Sven W. Meckelmann
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View article: Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis
Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis Open
Ageing dampens the regenerative potential of intestinal epithelium across species including humans, yet the underlying causes remain elusive. Here we characterized the temporal dynamics of regeneration following injury induced by 5-fluorou…
View article: Sterol analysis in cancer cells using atmospheric pressure ionization techniques
Sterol analysis in cancer cells using atmospheric pressure ionization techniques Open
The analysis of sterol biosynthesis poses analytical challenges such as vast concentration differences between precursor molecules and cholesterol as well as the substantial interferences between the sterols and the matrix, resulting in st…
View article: Ultrashort‐Pulsed Laser Diffusion Enhancement in Liquids for Cold Extraction of Coffee in an Analytically Validated Low‐Fluence Regime
Ultrashort‐Pulsed Laser Diffusion Enhancement in Liquids for Cold Extraction of Coffee in an Analytically Validated Low‐Fluence Regime Open
Coffee is one of the most popular beverages, characterized by a complex composition and individual flavor profiles, strongly affected by extraction methods. A continuous and scalable cold extraction method based on irradiation of coffee pa…
View article: Isoniazid-Modified Silica for Hydrophilic Interaction Chromatography and as an Adsorbent for Dye Removal in Wastewater Samples
Isoniazid-Modified Silica for Hydrophilic Interaction Chromatography and as an Adsorbent for Dye Removal in Wastewater Samples Open
The new isoniazid-derived material was synthesized with a surface-immobilizable functional group by a reaction between isoniazid and succinyl anhydride and grafted onto silica surfaces. The material was designed so that multiple functional…
View article: Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry
Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry Open
Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography–mass spectrometry (LC-MS) presents substantial technical challenges. As members of th…
View article: A <scp>CoA</scp> ‐Transferase and Acyl‐ <scp>CoA</scp> Dehydrogenase Convert 2‐(Carboxymethyl)cyclohexane‐1‐Carboxyl‐ <scp>CoA</scp> During Anaerobic Naphthalene Degradation
A <span>CoA</span> ‐Transferase and Acyl‐ <span>CoA</span> Dehydrogenase Convert 2‐(Carboxymethyl)cyclohexane‐1‐Carboxyl‐ <span>CoA</span> During Anaerobic Naphthalene Degradation Open
The CoA thioester of 2‐(carboxymethyl)cyclohexane‐1‐carboxylic acid has been identified as a metabolite in anaerobic naphthalene degradation by the sulfate‐reducing culture N47. This study identified and characterised two acyl‐CoA dehydrog…
View article: Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis
Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis Open
Mitochondrial DNA (mtDNA) mutations are frequent in cancer, yet their precise role in cancer progression remains debated. To functionally evaluate the impact of mtDNA variants on tumor growth and metastasis, we developed an enhanced cytopl…
View article: Investigation of the Cholesterol Biosynthesis by Heart-Cut Liquid Chromatography and Mass Spectrometric Detection
Investigation of the Cholesterol Biosynthesis by Heart-Cut Liquid Chromatography and Mass Spectrometric Detection Open
The biosynthesis and homeostasis of cholesterol are essential for cellular function. Cholesterol is a major lipid with multiple roles in membrane stability, signaling, or as a precursor for other molecules. Because of the structural simila…
View article: Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis
Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis Open
Aging hampers the regenerative potential of intestinal epithelium across species including humans, yet the underlying causes remain elusive. Here, using proteomic and metabolomic profiling of intestinal tissues together with functional ass…
View article: Automated green sample preparation for quantitative extraction of lipids in different sample matrices
Automated green sample preparation for quantitative extraction of lipids in different sample matrices Open
An essential step in all analytical workflows is the quantitative and robust extraction of the molecules of interest. While typical lipid extractions use hazardous solvents from non-renewable sources, in this work, an extraction protocol u…
View article: <i>De novo</i>synthesis of fatty acids in Archaea via an archaeal fatty acid synthase complex
<i>De novo</i>synthesis of fatty acids in Archaea via an archaeal fatty acid synthase complex Open
Archaea synthesize membranes using isoprenoid-based ether lipids, whereas Bacteria and Eukarya use fatty acid-based ester lipids. While the factors responsible for this “lipid divide” remain unclear, this has important implications for und…
