David Wang
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Blockade of interleukin-6 (IL-6) signaling in dedifferentiated liposarcoma (DDLPS) decreases mouse double minute 2 (MDM2) oncogenicity via alternative splicing Open
Effective therapies for retroperitoneal (RP) dedifferentiated liposarcoma (DDLPS) remain unavailable. Loco-regional recurrence occurs in >80% of cases; 5-year disease-specific survival is only 20%. DDLPS is especially prevalent in the retr…
Orsay virus variants isolated from wild <i>Caenorhabditis elegans</i> nematodes, France Open
Four new variants of Orsay virus were identified from wild isolates of Caenorhabditis elegans nematodes collected from decaying plant matter in France. Near-complete genomes of the viruses were determined by metagenomic sequencing. The fou…
An SRR1 domain-containing protein is required for efficient Orsay virus replication in <i>Caenorhabditis elegans</i> Open
Viruses depend on their hosts for completing their life cycle, and a better understanding of virus replication can inform therapeutic strategies. Using the Orsay virus- Caenorhabditis elegans experimental platform, we identified by a forwa…
View article: Importance of outbreak response research in bridging knowledge gaps on emerging infectious diseases
Importance of outbreak response research in bridging knowledge gaps on emerging infectious diseases Open
An important outcome of the devastating 2014 West African Ebola virus disease outbreak and the 2020 COVID-19 pandemic has been the growing promotion of conducting research during outbreaks of emerging infectious diseases (EIDs) as a valuab…
Novel murine model of human astrovirus infection reveals cardiovascular tropism <b> </b> Open
Astroviruses are a common cause of gastrointestinal disease in humans and have been linked to fatal cases of encephalitis. A major barrier to the study of human-infecting astroviruses is the lack of an in vivo model as previous attempts fa…
View article: Barcoded SARS-CoV-2 viruses define the impact of duration and route of exposure on the transmission bottleneck in a hamster model
Barcoded SARS-CoV-2 viruses define the impact of duration and route of exposure on the transmission bottleneck in a hamster model Open
The transmission bottleneck, defined as the number of viruses shed from one host to infect another, is an important determinant of the rate of virus evolution and the level of immunity required to protect against virus transmission. Despit…
Genomic surveillance of SARS-CoV-2 in Nepal Open
During the COVID-19 pandemic, Nepal, like other countries, faced emerging SARS-CoV-2 variants. To evaluate the circulating variants, 278 samples collected between September 2021 and March 2022 were sequenced in the country. From these, 229…
Immune system benefits of pulmonary rehabilitation in chronic obstructive pulmonary disease Open
Chronic obstructive pulmonary disease (COPD) is a respiratory disease characterized by pulmonary and systemic inflammation. Inflammatory mediators show relationships with shortness of breath, exercise intolerance and health related quality…
Human immunodeficiency virus-1 genome from patient with fever, Nepal Open
A patient with fever presented to the referral infectious disease hospital in Kathmandu, Nepal. Metagenomic sequencing of the patient’s serum recovered a near-complete genome of human immunodeficiency virus-1 (HIV-1), distinct from previou…
Novel murine model of human astrovirus infection reveals a cardiovascular tropism Open
Astroviruses are a common cause of gastrointestinal disease in humans and have been recognized to cause fatal cases of encephalitis. A major barrier to the study of human-infecting astroviruses is the lack of an in vivo model, as previous …
eLife assessment: Bacteria are a major determinant of Orsay virus transmission and infection in Caenorhabditis elegans Open
Full text Figures and data Peer review Side by side Abstract eLife assessment Introduction Results Discussion Materials and methods Data availability References Article and author information Metrics Abstract The microbiota is a key determ…
Tracking the prevalence and emergence of SARS-CoV-2 variants of concern using a regional genomic surveillance program Open
SARS-CoV-2 molecular testing coupled with whole-genome sequencing is instrumental for real-time genomic surveillance. Genomic surveillance is critical for monitoring the spread of variants of concern (VOCs) as well as discovery of novel va…
Gemykibivirus detection in acute encephalitis patients from Nepal Open
Acute encephalitis syndrome (AES) causes significant morbidity and mortality worldwide. In Nepal, Japanese encephalitis virus (JEV) accounts for ~5-20% of AES cases, but ~75% of AES cases are of unknown etiology. We identified a gemykibivi…
View article: Barcoded SARS-CoV-2 viruses define the impact of time and route of transmission on the transmission bottleneck in a Syrian hamster model
Barcoded SARS-CoV-2 viruses define the impact of time and route of transmission on the transmission bottleneck in a Syrian hamster model Open
