple courses of antibiotics increasing the risk of antibiotic resistance. We determined the prevalence of urinary antibiotic heter…
View article: The association between undetected heteroresistance and antibiotic treatment failure in complicated urinary tract infection
The association between undetected heteroresistance and antibiotic treatment failure in complicated urinary tract infection Open
Background Antibiotic resistance is a worsening public health threat. One poorly understood aspect of this problem is unexpected antibiotic treatment failure; when an infecting isolate is deemed susceptible to a given antibiotic, yet treat…
View article: Identification of two glycosyltransferases required for synthesis of membrane glycolipids in <i>Clostridioides difficile</i>
Identification of two glycosyltransferases required for synthesis of membrane glycolipids in <i>Clostridioides difficile</i> Open
Clostridioides difficile infections cause over 12,000 deaths and an estimated one billion dollars in healthcare costs annually in the United States. The cell membrane is an essential structure that is important for protection from the extr…
View article: Identification of two glycosyltransferases required for synthesis of membrane glycolipids in <i>Clostridioides difficile</i>
Identification of two glycosyltransferases required for synthesis of membrane glycolipids in <i>Clostridioides difficile</i> Open
Clostridioides difficile infections cause over 12,000 deaths and an estimated one billion dollars in healthcare costs annually in the United States. The cell membrane is an essential structure that is important for protection from the extr…
View article: The phospholipase A of <i>Neisseria gonorrhoeae</i> lyses eukaryotic membranes and is necessary for survival in neutrophils and cervical epithelial cells
The phospholipase A of <i>Neisseria gonorrhoeae</i> lyses eukaryotic membranes and is necessary for survival in neutrophils and cervical epithelial cells Open
Contact-dependent hemolysins are virulence factors in a number of human pathogens, including Helicobacter pylori, Rickettsia typhi, Bartonella bacilliformis, Mycobacterium tuberculosis, entero-invasive Escherichia coli, and Shigella . Here…
View article: Identification of a family of peptidoglycan transpeptidases reveals that <i>Clostridioides difficile</i> requires noncanonical cross-links for viability
Identification of a family of peptidoglycan transpeptidases reveals that <i>Clostridioides difficile</i> requires noncanonical cross-links for viability Open
Most bacteria are surrounded by a cell wall that contains peptidoglycan (PG), a large polymer composed of glycan strands held together by short peptide cross-links. There are two major types of cross-links, termed 4-3 and 3-3 based on the …
View article: <i>E. coli</i>FtsN coordinates synthesis and degradation of septal peptidoglycan by partitioning between a synthesis track and a denuded glycan track
<i>E. coli</i>FtsN coordinates synthesis and degradation of septal peptidoglycan by partitioning between a synthesis track and a denuded glycan track Open
The E. coli cell division protein FtsN was proposed to coordinate septal peptidoglycan (sPG) synthesis and degradation to ensure robust cell wall constriction without lethal lesions. Although the precise mechanism remains unclear, previous…
View article: Identification of a new family of peptidoglycan transpeptidases reveals atypical crosslinking is essential for viability in<i>Clostridioides difficile</i>
Identification of a new family of peptidoglycan transpeptidases reveals atypical crosslinking is essential for viability in<i>Clostridioides difficile</i> Open
Clostridioides difficile , the leading cause of antibiotic-associated diarrhea, relies primarily on 3-3 crosslinks created by L,D-transpeptidases (LDTs) to fortify its peptidoglycan (PG) cell wall. This is unusual, as in most bacteria the …
View article: Identification of <i>Clostridioides difficile</i> mutants with increased daptomycin resistance
Identification of <i>Clostridioides difficile</i> mutants with increased daptomycin resistance Open
Daptomycin is a cyclic lipopeptide antibiotic used to treat infections caused by some Gram-positive bacteria. Daptomycin disrupts synthesis of the peptidoglycan (PG) cell wall by inserting into the cytoplasmic membrane and binding multiple…
View article: Dissociation kinetics of small-molecule inhibitors in Escherichia coli is coupled to physiological state of cells
Dissociation kinetics of small-molecule inhibitors in Escherichia coli is coupled to physiological state of cells Open
Bioactive small-molecule inhibitors represent a treasure chest for future drugs. In vitro high-throughput screening is a common approach to identify the small-molecule inhibitors that bind tightly to purified targets. Here, we investigate …
View article: FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli
FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli Open
FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG) by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here we use single-molecule tracking to investigate Fts…
View article: Comparative Study of Bacterial SPOR Domains Identifies Functionally Important Differences in Glycan Binding Affinity
Comparative Study of Bacterial SPOR Domains Identifies Functionally Important Differences in Glycan Binding Affinity Open
SPOR domain proteins are prominent components of the cell division apparatus in a wide variety of bacteria. The primary function of SPOR domains is targeting proteins to the division site, which they accomplish by binding to septal peptido…
View article: Comparative study of bacterial SPOR domains identifies functionally important differences in glycan binding affinity
Comparative study of bacterial SPOR domains identifies functionally important differences in glycan binding affinity Open
Bacterial SPOR domains target proteins to the divisome by binding septal peptidoglycan (PG) at sites where cell wall amidases have removed stem peptides. These PG structures are referred to as denuded glycans. Although all characterized SP…
View article: The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile
The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile Open
The WalRK two-component system (TCS) is essential for coordinating synthesis and turnover of peptidoglycan in Firmicutes . We investigated the WalRK TCS in Clostridioides difficile , an important bacterial pathogen with an atypical cell en…
View article: The WalRK two-component system is essential for proper cell envelope biogenesis in <i>Clostridioides difficile</i>
The WalRK two-component system is essential for proper cell envelope biogenesis in <i>Clostridioides difficile</i> Open
The WalR-WalK two-component regulatory system (TCS) is found in all Firmicutes, where it regulates the expression of multiple genes required for remodeling the cell envelope during growth and division. Unlike most TCSs, WalRK is essential …
View article: FtsN activates septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthase in <i>E. coli</i>
FtsN activates septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthase in <i>E. coli</i> Open
The FtsN protein of Escherichia coli and other proteobacteria is an essential and highly conserved bitopic membrane protein that triggers the inward synthesis of septal peptidoglycan (sPG) during cell division. Previous work has shown that…
View article: Faculty Opinions recommendation of Essential role for ftsl in activation of septal peptidoglycan synthesis.