View article: Fatty acids are crucial to fuel NK cells upon acute retrovirus infection
Fatty acids are crucial to fuel NK cells upon acute retrovirus infection Open
Natural killer (NK) cells are cytotoxic innate immune cells, able to recognize and eliminate virus-infected as well as cancer cells. Metabolic reprogramming is crucial for their activity as they have enhanced energy and nutritional demands…
View article: Surface Modification of Silica with β-Alanine Derivatives for Unique Applications in Liquid Chromatography
Surface Modification of Silica with β-Alanine Derivatives for Unique Applications in Liquid Chromatography Open
Column purchasing cost is an important issue for an analyst to analyze complex sample matrices. Here, we report the development of an amino acid (β-alanine)-derived stationary phase (Sil-Ala-C12) with strategic and effective interaction si…
View article: An overview of food lipids toward food lipidomics
An overview of food lipids toward food lipidomics Open
Increasing evidence regarding lipids’ beneficial effects on human health has changed the common perception of consumers and dietary officials about the role(s) of food lipids in a healthy diet. However, lipids are a wide group of molecules…
View article: Review on atmospheric pressure ionization sources for gas chromatography-mass spectrometry. Part II: Current applications
Review on atmospheric pressure ionization sources for gas chromatography-mass spectrometry. Part II: Current applications Open
The application of atmospheric pressure ionization (API) sources in gas chromatography-mass spectrometry (GC-MS) determinations is becoming more popular since they have shown great capabilities to sort out the main drawbacks of vacuum ioni…
View article: Review on atmospheric pressure ionization sources for gas chromatography-mass spectrometry. Part I: Current ion source developments and improvements in ionization strategies
Review on atmospheric pressure ionization sources for gas chromatography-mass spectrometry. Part I: Current ion source developments and improvements in ionization strategies Open
The use of atmospheric pressure ionization (API) sources has become very popular for gas chromatography-mass spectrometry (GC-MS) determinations. GC-API-MS shows important advantages over traditional vacuum ionization sources such as a low…
View article: Potential of atmospheric pressure ionization sources for the analysis of free fatty acids in clinical and biological samples by gas chromatography-mass spectrometry
Potential of atmospheric pressure ionization sources for the analysis of free fatty acids in clinical and biological samples by gas chromatography-mass spectrometry Open
Because of the central role of fatty acids in biological systems, their accurate quantification is still important. However, the impact of the complex matrix of biologically and clinically relevant samples such as plasma, serum, or cells m…
View article: Characterization of the Extracellular Volatile Metabolome of Pseudomonas Aeruginosa Applying an in vitro Biofilm Model under Cystic Fibrosis-Like Conditions
Characterization of the Extracellular Volatile Metabolome of Pseudomonas Aeruginosa Applying an in vitro Biofilm Model under Cystic Fibrosis-Like Conditions Open
Background: Cystic fibrosis (CF) is an autosomal recessive hereditary disease that leads to the production of thickened mucus in the lungs, favouring polymicrobial infections, such as chronic lung infections with the bacterial opportunisti…
View article: Oxylipin metabolism is controlled by mitochondrial β-oxidation during bacterial inflammation
Oxylipin metabolism is controlled by mitochondrial β-oxidation during bacterial inflammation Open
Oxylipins are potent biological mediators requiring strict control, but how they are removed en masse during infection and inflammation is unknown. Here we show that lipopolysaccharide (LPS) dynamically enhances oxylipin removal via mitoch…
View article: Oxylipin metabolism is controlled by mitochondrial β-oxidation during bacterial inflammation
Oxylipin metabolism is controlled by mitochondrial β-oxidation during bacterial inflammation Open
Oxylipins are potent biological mediators requiring strict control, but how they are removed en masse during infection and inflammation is unknown. Here we show that lipopolysaccharide (LPS) dynamically enhances oxylipin removal via mitoch…
View article: Metabolic Dysregulation of the Lysophospholipid/Autotaxin Axis in the Chromosome 9p21 Gene SNP rs10757274
Metabolic Dysregulation of the Lysophospholipid/Autotaxin Axis in the Chromosome 9p21 Gene SNP rs10757274 Open
Background: Common chromosome 9p21 single nucleotide polymorphisms (SNPs) increase coronary heart disease risk, independent of traditional lipid risk factors. However, lipids comprise large numbers of structurally related molecules not mea…