The transmission bottleneck, defined as the number of viruses that transmit from one host to infect another, is an important determinant of the rate of virus evolution and the level of immunity required to protect against virus transmissio…
Dengue Virus Surveillance in Nepal Yields the First On-Site Whole Genome Sequences of Isolates from the 2022 Outbreak Open
Background The 4 serotypes of dengue virus (DENV1-4) can each cause potentially deadly dengue disease, and are spreading globally from tropical and subtropical areas to more temperate ones. Nepal provides a microcosm of this global phenome…
Isolation of a recombinant simian adenovirus encoding the human adenovirus G52 hexon suggests a simian origin for human adenovirus G52 Open
Human adenoviruses (HAdVs) are causative agents of morbidity and mortality throughout the world. These double-stranded DNA viruses are phylogenetically classified into seven different species (A–G). HAdV-G52, originally isolated in 2008 fr…
Blockade of Interleukin-6 (IL-6) Signaling in Dedifferentiated Liposarcoma (DDLPS) Decreases Mouse Double Minute 2 (MDM2) Oncogenicity via Alternative Splicing Open
Effective therapies for retroperitoneal (RP) dedifferentiated liposarcoma (DDLPS) remain unavailable. Loco-regional recurrence occurs in >80% of cases; 5-year disease-specific survival is only 20%. DDLPS is especially prevalent in the retr…
Gemykibivirus detection in acute encephalitis patients from Nepal Open
Acute Encephalitis Syndrome (AES) causes significant morbidity and mortality worldwide. In Nepal, Japanese encephalitis virus (JEV) accounts for ∼ 5-20% of AES cases, but ∼75% of AES cases are of unknown etiology. We identified a gemykibiv…
Draft genomes of 12 <i>Bifidobacterium</i> isolates from human IBD fecal samples Open
Twelve Bifidobacterium strains were isolated from fecal samples of inflammatory bowel disease patients and matched “household control” individuals. These include the species Bifidobacterium adolescentis , Bifidobacterium animalis , Bifidob…
eLife assessment: Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegans Open
The microbiota is a key determinant of the physiology and antiviral immunity of animal hosts. The factors governing the transmissibility of viruses between susceptible hosts are incompletely understood. Bacteria serve as food for Caenorhab…
Picobirnaviruses encode proteins that are functional bacterial lysins Open
Picobirnaviruses (PBVs) are double-stranded RNA viruses frequently detected in human and animal enteric viromes. Associations of PBVs with enteric graft-versus-host disease and type I diabetes during pregnancy have been established. Since …
Host interactions of novel Crassvirales species belonging to multiple families infecting bacterial host, Bacteroides cellulosilyticus WH2 Open
Bacteroides, the prominent bacteria in the human gut, play a crucial role in degrading complex polysaccharides. Their abundance is influenced by phages belonging to the Crassvirales order. Despite identifying over 600 Crassvirales genomes …
Low- and high-grade glioma endothelial cells differentially regulate tumor growth Open
Background A key feature distinguishing high-grade glioma (HGG) from low-grade glioma (LGG) is the extensive neovascularization and endothelial hyperproliferation. Prior work has shown that tumor endothelial cells (TEC) from HGG are molecu…
View article: Paper 30: Lower Socioeconomic Status Adversely Affects Timing of Care and Rate of Re-Injury Following Anterior Cruciate Ligament Reconstruction
Paper 30: Lower Socioeconomic Status Adversely Affects Timing of Care and Rate of Re-Injury Following Anterior Cruciate Ligament Reconstruction Open
Objectives: The primary aim of this study was to define the impact of socioeconomic factors, such as national area of deprivation index (ADI) and medical insurance type, on the access to orthopaedic care after an ACL rupture. The secondary…
The Highly Conserved Stem-Loop II Motif Is Dispensable for SARS-CoV-2 Open
RNA viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), contain functional structures to support virus replication, translation, and evasion of the host antiviral immune response. The 3′ untranslated region of …
Tracking the prevalence and emergence of SARS CoV2 variants of concern using a regional genomic surveillance program Open
SARS-CoV-2 molecular testing coupled with whole genome sequencing is instrumental for real-time genomic surveillance. Genomic surveillance is critical for monitoring the spread of variants of concern (VOC) as well as novel variant discover…
The <i>alg-1</i> Gene Is Necessary for Orsay Virus Replication in Caenorhabditis elegans Open
All viruses are obligate intracellular parasites that recruit the cellular machinery of the host they infect to support their own proliferation. We used Caenorhabditis elegans and its only known infecting virus, Orsay virus, to identify ho…
The highly conserved stem-loop II motif is dispensable for SARS-CoV-2 Open
The stem-loop II motif (s2m) is a RNA structural element that is found in the 3’ untranslated region (UTR) of many RNA viruses including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Though the motif was discovered over twe…