Faculty Opinions recommendation of Essential role for ftsl in activation of septal peptidoglycan synthesis. Open
Spatiotemporal regulation of septal peptidoglycan (PG) synthesis is achieved by coupling assembly and activation of the synthetic enzymes (FtsWI) to the Z ring, a cytoskeletal element that is required for division in most bacteria. In Esch…
View article: Micafungin and amphotericin B synergy against Candida auris
Micafungin and amphotericin B synergy against Candida auris Open
View article: DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli
DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli Open
A thorough understanding of bacterial cell division requires identifying and characterizing all of the proteins that participate in this process. Our discovery of DrpB brings us one step closer to this goal in E. coli .
View article: Faculty Opinions recommendation of Structural Coordination of Polymerization and Crosslinking by a SEDS-bPBP Peptidoglycan Synthase Complex.
Faculty Opinions recommendation of Structural Coordination of Polymerization and Crosslinking by a SEDS-bPBP Peptidoglycan Synthase Complex. Open
The Shape, Elongation, Division, and Sporulation ("SEDS") proteins are a highly conserved family of transmembrane glycosyltransferases that work in concert with class B penicillin binding proteins (bPBPs) to build the bacterial peptidoglyc…
View article: Nitric oxide disrupts bacterial cytokinesis by poisoning purine metabolism
Nitric oxide disrupts bacterial cytokinesis by poisoning purine metabolism Open
An inflammatory mediator interrupts early steps in bacterial cell division by inhibiting nucleoside triphosphate synthesis.
View article: Combinations of antifungals to effectively kill drug-resistant <i>Candida auris</i>
Combinations of antifungals to effectively kill drug-resistant <i>Candida auris</i> Open
Candida auris is an emerging, highly virulent fungal pathogen that is often resistant to multiple classes of antifungals. To overcome this resistance, we investigated the in vitro efficacy of antifungal combinations, finding that amphoteri…
View article: Faculty Opinions recommendation of A switch in surface polymer biogenesis triggers growth-phase-dependent and antibiotic-induced bacteriolysis.
Faculty Opinions recommendation of A switch in surface polymer biogenesis triggers growth-phase-dependent and antibiotic-induced bacteriolysis. Open
Penicillin and related antibiotics disrupt cell wall synthesis to induce bacteriolysis.Lysis in response to these drugs requires the activity of cell wall hydrolases called autolysins, but how penicillins misactivate these deadly enzymes h…
View article: A Xylose-Inducible Expression System and a CRISPR Interference Plasmid for Targeted Knockdown of Gene Expression in Clostridioides difficile
A Xylose-Inducible Expression System and a CRISPR Interference Plasmid for Targeted Knockdown of Gene Expression in Clostridioides difficile Open
Clostridioides difficile has become the leading cause of hospital-acquired diarrhea in developed countries. A better understanding of the basic biology of this devastating pathogen might lead to novel approaches for preventing or treating …
View article: Faculty Opinions recommendation of Regulation of the peptidoglycan polymerase activity of pbp1b by antagonist actions of the core divisome proteins ftsblq and ftsn.
Faculty Opinions recommendation of Regulation of the peptidoglycan polymerase activity of pbp1b by antagonist actions of the core divisome proteins ftsblq and ftsn. Open
Peptidoglycan (PG) is an essential constituent of the bacterial cell wall.During cell division, PG synthesis localizes at midcell under the control of a multiprotein complex, the divisome, allowing the safe formation of two new cell poles …
View article: Ftsn Bridges the FtsZ-Treadmilling and Septal Peptidoglycan Synthesis
Ftsn Bridges the FtsZ-Treadmilling and Septal Peptidoglycan Synthesis Open